LOCUS       BBa_K675001             2196 bp    DNA     linear       13-Nov-2024
DEFINITION  Arsenic Detector
ACCESSION   BBa_K675001
VERSION     BBa_K675001.1  
FEATURES             Location/Qualifiers
     misc_feature    9..41
                     /label=ArsR binding site
     promoter        38..72
                     /label=-35 and -10 region
     promoter        1..72
                     /label=BBa_K190015
     RBS             81..93
                     /label=RBS-3\Weak
     RBS             87..90
                     /label=RBS
     misc_feature    81..93
                     /label=BBa_B0032
     RBS             81..93
                     /label=BBa_B0032
     CDS             100..1173
                     /label=BMST1
     misc_feature    100..102
                     /label=start codon
     misc_feature    1171..1173
                     /label=stop codon
     CDS             100..1173
                     /label=BBa_J45004
     stem_loop       1193..1236
                     /label=stem_loop
     misc_feature    1182..1261
                     /label=BBa_B0010
     terminator      1182..1261
                     /label=BBa_B0010
     stem_loop       1277..1296
                     /label=T7 TE
     misc_feature    1303..1303
                     /label=stop
     polyA_site      1297..1310
                     /label=polya
     misc_feature    1270..1310
                     /label=BBa_B0012
     terminator      1270..1310
                     /label=BBa_B0012
     misc_feature    81..1310
                     /label=BBa_J45119
     RBS             1319..1333
                     /label=RBS-1\Strong
     RBS             1326..1330
                     /label=RBS
     misc_feature    1319..1333
                     /label=BBa_B0030
     RBS             1319..1333
                     /label=BBa_B0030
     CDS             1340..2059
                     /label=GFP protein
     CDS             1340..2059
                     /label=BBa_E0040
     stem_loop       2079..2122
                     /label=stem_loop
     misc_feature    2068..2147
                     /label=BBa_B0010
     terminator      2068..2147
                     /label=BBa_B0010
     stem_loop       2163..2182
                     /label=T7 TE
     misc_feature    2189..2189
                     /label=stop
     polyA_site      2183..2196
                     /label=polya
     misc_feature    2156..2196
                     /label=BBa_B0012
     terminator      2156..2196
                     /label=BBa_B0012
     misc_feature    1319..2196
                     /label=BBa_E0840
ORIGIN
        1 ctgcacttac acattcgtta agtcatatat gtttttgact tatccgcttc gaagagagac
       61 actacctgca attactagag tcacacagga aagtactaga tggaagttgt tgaagttctt
      121 cacatgaatg gaggaaatgg agacagtagc tatgcaaaca attctttggt tcagcaaaag
      181 gtgattctca tgacaaagcc aataactgag caagccatga ttgatctcta cagcagcctc
      241 tttccagaaa ccttatgcat tgcagatttg ggttgttctt tgggagctaa cactttcttg
      301 gtggtctcac agcttgttaa aatagtagaa aaagaacgaa aaaagcatgg ttttaagtct
      361 ccagagtttt attttcactt caatgatctt cctggcaatg attttaatac actttttcag
      421 tcactggggg catttcaaga agatttgaga aagcatatag gggaaagctt tggtccatgt
      481 tttttcagtg gagtgcctgg ttcattttat actagacttt tcccttccaa aagtttacat
      541 tttgtttact cctcctacag tctcatgtgg ctatctcagg tgcctaatgg gattgaaaat
      601 aacaagggaa acatttacat ggcaagaaca agccctctaa gtgttattaa agcatactac
      661 aagcaatatg aaatagattt ttcaaatttt ctcaagtacc gttcagagga attgatgaaa
      721 ggtggaaaga tggtgttaac actcctaggt agagaaagtg aggatcctac tagcaaagaa
      781 tgctgttaca tttgggagct tctagccatg gccctcaata agttggttga agagggattg
      841 ataaaagaag agaaagtaga tgcattcaat attcctcaat acacaccatc accagcagaa
      901 gtaaagtaca tagttgagaa ggaaggatca ttcaccatta atcgcttgga aacatcaaga
      961 gttcattgga atgcttctaa taatgagaag aatggtggtt acaatgtgtc aaggtgcatg
     1021 agagctgtgg ctgagccttt gcttgtcagc cactttgaca aggaattgat ggatttagtg
     1081 ttccacaagt acgaagagat tgtttctgat tgcatgtcca aagagaatac tgagtttata
     1141 aatgtcatca tctccttgac caaaataaat taatactaga gccaggcatc aaataaaacg
     1201 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1261 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagat
     1321 taaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
     1381 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     1441 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     1501 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
     1561 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1621 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     1681 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     1741 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1801 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     1861 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     1921 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     1981 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     2041 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     2101 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     2161 ctggctcacc ttcgggtggg cctttctgcg tttata
//