LOCUS       BBa_K672000             2511 bp    DNA     linear       05-Nov-2024
DEFINITION  Nitrate sensing at low temperatures.
ACCESSION   BBa_K672000
VERSION     BBa_K672000.1  
FEATURES             Location/Qualifiers
     promoter        1..393
                     /label=HybB Cold Shock Promoter
     misc_feature    414..476
                     /label=OmpA
     RBS             402..407
                     /label=Ribosome Binding Site
     CDS             483..1517
                     /label=AoxA (Alternative Oxidase)
     misc_feature    394..401
                     /label=NotI Site
     misc_feature    477..482
                     /label=XmaI Site
     misc_feature    1..1517
                     /label=BBa_K410000
     misc_feature    1575..1590
                     /label=NarL-binding region
     misc_feature    1583..1605
                     /label=NsrR-binding region
     misc_feature    1591..1596
                     /label=promoter -35 region
     misc_feature    1614..1619
                     /label=promoter -10 region
     promoter        1526..1625
                     /label=BBa_K216005
     RBS             1634..1648
                     /label=RBS-1\Strong
     RBS             1641..1645
                     /label=RBS
     misc_feature    1634..1648
                     /label=BBa_B0030
     RBS             1634..1648
                     /label=BBa_B0030
     CDS             1655..2374
                     /label=GFP protein
     CDS             1655..2374
                     /label=BBa_E0040
     stem_loop       2394..2437
                     /label=stem_loop
     misc_feature    2383..2462
                     /label=BBa_B0010
     terminator      2383..2462
                     /label=BBa_B0010
     stem_loop       2478..2497
                     /label=T7 TE
     misc_feature    2504..2504
                     /label=stop
     polyA_site      2498..2511
                     /label=polya
     misc_feature    2471..2511
                     /label=BBa_B0012
     terminator      2471..2511
                     /label=BBa_B0012
     misc_feature    1526..2511
                     /label=BBa_K381001
ORIGIN
        1 cgccgctatg gactggataa agagatggta atggatttct ttcgtgagaa taattcctgt
       61 tctacgttgc gcttttttat ggccggttat cgcctcgaaa attgatcaaa catacgtatt
      121 atcttgcttt aattaattac actaatgctt cttcccttcg ttttagcgcc ccgccgcagt
      181 atcatgatat cgataaccat aataaatgtg tggtaaatgg cgcatcgatc gcattattga
      241 ttttgcgatt gaggcaaaat atatgccagg tcttcgcaac ggaataacta taaatgactg
      301 gagataacac cctcatccat tctcacggca ttaaccgtcg tgatttcatg aagctttgtg
      361 cagcattagc cgccaccatg gggttaagta gcagcggccg caggagatat catatgaaaa
      421 agacagctat cgcgattgca gtggcactgg ctggtttcgc taccgttgcg caagctcccg
      481 ggatgatgaa cagcaaactg gcagctctgc tgctgaaaca actgggttct gctaccgttc
      541 gtaccgtgac aatgggtcct ctgaatggga ttaccactga gtcctcgtgc tttctgcatg
      601 catctggtcc ggtcgttcca ggtcgccgta cttggattcg cttttcttgt ctgggagtcc
      661 gtaatggttc taccagtgcc ctgaataaca aagagaaaga ggagaaaggc gtccgtacaa
      721 gttctactgt tggtggcgcc aatcgccctg aggataaaat gatcgtgtcc tattggggta
      781 tgcctccggc aaatctgact aaaaaagacg gctccgagtg gaaatggaac tcattccgtc
      841 catgggaaac ctataaagcc gatctgtcga tcgatctgaa aaaacaccat agcccggtga
      901 cgtttatgga taaactggcc tattggaccg ttaaagccct gcgctatccg actgatattc
      961 tgttccaaaa ccgttatggc tgtcgtgcca tgatgctgga gactgtggcg gcagtaccag
     1021 ggatggttgg ggggatgctg ctgcacctga aatctctgcg tcgcttcgaa cattctggtg
     1081 ggtggatcaa aacactgctg gaagaagccg aaaacgagcg tatgcacctg atgaccttca
     1141 tggaagtgtc tcagccaaaa tggtatgaac gtgccctggt tgtagcagtt cagggtgtgt
     1201 tcttcaacac gtattttctg ggttatctga tcagccctcg cttcgctcat cgtgtggttg
     1261 gatatctgga ggaggaagca attcactcct ataccgagtt tctgaaagaa ctggacaaag
     1321 gcaacatcca gaacgtccca gcaccagcaa ttgccgtgga ttattggcaa ctgccaccgg
     1381 attctacgct gcgtgatgtg gtgatggtcg tccgtgctga tgaagcacat catcgtgacg
     1441 tgaaccactt tgccagcgac attcacgacc aaggctatga gctgaaagag tctcctgctc
     1501 ctctgggata tcattaatac tagagttccc atctataatc ctccctgatt cttcgctgat
     1561 atggtgctaa aaagtaacca ataaatggta tttaaaatgc aaattatcag gcgtaccctg
     1621 aaacgtacta gagattaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac
     1681 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
     1741 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
     1801 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca
     1861 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
     1921 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
     1981 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
     2041 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
     2101 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
     2161 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
     2221 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
     2281 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
     2341 tacacatggc atggatgaac tatacaaata ataatactag agccaggcat caaataaaac
     2401 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2461 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//