LOCUS BBa_K669007 3744 bp DNA linear 22-Nov-2024 DEFINITION Allows the measurement of the ChiP promoter activity ACCESSION BBa_K669007 VERSION BBa_K669007.1 FEATURES Location/Qualifiers promoter 1..60 /label=promoter misc_feature 1..1113 /label=BBa_K669007 CDS 61..1113 /label=cds misc_feature 1122..1990 /label=BBa_J06702 misc_feature 1122..1133 /label=BBa_B0034 misc_feature 1126..1129 /label=conserved misc_feature 1140..1853 /label=BBa_J06504 CDS 1140..1850 /label=mCherry misc_feature 1491..1491 /label=C->T (removing PstI site) misc_feature 1862..1941 /label=BBa_B0010 stem_loop 1873..1916 /label=stem_loop misc_feature 1950..1990 /label=BBa_B0012 stem_loop 1957..1976 /label=T7 TE polyA_site 1977..1990 /label=polya misc_feature 1983..1983 /label=stop misc_feature 1999..2867 /label=BBa_J06702 misc_feature 1999..2010 /label=BBa_B0034 misc_feature 2003..2006 /label=conserved CDS 2017..2727 /label=mCherry misc_feature 2017..2730 /label=BBa_J06504 misc_feature 2368..2368 /label=C->T (removing PstI site) misc_feature 2739..2818 /label=BBa_B0010 stem_loop 2750..2793 /label=stem_loop misc_feature 2827..2867 /label=BBa_B0012 stem_loop 2834..2853 /label=T7 TE polyA_site 2854..2867 /label=polya misc_feature 2860..2860 /label=stop misc_feature 2876..2887 /label=BBa_B0034 misc_feature 2880..2883 /label=conserved CDS 2894..3604 /label=mCherry misc_feature 2894..3607 /label=BBa_J06504 misc_feature 3245..3245 /label=C->T (removing PstI site) misc_feature 3616..3695 /label=BBa_B0010 stem_loop 3627..3670 /label=stem_loop misc_feature 3704..3744 /label=BBa_B0012 stem_loop 3711..3730 /label=T7 TE polyA_site 3731..3744 /label=polya misc_feature 3737..3737 /label=stop misc_feature 2876..3744 /label=BBa_J06702 ORIGIN 1 gccaacttaa agccaataaa taatgaacac agctatatcc atttttagga gctttattcc 61 atggaaaaca aattttttaa aacaacgtta ttaggcgcag ctgttgcttt agcatctgct 121 ggtgtaacag caaaagaagt tggtattaac tctgacttta atgttgaagt gtacggtgtg 181 gcagcaatgt ctactgttaa ctacaataca actggtaatg gcgatggtac aaacggttat 241 gtattagaaa atgaatctcg aattggtttt cgtgcacata aagaaatgtt tgaaaatgta 301 ttagttacaa tgcaaattga gtctggttat gtagatagct ggcaaggtgg agagttaggt 361 gctcgtgata cttacctagg tctttctggt gattggggta atttgcgtgc aggtcgtgta 421 cttacacctt tatatgaaat tattgactgg cctttctcta accctggttt aggttctgta 481 tttgattggg gtggtgtaaa tgctcactat gatcgtcaat caaaccaagt tcgttacgac 541 tctcctaatt ttggtggttt aacatttgct ctttctgctg gtcgtgatgg aaacccaagt 601 ggttctgcaa ctcgtgattc tcgttttttc agtggtaatg tgaaatatac aatcagcaca 661 gtaacattaa tgggtgctgt tgaaggtggt tctgatttcc gcggtgttga tgataatgat 721 aatttaactt atttagttgg tgctgaagta ggtttacctg gtggctttag tttagctggc 781 gcatataaac aagaaagtgt agataaattc tctggcaaaa atgctgcggg tgtagcggtt 841 tctggtgaaa atgtagatca agggtctttt tctgtaattg gtcagtattg gcttgattca 901 ttaggtttta aagttggtta tgctgctaac ttagatgctg atggaataaa aacggttgga 961 ctaaaagaag aatctttgga ttcgaataca atttcagggc aagtaatgta tgtgcataat 1021 ggttttgttc catacctacg tgtagctggt cgtactgtag gagataaaga tactgatatc 1081 gtaactcgtg taggtttaga gtacggtttc taatactaga gaaagaggag aaatactaga 1141 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg 1201 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc 1261 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct 1321 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc 1381 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg 1441 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ttgcaggacg 1501 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa 1561 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg 1621 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg 1681 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca 1741 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac 1801 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa taatactaga 1861 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 1921 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 1981 tgcgtttata tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggaggat 2041 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 2101 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 2161 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 2221 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 2281 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 2341 gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta caaggtgaag 2401 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 2461 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 2521 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 2581 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 2641 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 2701 ggcggcatgg acgagctgta caagtaataa tactagagcc aggcatcaaa taaaacgaaa 2761 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 2821 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagaaaga 2881 ggagaaatac tagatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt 2941 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg 3001 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 3061 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc 3121 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt 3181 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 3241 ctccttgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc 3301 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta 3361 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg 3421 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg 3481 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt 3541 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa 3601 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 3661 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 3721 cgggtgggcc tttctgcgtt tata //