BBa_K672002
type: ComponentDefinition
persistent identity: BBa K672002
display id: BBa_K672002
version: 1
was generated by: iGEM to SBOL conversion
description: Nitrate sensing at low temperatures
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:13:03Z
mutable provenance: http://partsregistry.org/wiki/index.php?title=Part:BBa_K672001 (New Promoter For Periplasmic Heat Generation) http://partsregistry.org/Part:BBa_K381001 (Nitrate reporter: PyeaR - GFP composite)
owned by: james
owned by: myers
mutable description: This part consists of the combination of our part BBa_K672001 + the part BBa_K381001 which was designed by iGEM10_BCCS-Bristol. It's purpose is to combine the nitrate sensing capabilities of Bristol's part with our heat production at temperatures of aproximately 15??C, which leads to have a bacteria that survives and senses nitrate at low temperatures.
discontinued: false
dominant: false
experience: experience/None
group u list: _855_
m user id: 0
owner id: 10335
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Emmanuel Garuz
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K381001
component: BBa_K672001
sequence annotation: BBa_K672001
sequence annotation: BBa_K381001
sequence: https://synbiohub.org/public/igem/BBa_K672002_sequence/1

BBa_K381001
type: Component
persistent identity: component2224526
display id: component2224526
version: 1
top level: BBa_K672002
definition: BBa_K381001
access: public

BBa_K672001
type: Component
persistent identity: component2224509
display id: component2224509
version: 1
top level: BBa_K672002
definition: BBa_K672001
access: public

BBa_K672001
type: SequenceAnnotation
persistent identity: annotation2224509
display id: annotation2224509
version: 1
top level: BBa_K672002
location: https://synbiohub.org/public/igem/BBa_K672002/annotation2224509/range2224509/1
component: BBa_K672001

https://synbiohub.org/public/igem/BBa_K672002/annotation2224509/range2224509/1
type: Range
persistent identity: range2224509
display id: range2224509
version: 1
top level: BBa_K672002
direction: direction/reverse
start: 1
end: 1445
orientation: inline

BBa_K381001
type: SequenceAnnotation
persistent identity: annotation2224526
display id: annotation2224526
version: 1
top level: BBa_K672002
location: https://synbiohub.org/public/igem/BBa_K672002/annotation2224526/range2224526/1
component: BBa_K381001

https://synbiohub.org/public/igem/BBa_K672002/annotation2224526/range2224526/1
type: Range
persistent identity: range2224526
display id: range2224526
version: 1
top level: BBa_K672002
direction: direction/reverse
start: 1454
end: 2439
orientation: inline

BBa_K672001
type: ComponentDefinition
persistent identity: BBa K672001
display id: BBa_K672001
version: 1
was generated by: iGEM to SBOL conversion
description: New Promoter For Periplasmic Heat Generation
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:13:03Z
mutable provenance: http://partsregistry.org/Part:BBa_K410000 (hybB-ompA-AOX1A) http://partsregistry.org/Part:BBa_K328000 (CsPA promoter)
owned by: james
owned by: myers
mutable description: This part was designed with the idea of improving the expression of AOX1A in response to a cold shock. To do that we combined the CsPA promoter (Part:BBa_K328000) with the OmpA + AOX1A previusly ensambled by iGEM10_GeorgiaTech.
discontinued: false
dominant: false
experience: experience/None
group u list: _855_
m user id: 0
owner id: 10335
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: As the part K328000 is a basic part, instead of a combination of subparts (Promoter, etc...), we had to analyse the sequence, cut out the HybB promoter and combine manually with the CsPA sequence.
star: false
creator: Emmanuel Garuz
type: DnaRegion
role: SO:0000804
role: partType/Device
sequence annotation: CsPA+ColdBox+UTR+SD+DB
sequence annotation: Notl Site
sequence annotation: RBS
sequence annotation: OmpA
sequence annotation: Xmal Site
sequence annotation: Alternative Oxidase
sequence: https://synbiohub.org/public/igem/BBa_K672001_sequence/1

CsPA+ColdBox+UTR+SD+DB
type: SequenceAnnotation
persistent identity: annotation2145567
display id: annotation2145567
version: 1
top level: BBa_K672001
location: https://synbiohub.org/public/igem/BBa_K672001/annotation2145567/range2145567/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K672001/annotation2145567/range2145567/1
type: Range
persistent identity: range2145567
display id: range2145567
version: 1
top level: BBa_K672001
direction: direction/forward
start: 1
end: 321
orientation: inline

Notl Site
type: SequenceAnnotation
persistent identity: annotation2145568
display id: annotation2145568
version: 1
top level: BBa_K672001
location: https://synbiohub.org/public/igem/BBa_K672001/annotation2145568/range2145568/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K672001/annotation2145568/range2145568/1
type: Range
persistent identity: range2145568
display id: range2145568
version: 1
top level: BBa_K672001
direction: direction/forward
start: 322
end: 329
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2145572
display id: annotation2145572
version: 1
top level: BBa_K672001
location: https://synbiohub.org/public/igem/BBa_K672001/annotation2145572/range2145572/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K672001/annotation2145572/range2145572/1
type: Range
persistent identity: range2145572
display id: range2145572
version: 1
top level: BBa_K672001
direction: direction/forward
start: 330
end: 335
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation2145573
display id: annotation2145573
version: 1
top level: BBa_K672001
location: https://synbiohub.org/public/igem/BBa_K672001/annotation2145573/range2145573/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K672001/annotation2145573/range2145573/1
type: Range
persistent identity: range2145573
display id: range2145573
version: 1
top level: BBa_K672001
direction: direction/forward
start: 342
end: 404
orientation: inline

Xmal Site
type: SequenceAnnotation
persistent identity: annotation2145575
display id: annotation2145575
version: 1
top level: BBa_K672001
location: https://synbiohub.org/public/igem/BBa_K672001/annotation2145575/range2145575/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K672001/annotation2145575/range2145575/1
type: Range
persistent identity: range2145575
display id: range2145575
version: 1
top level: BBa_K672001
direction: direction/forward
start: 405
end: 410
orientation: inline

Alternative Oxidase
type: SequenceAnnotation
persistent identity: annotation2145592
display id: annotation2145592
version: 1
top level: BBa_K672001
location: https://synbiohub.org/public/igem/BBa_K672001/annotation2145592/range2145592/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K672001/annotation2145592/range2145592/1
type: Range
persistent identity: range2145592
display id: range2145592
version: 1
top level: BBa_K672001
direction: direction/forward
start: 411
end: 1445
orientation: inline

BBa_K381001
type: ComponentDefinition
persistent identity: BBa K381001
display id: BBa_K381001
version: 1
was generated by: iGEM to SBOL conversion
description: Nitrate reporter: PyeaR - GFP composite
created: 2010-10-04T11:00:00Z
modified: 2015-05-08T01:12:17Z
mutable provenance: Combination of two existing biobricks from the parts registry
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Nitrate and Nitrite sensitive promoter PyeaR with a GFP coding device and strong RBS to create a nitrate-sensitive system which signals through expression of GFP. Optimum use appears to be when sensing nitrate within a range of 0 - 20 mM
discontinued: false
dominant: false
experience: experience/Works
group u list: _487_
m user id: 0
owner id: 6940
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None
star: false
creator: Katharine Coyte
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0030
component: BBa_K216005
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_K216005
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K381001_sequence/1

BBa_B0030
type: Component
persistent identity: component2082866
display id: component2082866
version: 1
top level: BBa_K381001
definition: BBa_B0030
access: public

BBa_K216005
type: Component
persistent identity: component2082864
display id: component2082864
version: 1
top level: BBa_K381001
definition: BBa_K216005
access: public

BBa_B0010
type: Component
persistent identity: component2082870
display id: component2082870
version: 1
top level: BBa_K381001
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component2082869
display id: component2082869
version: 1
top level: BBa_K381001
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component2082872
display id: component2082872
version: 1
top level: BBa_K381001
definition: BBa_B0012
access: public

BBa_K216005
type: SequenceAnnotation
persistent identity: annotation2082864
display id: annotation2082864
version: 1
top level: BBa_K381001
location: https://synbiohub.org/public/igem/BBa_K381001/annotation2082864/range2082864/1
component: BBa_K216005

https://synbiohub.org/public/igem/BBa_K381001/annotation2082864/range2082864/1
type: Range
persistent identity: range2082864
display id: range2082864
version: 1
top level: BBa_K381001
direction: direction/reverse
start: 1
end: 100
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2082866
display id: annotation2082866
version: 1
top level: BBa_K381001
location: https://synbiohub.org/public/igem/BBa_K381001/annotation2082866/range2082866/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K381001/annotation2082866/range2082866/1
type: Range
persistent identity: range2082866
display id: range2082866
version: 1
top level: BBa_K381001
direction: direction/forward
start: 109
end: 123
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2082869
display id: annotation2082869
version: 1
top level: BBa_K381001
location: https://synbiohub.org/public/igem/BBa_K381001/annotation2082869/range2082869/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K381001/annotation2082869/range2082869/1
type: Range
persistent identity: range2082869
display id: range2082869
version: 1
top level: BBa_K381001
direction: direction/reverse
start: 130
end: 849
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2082870
display id: annotation2082870
version: 1
top level: BBa_K381001
location: https://synbiohub.org/public/igem/BBa_K381001/annotation2082870/range2082870/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K381001/annotation2082870/range2082870/1
type: Range
persistent identity: range2082870
display id: range2082870
version: 1
top level: BBa_K381001
direction: direction/forward
start: 858
end: 937
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2082872
display id: annotation2082872
version: 1
top level: BBa_K381001
location: https://synbiohub.org/public/igem/BBa_K381001/annotation2082872/range2082872/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K381001/annotation2082872/range2082872/1
type: Range
persistent identity: range2082872
display id: range2082872
version: 1
top level: BBa_K381001
direction: direction/forward
start: 946
end: 986
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K216005
type: ComponentDefinition
persistent identity: BBa K216005
display id: BBa_K216005
version: 1
was generated by: iGEM to SBOL conversion
description: PyeaR promoter, responsive to nitrate, nitrite and nitric oxide
created: 2009-09-24T11:00:00Z
modified: 2015-05-08T01:11:30Z
mutable provenance: BioBricked from E. coli K-12 genomic DNA by the Edinburgh 2009 iGEM team.
owned by: james
owned by: myers
mutable description: PyeaR promoter. This is the promoter of the Escherichia coli yeaR/yoaG operon (see Lin, H.-Y., Bledsoe, P.J., and Stewart, V. 2007. Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen-responsive regulator Fnr in ''Escherichia coli'' K-12. J. Bacteriol. 189, 7539-7548). Unlike other E. coli promoters responding to nitrate and nitrite, this promoter is not repressed under aerobic conditions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: No special considerations.
star: false
creator: Edinburgh iGEM 2009
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: NarL-binding region
sequence annotation: NsrR-binding region
sequence annotation: promoter -35 region
sequence annotation: promoter -10 region
sequence: https://synbiohub.org/public/igem/BBa_K216005_sequence/1

NarL-binding region
type: SequenceAnnotation
persistent identity: annotation2027072
display id: annotation2027072
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027072/range2027072/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K216005/annotation2027072/range2027072/1
type: Range
persistent identity: range2027072
display id: range2027072
version: 1
top level: BBa_K216005
direction: direction/forward
start: 50
end: 65
orientation: inline

NsrR-binding region
type: SequenceAnnotation
persistent identity: annotation2027073
display id: annotation2027073
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027073/range2027073/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K216005/annotation2027073/range2027073/1
type: Range
persistent identity: range2027073
display id: range2027073
version: 1
top level: BBa_K216005
direction: direction/forward
start: 58
end: 80
orientation: inline

promoter -35 region
type: SequenceAnnotation
persistent identity: annotation2027074
display id: annotation2027074
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027074/range2027074/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K216005/annotation2027074/range2027074/1
type: Range
persistent identity: range2027074
display id: range2027074
version: 1
top level: BBa_K216005
direction: direction/forward
start: 66
end: 71
orientation: inline

promoter -10 region
type: SequenceAnnotation
persistent identity: annotation2027075
display id: annotation2027075
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027075/range2027075/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K216005/annotation2027075/range2027075/1
type: Range
persistent identity: range2027075
display id: range2027075
version: 1
top level: BBa_K216005
direction: direction/forward
start: 89
end: 94
orientation: inline

https://synbiohub.org/public/igem/BBa_K672002_sequence/1
type: Sequence
persistent identity: BBa K672002 sequence
display id: BBa_K672002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cggcattaagtaagcagttgatggaatagacttttatccactttattgctgtttacggtcctgatgacaggaccgttttccaaccgattaatcataaatatgaaaaataattgttgcatcacccgccaatgcgtggcttaatgcacatcaacggtttgacgtacagaccattaaagcagtgtagtaaggcaagtcccttcaagagttatcgttgatacccctcgtagtgcacattcctttaacgcttcaaaatctgtaaagcacgccatatcgccgaaaggcacacttaattattaaaggtaatacactatgtccggtaaagcggccgcaggagatatcatatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgttgcgcaagctcccgggatgatgaacagcaaactggcagctctgctgctgaaacaactgggttctgctaccgttcgtaccgtgacaatgggtcctctgaatgggattaccactgagtcctcgtgctttctgcatgcatctggtccggtcgttccaggtcgccgtacttggattcgcttttcttgtctgggagtccgtaatggttctaccagtgccctgaataacaaagagaaagaggagaaaggcgtccgtacaagttctactgttggtggcgccaatcgccctgaggataaaatgatcgtgtcctattggggtatgcctccggcaaatctgactaaaaaagacggctccgagtggaaatggaactcattccgtccatgggaaacctataaagccgatctgtcgatcgatctgaaaaaacaccatagcccggtgacgtttatggataaactggcctattggaccgttaaagccctgcgctatccgactgatattctgttccaaaaccgttatggctgtcgtgccatgatgctggagactgtggcggcagtaccagggatggttggggggatgctgctgcacctgaaatctctgcgtcgcttcgaacattctggtgggtggatcaaaacactgctggaagaagccgaaaacgagcgtatgcacctgatgaccttcatggaagtgtctcagccaaaatggtatgaacgtgccctggttgtagcagttcagggtgtgttcttcaacacgtattttctgggttatctgatcagccctcgcttcgctcatcgtgtggttggatatctggaggaggaagcaattcactcctataccgagtttctgaaagaactggacaaaggcaacatccagaacgtcccagcaccagcaattgccgtggattattggcaactgccaccggattctacgctgcgtgatgtggtgatggtcgtccgtgctgatgaagcacatcatcgtgacgtgaaccactttgccagcgacattcacgaccaaggctatgagctgaaagagtctcctgctcctctgggatatcattaatactagagttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacgtactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacaca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K672001_sequence/1
type: Sequence
persistent identity: BBa K672001 sequence
display id: BBa_K672001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cggcattaagtaagcagttgatggaatagacttttatccactttattgctgtttacggtcctgatgacaggaccgttttccaaccgattaatcataaatatgaaaaataattgttgcatcacccgccaatgcgtggcttaatgcacatcaacggtttgacgtacagaccattaaagcagtgtagtaaggcaagtcccttcaagagttatcgttgatacccctcgtagtgcacattcctttaacgcttcaaaatctgtaaagcacgccatatcgccgaaaggcacacttaattattaaaggtaatacactatgtccggtaaagcggccgcaggagatatcatatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgttgcgcaagctcccgggatgatgaacagcaaactggcagctctgctgctgaaacaactgggttctgctaccgttcgtaccgtgacaatgggtcctctgaatgggattaccactgagtcctcgtgctttctgcatgcatctggtccggtcgttccaggtcgccgtacttggattcgcttttcttgtctgggagtccgtaatggttctaccagtgccctgaataacaaagagaaagaggagaaaggcgtccgtacaagttctactgttggtggcgccaatcgccctgaggataaaatgatcgtgtcctattggggtatgcctccggcaaatctgactaaaaaagacggctccgagtggaaatggaactcattccgtccatgggaaacctataaagccgatctgtcgatcgatctgaaaaaacaccatagcccggtgacgtttatggataaactggcctattggaccgttaaagccctgcgctatccgactgatattctgttccaaaaccgttatggctgtcgtgccatgatgctggagactgtggcggcagtaccagggatggttggggggatgctgctgcacctgaaatctctgcgtcgcttcgaacattctggtgggtggatcaaaacactgctggaagaagccgaaaacgagcgtatgcacctgatgaccttcatggaagtgtctcagccaaaatggtatgaacgtgccctggttgtagcagttcagggtgtgttcttcaacacgtattttctgggttatctgatcagccctcgcttcgctcatcgtgtggttggatatctggaggaggaagcaattcactcctataccgagtttctgaaagaactggacaaaggcaacatccagaacgtcccagcaccagcaattgccgtggattattggcaactgccaccggattctacgctgcgtgatgtggtgatggtcgtccgtgctgatgaagcacatcatcgtgacgtgaaccactttgccagcgacattcacgaccaaggctatgagctgaaagagtctcctgctcctctgggatatcattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K381001_sequence/1
type: Sequence
persistent identity: BBa K381001 sequence
display id: BBa_K381001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacgtactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K216005_sequence/1
type: Sequence
persistent identity: BBa K216005 sequence
display id: BBa_K216005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z