LOCUS       BBa_K672002             2439 bp    DNA     linear       08-May-2024
DEFINITION  Nitrate sensing at low temperatures
ACCESSION   BBa_K672002
VERSION     BBa_K672002.1  
FEATURES             Location/Qualifiers
     promoter        1..321
                     /label=CsPA+ColdBox+UTR+SD+DB
     misc_feature    322..329
                     /label=Notl Site
     RBS             330..335
                     /label=RBS
     misc_feature    342..404
                     /label=OmpA
     misc_feature    405..410
                     /label=Xmal Site
     CDS             411..1445
                     /label=Alternative Oxidase
     misc_feature    1..1445
                     /label=BBa_K672001
     misc_feature    1503..1518
                     /label=NarL-binding region
     misc_feature    1511..1533
                     /label=NsrR-binding region
     misc_feature    1519..1524
                     /label=promoter -35 region
     misc_feature    1542..1547
                     /label=promoter -10 region
     promoter        1454..1553
                     /label=BBa_K216005
     RBS             1562..1576
                     /label=RBS-1\Strong
     RBS             1569..1573
                     /label=RBS
     misc_feature    1562..1576
                     /label=BBa_B0030
     RBS             1562..1576
                     /label=BBa_B0030
     CDS             1583..2302
                     /label=GFP protein
     CDS             1583..2302
                     /label=BBa_E0040
     stem_loop       2322..2365
                     /label=stem_loop
     misc_feature    2311..2390
                     /label=BBa_B0010
     terminator      2311..2390
                     /label=BBa_B0010
     stem_loop       2406..2425
                     /label=T7 TE
     misc_feature    2432..2432
                     /label=stop
     polyA_site      2426..2439
                     /label=polya
     misc_feature    2399..2439
                     /label=BBa_B0012
     terminator      2399..2439
                     /label=BBa_B0012
     misc_feature    1454..2439
                     /label=BBa_K381001
ORIGIN
        1 cggcattaag taagcagttg atggaataga cttttatcca ctttattgct gtttacggtc
       61 ctgatgacag gaccgttttc caaccgatta atcataaata tgaaaaataa ttgttgcatc
      121 acccgccaat gcgtggctta atgcacatca acggtttgac gtacagacca ttaaagcagt
      181 gtagtaaggc aagtcccttc aagagttatc gttgataccc ctcgtagtgc acattccttt
      241 aacgcttcaa aatctgtaaa gcacgccata tcgccgaaag gcacacttaa ttattaaagg
      301 taatacacta tgtccggtaa agcggccgca ggagatatca tatgaaaaag acagctatcg
      361 cgattgcagt ggcactggct ggtttcgcta ccgttgcgca agctcccggg atgatgaaca
      421 gcaaactggc agctctgctg ctgaaacaac tgggttctgc taccgttcgt accgtgacaa
      481 tgggtcctct gaatgggatt accactgagt cctcgtgctt tctgcatgca tctggtccgg
      541 tcgttccagg tcgccgtact tggattcgct tttcttgtct gggagtccgt aatggttcta
      601 ccagtgccct gaataacaaa gagaaagagg agaaaggcgt ccgtacaagt tctactgttg
      661 gtggcgccaa tcgccctgag gataaaatga tcgtgtccta ttggggtatg cctccggcaa
      721 atctgactaa aaaagacggc tccgagtgga aatggaactc attccgtcca tgggaaacct
      781 ataaagccga tctgtcgatc gatctgaaaa aacaccatag cccggtgacg tttatggata
      841 aactggccta ttggaccgtt aaagccctgc gctatccgac tgatattctg ttccaaaacc
      901 gttatggctg tcgtgccatg atgctggaga ctgtggcggc agtaccaggg atggttgggg
      961 ggatgctgct gcacctgaaa tctctgcgtc gcttcgaaca ttctggtggg tggatcaaaa
     1021 cactgctgga agaagccgaa aacgagcgta tgcacctgat gaccttcatg gaagtgtctc
     1081 agccaaaatg gtatgaacgt gccctggttg tagcagttca gggtgtgttc ttcaacacgt
     1141 attttctggg ttatctgatc agccctcgct tcgctcatcg tgtggttgga tatctggagg
     1201 aggaagcaat tcactcctat accgagtttc tgaaagaact ggacaaaggc aacatccaga
     1261 acgtcccagc accagcaatt gccgtggatt attggcaact gccaccggat tctacgctgc
     1321 gtgatgtggt gatggtcgtc cgtgctgatg aagcacatca tcgtgacgtg aaccactttg
     1381 ccagcgacat tcacgaccaa ggctatgagc tgaaagagtc tcctgctcct ctgggatatc
     1441 attaatacta gagttcccat ctataatcct ccctgattct tcgctgatat ggtgctaaaa
     1501 agtaaccaat aaatggtatt taaaatgcaa attatcaggc gtaccctgaa acgtactaga
     1561 gattaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
     1621 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
     1681 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
     1741 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
     1801 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
     1861 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
     1921 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
     1981 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
     2041 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
     2101 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
     2161 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
     2221 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     2281 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt
     2341 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2401 acactggctc accttcgggt gggcctttct gcgtttata
//