LOCUS       BBa_K660609              821 bp    DNA     linear       17-May-2024
DEFINITION  Bluf Promoter + S. RBS + RFP
ACCESSION   BBa_K660609
VERSION     BBa_K660609.1  
FEATURES             Location/Qualifiers
     promoter        28..33
                     /label=-35 box
     promoter        52..58
                     /label=-10 box
     misc_feature    34..51
                     /label=spacer
     misc_feature    53..59
                     /label=inverted repeat part 1
     misc_feature    67..73
                     /label=inverted repeat part 2
     misc_feature    64..65
                     /label=transcription start
     promoter        1..86
                     /label=BBa_K238013
     RBS             95..109
                     /label=RBS-1\Strong
     RBS             102..106
                     /label=RBS
     misc_feature    95..109
                     /label=BBa_B0030
     RBS             95..109
                     /label=BBa_B0030
     CDS             116..790
                     /label=mrfp1
     misc_feature    797..821
                     /label=Help:Barcodes
     CDS             116..821
                     /label=BBa_E1010
ORIGIN
        1 attgcaaaaa attaatttat cattctgtac acatatttcg tacaagtttg ctattgttac
       61 ttcacttaac attgattaac attttttact agagattaaa gaggagaaat actagatggc
      121 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
      181 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
      241 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
      301 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
      361 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
      421 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
      481 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
      541 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
      601 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
      661 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
      721 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
      781 caccggtgct taataacgct gatagtgcta gtgtagatcg c
//