##gff-version 3
##sequence-region BBa_K675001 1 2196
BBa_K675001	.	promoter	1	72	.	+	0	ID=BBa_K190015;Name=BBa_K190015
BBa_K675001	.	promoter	38	72	.	+	0	ID=annotation2011502;Name=-35 and -10 region;Parent=BBa_K190015
BBa_K675001	.	non_covalent_binding_site	9	41	.	+	0	ID=annotation2011501;Name=ArsR binding site;Parent=BBa_K190015
BBa_K675001	.	engineered_region	1319	2196	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K675001	.	terminator	2068	2147	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K675001	.	stem_loop	2079	2122	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K675001	.	engineered_region	2068	2147	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K675001	.	ribosome_entry_site	1319	1333	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K675001	.	ribosome_entry_site	1319	1333	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K675001	.	engineered_region	1319	1333	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K675001	.	ribosome_entry_site	1326	1330	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K675001	.	terminator	2156	2196	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K675001	.	stem_loop	2163	2182	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K675001	.	stop_codon	2189	2189	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K675001	.	polyA_site	2183	2196	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K675001	.	engineered_region	2156	2196	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K675001	.	CDS	1340	2059	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K675001	.	CDS	1340	2059	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K675001	.	engineered_region	81	1310	.	+	0	ID=BBa_J45119;Name=BBa_J45119
BBa_K675001	.	terminator	1270	1310	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45119
BBa_K675001	.	stem_loop	1277	1296	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K675001	.	stop_codon	1303	1303	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K675001	.	polyA_site	1297	1310	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K675001	.	engineered_region	1270	1310	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K675001	.	ribosome_entry_site	81	93	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J45119
BBa_K675001	.	ribosome_entry_site	87	90	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K675001	.	ribosome_entry_site	81	93	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K675001	.	engineered_region	81	93	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K675001	.	terminator	1182	1261	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45119
BBa_K675001	.	stem_loop	1193	1236	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K675001	.	engineered_region	1182	1261	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K675001	.	CDS	100	1173	.	+	0	ID=BBa_J45004;Name=BBa_J45004;Parent=BBa_J45119
BBa_K675001	.	start_codon	100	102	.	+	0	ID=annotation1880519;Name=start codon;Parent=BBa_J45004
BBa_K675001	.	stop_codon	1171	1173	.	+	0	ID=annotation1880520;Name=stop codon;Parent=BBa_J45004
BBa_K675001	.	CDS	100	1173	.	+	0	ID=annotation1880517;Name=BMST1;Parent=BBa_J45004
>BBa_K675001
ctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactacctgcaattactaga
gtcacacaggaaagtactagatggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaa
caattctttggttcagcaaaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagc
ctctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttg
ttaaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctgg
caatgattttaatacactttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggt
ccatgttttttcagtggagtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcct
cctacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaag
ccctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaa
ttgatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttaca
tttgggagcttctagccatggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaa
tattcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttg
gaaacatcaagagttcattggaatgcttctaataatgagaagaatggtggttacaatgtgtcaaggtgcatgagagctg
tggctgagcctttgcttgtcagccactttgacaaggaattgatggatttagtgttccacaagtacgaagagattgtttc
tgattgcatgtccaaagagaatactgagtttataaatgtcatcatctccttgaccaaaataaattaatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagattaaagaggagaaatactagatgc
gtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttc
tgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacct
gttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagc
atgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaa
gacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagat
ggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatg
gaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatac
tccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaac
gaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaat
aataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata