LOCUS BBa_K663002 4254 bp DNA linear 25-Nov-2024 DEFINITION Wintergreen and GFP-generators on an homoserine lactone responsive promoter ACCESSION BBa_K663002 VERSION BBa_K663002.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 RBS 1070..1084 /label=RBS-1\Strong RBS 1077..1081 /label=RBS misc_feature 1070..1084 /label=BBa_B0030 RBS 1070..1084 /label=BBa_B0030 CDS 1091..1810 /label=GFP protein CDS 1091..1810 /label=BBa_E0040 stem_loop 1830..1873 /label=stem_loop misc_feature 1819..1898 /label=BBa_B0010 terminator 1819..1898 /label=BBa_B0010 stem_loop 1914..1933 /label=T7 TE misc_feature 1940..1940 /label=stop polyA_site 1934..1947 /label=polya misc_feature 1907..1947 /label=BBa_B0012 terminator 1907..1947 /label=BBa_B0012 misc_feature 1070..1947 /label=BBa_E0840 misc_feature 1..1947 /label=BBa_K663000 misc_feature 1956..1974 /label=TetR 1 misc_feature 1956..2009 /label=BBa_R0040 promoter 1975..1980 /label=-35 misc_feature 1981..1999 /label=TetR 2 promoter 1998..2003 /label=-10 promoter 1956..2009 /label=BBa_R0040 misc_feature 2022..2025 /label=conserved RBS 2018..2029 /label=BBa_B0034 misc_feature 2036..2037 /label=prefix misc_feature 2209..2209 /label=T misc_feature 2527..2527 /label=A CDS 2036..2785 /label=luxR misc_feature 2036..2791 /label=BBa_C0062 misc_feature 2792..2816 /label=Help:Barcodes CDS 2036..2791 /label=BBa_C0062 stem_loop 2836..2879 /label=stem_loop misc_feature 2825..2904 /label=BBa_B0010 terminator 2825..2904 /label=BBa_B0010 stem_loop 2920..2939 /label=T7 TE misc_feature 2946..2946 /label=stop polyA_site 2940..2953 /label=polya misc_feature 2913..2953 /label=BBa_B0012 terminator 2913..2953 /label=BBa_B0012 terminator 2825..2953 /label=BBa_B0015 misc_feature 2962..2981 /label=LuxR/HSL promoter 2981..2986 /label=-35 promoter 3003..3008 /label=-10 misc_feature 3014..3014 /label=start misc_feature 2962..3016 /label=BBa_R0062 promoter 2962..3016 /label=BBa_R0062 misc_feature 1956..3016 /label=BBa_F2620 RBS 3025..3037 /label=RBS-3\Weak RBS 3031..3034 /label=RBS misc_feature 3025..3037 /label=BBa_B0032 RBS 3025..3037 /label=BBa_B0032 CDS 3044..4117 /label=BMST1 misc_feature 3044..3046 /label=start codon misc_feature 4115..4117 /label=stop codon CDS 3044..4117 /label=BBa_J45004 stem_loop 4137..4180 /label=stem_loop misc_feature 4126..4205 /label=BBa_B0010 terminator 4126..4205 /label=BBa_B0010 stem_loop 4221..4240 /label=T7 TE misc_feature 4247..4247 /label=stop polyA_site 4241..4254 /label=polya misc_feature 4214..4254 /label=BBa_B0012 terminator 4214..4254 /label=BBa_B0012 misc_feature 3025..4254 /label=BBa_J45119 misc_feature 1956..4254 /label=BBa_K663001 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ttaaagagga 1081 gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga 1141 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc 1201 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg 1261 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca 1321 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac 1381 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga 1441 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct 1501 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca 1561 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca 1621 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga 1681 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca 1741 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata 1801 caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 1861 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 1921 cttcgggtgg gcctttctgc gtttatatac tagagtccct atcagtgata gagattgaca 1981 tccctatcag tgatagagat actgagcact actagagaaa gaggagaaat actagatgaa 2041 aaacataaat gccgacgaca catacagaat aattaataaa attaaagctt gtagaagcaa 2101 taatgatatt aatcaatgct tatctgatat gactaaaatg gtacattgtg aatattattt 2161 actcgcgatc atttatcctc attctatggt taaatctgat atttcaatcc tagataatta 2221 ccctaaaaaa tggaggcaat attatgatga cgctaattta ataaaatatg atcctatagt 2281 agattattct aactccaatc attcaccaat taattggaat atatttgaaa acaatgctgt 2341 aaataaaaaa tctccaaatg taattaaaga agcgaaaaca tcaggtctta tcactgggtt 2401 tagtttccct attcatacgg ctaacaatgg cttcggaatg cttagttttg cacattcaga 2461 aaaagacaac tatatagata gtttattttt acatgcgtgt atgaacatac cattaattgt 2521 tccttctcta gttgataatt atcgaaaaat aaatatagca aataataaat caaacaacga 2581 tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc gaaggaaaaa gctcttggga 2641 tatttcaaaa atattaggtt gcagtgagcg tactgtcact ttccatttaa ccaatgcgca 2701 aatgaaactc aatacaacaa accgctgcca aagtatttct aaagcaattt taacaggagc 2761 aattgattgc ccatacttta aaaattaata acactgatag tgctagtgta gatcactact 2821 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 2881 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 2941 ttctgcgttt atatactaga gacctgtagg atcgtacagg tttacgcaag aaaatggttt 3001 gttatagtcg aataaatact agagtcacac aggaaagtac tagatggaag ttgttgaagt 3061 tcttcacatg aatggaggaa atggagacag tagctatgca aacaattctt tggttcagca 3121 aaaggtgatt ctcatgacaa agccaataac tgagcaagcc atgattgatc tctacagcag 3181 cctctttcca gaaaccttat gcattgcaga tttgggttgt tctttgggag ctaacacttt 3241 cttggtggtc tcacagcttg ttaaaatagt agaaaaagaa cgaaaaaagc atggttttaa 3301 gtctccagag ttttattttc acttcaatga tcttcctggc aatgatttta atacactttt 3361 tcagtcactg ggggcatttc aagaagattt gagaaagcat ataggggaaa gctttggtcc 3421 atgttttttc agtggagtgc ctggttcatt ttatactaga cttttccctt ccaaaagttt 3481 acattttgtt tactcctcct acagtctcat gtggctatct caggtgccta atgggattga 3541 aaataacaag ggaaacattt acatggcaag aacaagccct ctaagtgtta ttaaagcata 3601 ctacaagcaa tatgaaatag atttttcaaa ttttctcaag taccgttcag aggaattgat 3661 gaaaggtgga aagatggtgt taacactcct aggtagagaa agtgaggatc ctactagcaa 3721 agaatgctgt tacatttggg agcttctagc catggccctc aataagttgg ttgaagaggg 3781 attgataaaa gaagagaaag tagatgcatt caatattcct caatacacac catcaccagc 3841 agaagtaaag tacatagttg agaaggaagg atcattcacc attaatcgct tggaaacatc 3901 aagagttcat tggaatgctt ctaataatga gaagaatggt ggttacaatg tgtcaaggtg 3961 catgagagct gtggctgagc ctttgcttgt cagccacttt gacaaggaat tgatggattt 4021 agtgttccac aagtacgaag agattgtttc tgattgcatg tccaaagaga atactgagtt 4081 tataaatgtc atcatctcct tgaccaaaat aaattaatac tagagccagg catcaaataa 4141 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 4201 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //