http://partsregistry.org/wiki/index.php?title=Part:BBa_K672001 (New Promoter For Periplasmic Heat Generation) http://partsregistry.org/Part:BBa_K381001 (Nitrate reporter: PyeaR - GFP composite)
This part consists of the combination of our part BBa_K672001 + the part BBa_K381001 which was designed by iGEM10_BCCS-Bristol. It's purpose is to combine the nitrate sensing capabilities of Bristol's part with our heat production at temperatures of aproximately 15??C, which leads to have a bacteria that survives and senses nitrate at low temperatures.
New Promoter For Periplasmic Heat Generation
http://partsregistry.org/Part:BBa_K410000 (hybB-ompA-AOX1A) http://partsregistry.org/Part:BBa_K328000 (CsPA promoter)
This part was designed with the idea of improving the expression of AOX1A in response to a cold shock. To do that we combined the CsPA promoter (Part:BBa_K328000) with the OmpA + AOX1A previusly ensambled by iGEM10_GeorgiaTech.
As the part K328000 is a basic part, instead of a combination of subparts (Promoter, etc...), we had to analyse the sequence, cut out the HybB promoter and combine manually with the CsPA sequence.
Combination of two existing biobricks from the parts registry
Nitrate and Nitrite sensitive promoter PyeaR with a GFP coding device and strong RBS to create a nitrate-sensitive system which signals through expression of GFP. Optimum use appears to be when sensing nitrate within a range of 0 - 20 mM
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
RBS.1 (strong) -- modified from R. Weiss
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
GFP (mut3b) [note that this part does not have a barcode]
PyeaR promoter, responsive to nitrate, nitrite and nitric oxide
BioBricked from E. coli K-12 genomic DNA by the Edinburgh 2009 iGEM team.
PyeaR promoter. This is the promoter of the Escherichia coli yeaR/yoaG operon (see Lin, H.-Y., Bledsoe, P.J., and Stewart, V. 2007. Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen-responsive regulator Fnr in ''Escherichia coli'' K-12. J. Bacteriol. 189, 7539-7548). Unlike other E. coli promoters responding to nitrate and nitrite, this promoter is not repressed under aerobic conditions.
Conversion of the iGEM parts registry to SBOL2.1