BBa_K670001 1 BBa_K670001 LuxC protein generator 2011-06-07T11:00:00Z 2015-05-08T01:13:03Z This is a composite part including the LuxC protein coding region encoded in part BBa_K670000. LuxC protein is used to produce light from bacterial cells. It is the first enzyme in the luxCDABE operon. false false _853_ 0 2568 41 Not in stock false Used a strong RBS to produce high levels of expression of the LuxC protein. false Meagan Lizarazo component2120803 1 BBa_B0015 component2120796 1 BBa_K670000 component2120793 1 BBa_B0034 annotation2120793 1 BBa_B0034 range2120793 1 1 12 annotation2120803 1 BBa_B0015 range2120803 1 1467 1595 annotation2120796 1 BBa_K670000 range2120796 1 19 1458 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K670000 1 BBa_K670000 LuxC (acyl-CoA reductase) 2011-06-07T11:00:00Z 2015-05-08T01:13:03Z http://www.ncbi.nlm.nih.gov/gene/3280765 Vibrio fischeri luxC protein coding region. This part is useful for producing light in bacteria from endogenous substrates. It is the first enzyme in the luxCDABE operon. false true _853_ 0 2568 41 Not in stock false Mutated the naturally ocurring TAG stop codon to TAA false Meagan Lizarazo annotation2120790 1 LuxC coding region range2120790 1 1 1440 annotation2120791 1 ATG start codon range2120791 1 1 3 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K670000_sequence 1 atgattaaatgtattccgatgataattaaaggcgtagttcaagattttgataataacgcttgtaaagaaataaatttagatagtggtaataaaattaaattatctcttcttactgaagattcagttttaagatcattaaattctaaagaaaaagttgatcttaacttaaatcagattgtaaatttcctatataccgtaggtcaacgatggaaaaatgaagaatataatcgaagacgaacttatattcgtgagttaaaaaaatatcttggatattctgatgaaatggctagattagaagccaattggatagctatgttattgtgttctaaaagtgctttgtatgacattgttaattacgatttgggatctatacacgtgttggatgaatggcttccgcgtggtgattgctatgttaaagcacaagcaaaaggtgtttctattcacttgttggctggtaatgttccactatcaggggtgacatctattttgcgtgctattttgacaaaaaatgaatgcataattaaaacctcttcttcagatccgtttactgcaacggctttggcttcgagttttattgatgttaatgcagagcatccaataactaaatcaatgtctgttatgtattggccgcataatgaagatatgactctacctcaaagaattatgaatcatgcagacattgttattgcttggggaggagaagaggcgattaaatgggcggcaaaacactcaccaccgcatgccgatgttcttaaatttggaccaaagaaaagtttaagcattattgaggagcctgaagacatggaggaagctgcaatgggggttgcccatgatatctgtttctatgatcagcaagcctgcttctcaactcaagacgtttattatatcggagaacatttgcctttatttttaagtgaacttgaaaaacagctagatcgatacgcaaagattttaccaaaaggtttaaaaaattttgatgaaaaagcggcgttttctcttaccgaaagagagggaatatttgctggatatgacgtaaaaaagggggataaccaagcttggttaatgattatatctcctacaaatagctctggaaatcagccattatctcgaagcgtgtatatacatcaagtctctgatattaatgaggtgcttccttttgtaaataaaaatagtacacaaaccgtctctatttacccttgggaggcttcactgaaatatagagacaaattagcgatgagtggtgctgagagaatcgttgaatcaggaatgaataatattttcagagttggaggagcacatgattcattatctcctctccagtatttagttaggtttacctcccatgaaagaccatttcattatacaaccaaggatgttgcggttgaaattgaacaaacgcgttacttagaagaagacaaatttttggtttttgtcccataa BBa_K670001_sequence 1 aaagaggagaaatactagatgattaaatgtattccgatgataattaaaggcgtagttcaagattttgataataacgcttgtaaagaaataaatttagatagtggtaataaaattaaattatctcttcttactgaagattcagttttaagatcattaaattctaaagaaaaagttgatcttaacttaaatcagattgtaaatttcctatataccgtaggtcaacgatggaaaaatgaagaatataatcgaagacgaacttatattcgtgagttaaaaaaatatcttggatattctgatgaaatggctagattagaagccaattggatagctatgttattgtgttctaaaagtgctttgtatgacattgttaattacgatttgggatctatacacgtgttggatgaatggcttccgcgtggtgattgctatgttaaagcacaagcaaaaggtgtttctattcacttgttggctggtaatgttccactatcaggggtgacatctattttgcgtgctattttgacaaaaaatgaatgcataattaaaacctcttcttcagatccgtttactgcaacggctttggcttcgagttttattgatgttaatgcagagcatccaataactaaatcaatgtctgttatgtattggccgcataatgaagatatgactctacctcaaagaattatgaatcatgcagacattgttattgcttggggaggagaagaggcgattaaatgggcggcaaaacactcaccaccgcatgccgatgttcttaaatttggaccaaagaaaagtttaagcattattgaggagcctgaagacatggaggaagctgcaatgggggttgcccatgatatctgtttctatgatcagcaagcctgcttctcaactcaagacgtttattatatcggagaacatttgcctttatttttaagtgaacttgaaaaacagctagatcgatacgcaaagattttaccaaaaggtttaaaaaattttgatgaaaaagcggcgttttctcttaccgaaagagagggaatatttgctggatatgacgtaaaaaagggggataaccaagcttggttaatgattatatctcctacaaatagctctggaaatcagccattatctcgaagcgtgtatatacatcaagtctctgatattaatgaggtgcttccttttgtaaataaaaatagtacacaaaccgtctctatttacccttgggaggcttcactgaaatatagagacaaattagcgatgagtggtgctgagagaatcgttgaatcaggaatgaataatattttcagagttggaggagcacatgattcattatctcctctccagtatttagttaggtttacctcccatgaaagaccatttcattatacaaccaaggatgttgcggttgaaattgaacaaacgcgttacttagaagaagacaaatttttggtttttgtcccataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z