##gff-version 3
##sequence-region BBa_K663002 1 4254
BBa_K663002	.	engineered_region	1	1947	.	+	0	ID=BBa_K663000;Name=BBa_K663000
BBa_K663002	.	engineered_region	1070	1947	.	+	0	ID=BBa_E0840;Name=BBa_E0840;Parent=BBa_K663000
BBa_K663002	.	terminator	1907	1947	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K663002	.	polyA_site	1934	1947	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K663002	.	engineered_region	1907	1947	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K663002	.	stem_loop	1914	1933	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K663002	.	stop_codon	1940	1940	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K663002	.	ribosome_entry_site	1070	1084	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K663002	.	ribosome_entry_site	1070	1084	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K663002	.	ribosome_entry_site	1077	1081	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K663002	.	engineered_region	1070	1084	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K663002	.	CDS	1091	1810	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K663002	.	CDS	1091	1810	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K663002	.	terminator	1819	1898	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K663002	.	stem_loop	1830	1873	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K663002	.	engineered_region	1819	1898	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K663002	.	engineered_region	1	1061	.	+	0	ID=BBa_F2620;Name=BBa_F2620;Parent=BBa_K663000
BBa_K663002	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_K663002	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K663002	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K663002	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K663002	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K663002	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K663002	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K663002	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_K663002	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K663002	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K663002	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K663002	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K663002	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K663002	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_K663002	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K663002	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K663002	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K663002	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K663002	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K663002	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_K663002	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K663002	.	terminator	870	998	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_K663002	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K663002	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K663002	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K663002	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K663002	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K663002	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K663002	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K663002	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K663002	.	engineered_region	1956	4254	.	+	0	ID=BBa_K663001;Name=BBa_K663001
BBa_K663002	.	engineered_region	1956	3016	.	+	0	ID=BBa_F2620;Name=BBa_F2620;Parent=BBa_K663001
BBa_K663002	.	CDS	2036	2791	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_K663002	.	CDS	2036	2785	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K663002	.	engineered_tag	2792	2816	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K663002	.	engineered_region	2036	2791	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K663002	.	point_mutation	2209	2209	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K663002	.	engineered_region	2036	2037	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K663002	.	point_mutation	2527	2527	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K663002	.	promoter	2962	3016	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_K663002	.	engineered_region	2962	3016	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K663002	.	promoter	2981	2986	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K663002	.	promoter	3003	3008	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K663002	.	start_codon	3014	3014	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K663002	.	non_covalent_binding_site	2962	2981	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K663002	.	promoter	1956	2009	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_K663002	.	engineered_region	1956	2009	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K663002	.	non_covalent_binding_site	1956	1974	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K663002	.	non_covalent_binding_site	1981	1999	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K663002	.	promoter	1975	1980	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K663002	.	promoter	1998	2003	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K663002	.	ribosome_entry_site	2018	2029	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_K663002	.	conserved_region	2022	2025	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K663002	.	terminator	2825	2953	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_K663002	.	terminator	2913	2953	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K663002	.	polyA_site	2940	2953	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K663002	.	engineered_region	2913	2953	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K663002	.	stem_loop	2920	2939	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K663002	.	stop_codon	2946	2946	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K663002	.	terminator	2825	2904	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K663002	.	stem_loop	2836	2879	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K663002	.	engineered_region	2825	2904	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K663002	.	engineered_region	3025	4254	.	+	0	ID=BBa_J45119;Name=BBa_J45119;Parent=BBa_K663001
BBa_K663002	.	terminator	4214	4254	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45119
BBa_K663002	.	polyA_site	4241	4254	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K663002	.	engineered_region	4214	4254	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K663002	.	stem_loop	4221	4240	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K663002	.	stop_codon	4247	4247	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K663002	.	terminator	4126	4205	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45119
BBa_K663002	.	stem_loop	4137	4180	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K663002	.	engineered_region	4126	4205	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K663002	.	CDS	3044	4117	.	+	0	ID=BBa_J45004;Name=BBa_J45004;Parent=BBa_J45119
BBa_K663002	.	start_codon	3044	3046	.	+	0	ID=annotation1880519;Name=start codon;Parent=BBa_J45004
BBa_K663002	.	CDS	3044	4117	.	+	0	ID=annotation1880517;Name=BMST1;Parent=BBa_J45004
BBa_K663002	.	stop_codon	4115	4117	.	+	0	ID=annotation1880520;Name=stop codon;Parent=BBa_J45004
BBa_K663002	.	ribosome_entry_site	3025	3037	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J45119
BBa_K663002	.	engineered_region	3025	3037	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K663002	.	ribosome_entry_site	3025	3037	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K663002	.	ribosome_entry_site	3031	3034	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_K663002
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatgcgtaaaggagaag
aacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggcca
acacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttca
agagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctga
agtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattctt
ggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagtta
acttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcga
tggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagac
cacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagat
tgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccg
acgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgac
taaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatccta
gataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattcta
actccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaaga
agcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagtttt
gcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctc
tagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgttt
agcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccat
ttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattg
attgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgt
tatagtcgaataaatactagagtcacacaggaaagtactagatggaagttgttgaagttcttcacatgaatggaggaaa
tggagacagtagctatgcaaacaattctttggttcagcaaaaggtgattctcatgacaaagccaataactgagcaagcc
atgattgatctctacagcagcctctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaacactt
tcttggtggtctcacagcttgttaaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttttattt
tcacttcaatgatcttcctggcaatgattttaatacactttttcagtcactgggggcatttcaagaagatttgagaaag
catataggggaaagctttggtccatgttttttcagtggagtgcctggttcattttatactagacttttcccttccaaaa
gtttacattttgtttactcctcctacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaagggaaa
catttacatggcaagaacaagccctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaaatttt
ctcaagtaccgttcagaggaattgatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgaggatccta
ctagcaaagaatgctgttacatttgggagcttctagccatggccctcaataagttggttgaagagggattgataaaaga
agagaaagtagatgcattcaatattcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaaggaagga
tcattcaccattaatcgcttggaaacatcaagagttcattggaatgcttctaataatgagaagaatggtggttacaatg
tgtcaaggtgcatgagagctgtggctgagcctttgcttgtcagccactttgacaaggaattgatggatttagtgttcca
caagtacgaagagattgtttctgattgcatgtccaaagagaatactgagtttataaatgtcatcatctccttgaccaaa
ataaattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata