LOCUS       BBa_K663000             1947 bp    DNA     linear       14-Nov-2024
DEFINITION  Homoserine lactone sensor
ACCESSION   BBa_K663000
VERSION     BBa_K663000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     RBS             1070..1084
                     /label=RBS-1\Strong
     RBS             1077..1081
                     /label=RBS
     misc_feature    1070..1084
                     /label=BBa_B0030
     RBS             1070..1084
                     /label=BBa_B0030
     CDS             1091..1810
                     /label=GFP protein
     CDS             1091..1810
                     /label=BBa_E0040
     stem_loop       1830..1873
                     /label=stem_loop
     misc_feature    1819..1898
                     /label=BBa_B0010
     terminator      1819..1898
                     /label=BBa_B0010
     stem_loop       1914..1933
                     /label=T7 TE
     misc_feature    1940..1940
                     /label=stop
     polyA_site      1934..1947
                     /label=polya
     misc_feature    1907..1947
                     /label=BBa_B0012
     terminator      1907..1947
                     /label=BBa_B0012
     misc_feature    1070..1947
                     /label=BBa_E0840
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ttaaagagga
     1081 gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     1141 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     1201 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     1261 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
     1321 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     1381 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     1441 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     1501 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     1561 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     1621 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     1681 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     1741 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     1801 caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1861 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1921 cttcgggtgg gcctttctgc gtttata
//