##gff-version 3
##sequence-region BBa_K672000 1 2511
BBa_K672000	.	engineered_region	1	1517	.	+	0	ID=BBa_K410000;Name=BBa_K410000
BBa_K672000	.	promoter	1	393	.	+	0	ID=annotation2111504;Name=HybB Cold Shock Promoter;Parent=BBa_K410000
BBa_K672000	.	sequence_feature	477	482	.	+	0	ID=annotation2111736;Name=XmaI Site;Parent=BBa_K410000
BBa_K672000	.	ribosome_entry_site	402	407	.	+	0	ID=annotation2111580;Name=Ribosome Binding Site;Parent=BBa_K410000
BBa_K672000	.	sequence_feature	394	401	.	+	0	ID=annotation2111710;Name=NotI Site;Parent=BBa_K410000
BBa_K672000	.	tag	414	476	.	+	0	ID=annotation2111563;Name=OmpA;Parent=BBa_K410000
BBa_K672000	.	CDS	483	1517	.	+	0	ID=annotation2111604;Name=AoxA (Alternative Oxidase);Parent=BBa_K410000
BBa_K672000	.	engineered_region	1526	2511	.	+	0	ID=BBa_K381001;Name=BBa_K381001
BBa_K672000	.	CDS	1655	2374	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K381001
BBa_K672000	.	CDS	1655	2374	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K672000	.	ribosome_entry_site	1634	1648	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K381001
BBa_K672000	.	ribosome_entry_site	1634	1648	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K672000	.	engineered_region	1634	1648	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K672000	.	ribosome_entry_site	1641	1645	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K672000	.	terminator	2471	2511	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K381001
BBa_K672000	.	stem_loop	2478	2497	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K672000	.	stop_codon	2504	2504	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K672000	.	polyA_site	2498	2511	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K672000	.	engineered_region	2471	2511	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K672000	.	terminator	2383	2462	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K381001
BBa_K672000	.	stem_loop	2394	2437	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K672000	.	engineered_region	2383	2462	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K672000	.	promoter	1526	1625	.	+	0	ID=BBa_K216005;Name=BBa_K216005;Parent=BBa_K381001
BBa_K672000	.	non_covalent_binding_site	1583	1605	.	+	0	ID=annotation2027073;Name=NsrR-binding region;Parent=BBa_K216005
BBa_K672000	.	non_covalent_binding_site	1614	1619	.	+	0	ID=annotation2027075;Name=promoter -10 region;Parent=BBa_K216005
BBa_K672000	.	non_covalent_binding_site	1591	1596	.	+	0	ID=annotation2027074;Name=promoter -35 region;Parent=BBa_K216005
BBa_K672000	.	non_covalent_binding_site	1575	1590	.	+	0	ID=annotation2027072;Name=NarL-binding region;Parent=BBa_K216005
>BBa_K672000
cgccgctatggactggataaagagatggtaatggatttctttcgtgagaataattcctgttctacgttgcgctttttta
tggccggttatcgcctcgaaaattgatcaaacatacgtattatcttgctttaattaattacactaatgcttcttccctt
cgttttagcgccccgccgcagtatcatgatatcgataaccataataaatgtgtggtaaatggcgcatcgatcgcattat
tgattttgcgattgaggcaaaatatatgccaggtcttcgcaacggaataactataaatgactggagataacaccctcat
ccattctcacggcattaaccgtcgtgatttcatgaagctttgtgcagcattagccgccaccatggggttaagtagcagc
ggccgcaggagatatcatatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgttgcgcaag
ctcccgggatgatgaacagcaaactggcagctctgctgctgaaacaactgggttctgctaccgttcgtaccgtgacaat
gggtcctctgaatgggattaccactgagtcctcgtgctttctgcatgcatctggtccggtcgttccaggtcgccgtact
tggattcgcttttcttgtctgggagtccgtaatggttctaccagtgccctgaataacaaagagaaagaggagaaaggcg
tccgtacaagttctactgttggtggcgccaatcgccctgaggataaaatgatcgtgtcctattggggtatgcctccggc
aaatctgactaaaaaagacggctccgagtggaaatggaactcattccgtccatgggaaacctataaagccgatctgtcg
atcgatctgaaaaaacaccatagcccggtgacgtttatggataaactggcctattggaccgttaaagccctgcgctatc
cgactgatattctgttccaaaaccgttatggctgtcgtgccatgatgctggagactgtggcggcagtaccagggatggt
tggggggatgctgctgcacctgaaatctctgcgtcgcttcgaacattctggtgggtggatcaaaacactgctggaagaa
gccgaaaacgagcgtatgcacctgatgaccttcatggaagtgtctcagccaaaatggtatgaacgtgccctggttgtag
cagttcagggtgtgttcttcaacacgtattttctgggttatctgatcagccctcgcttcgctcatcgtgtggttggata
tctggaggaggaagcaattcactcctataccgagtttctgaaagaactggacaaaggcaacatccagaacgtcccagca
ccagcaattgccgtggattattggcaactgccaccggattctacgctgcgtgatgtggtgatggtcgtccgtgctgatg
aagcacatcatcgtgacgtgaaccactttgccagcgacattcacgaccaaggctatgagctgaaagagtctcctgctcc
tctgggatatcattaatactagagttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaacca
ataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacgtactagagattaaagaggagaaatactagatgcg
taaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttct
gtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctg
ttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagca
tgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaag
acacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatg
gaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatgg
aatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatact
ccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacg
aaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaata
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata