LOCUS BBa_K675003 2276 bp DNA linear 26-Dec-2024 DEFINITION Arsenic Detector ACCESSION BBa_K675003 VERSION BBa_K675003.1 FEATURES Location/Qualifiers misc_feature 9..41 /label=ArsR binding site promoter 38..72 /label=-35 and -10 region promoter 1..72 /label=BBa_K190015 RBS 81..93 /label=RBS-3\Weak RBS 87..90 /label=RBS misc_feature 81..93 /label=BBa_B0032 RBS 81..93 /label=BBa_B0032 CDS 100..1173 /label=BMST1 misc_feature 100..102 /label=start codon misc_feature 1171..1173 /label=stop codon CDS 100..1173 /label=BBa_J45004 stem_loop 1193..1236 /label=stem_loop misc_feature 1182..1261 /label=BBa_B0010 terminator 1182..1261 /label=BBa_B0010 stem_loop 1277..1296 /label=T7 TE misc_feature 1303..1303 /label=stop polyA_site 1297..1310 /label=polya misc_feature 1270..1310 /label=BBa_B0012 terminator 1270..1310 /label=BBa_B0012 misc_feature 81..1310 /label=BBa_J45119 misc_feature 1327..1359 /label=ArsR binding site promoter 1356..1390 /label=-35 and -10 region promoter 1319..1390 /label=BBa_K190015 RBS 1399..1413 /label=RBS-1\Strong RBS 1406..1410 /label=RBS misc_feature 1399..1413 /label=BBa_B0030 RBS 1399..1413 /label=BBa_B0030 CDS 1420..2139 /label=GFP protein CDS 1420..2139 /label=BBa_E0040 stem_loop 2159..2202 /label=stem_loop misc_feature 2148..2227 /label=BBa_B0010 terminator 2148..2227 /label=BBa_B0010 stem_loop 2243..2262 /label=T7 TE misc_feature 2269..2269 /label=stop polyA_site 2263..2276 /label=polya misc_feature 2236..2276 /label=BBa_B0012 terminator 2236..2276 /label=BBa_B0012 misc_feature 1399..2276 /label=BBa_E0840 ORIGIN 1 ctgcacttac acattcgtta agtcatatat gtttttgact tatccgcttc gaagagagac 61 actacctgca attactagag tcacacagga aagtactaga tggaagttgt tgaagttctt 121 cacatgaatg gaggaaatgg agacagtagc tatgcaaaca attctttggt tcagcaaaag 181 gtgattctca tgacaaagcc aataactgag caagccatga ttgatctcta cagcagcctc 241 tttccagaaa ccttatgcat tgcagatttg ggttgttctt tgggagctaa cactttcttg 301 gtggtctcac agcttgttaa aatagtagaa aaagaacgaa aaaagcatgg ttttaagtct 361 ccagagtttt attttcactt caatgatctt cctggcaatg attttaatac actttttcag 421 tcactggggg catttcaaga agatttgaga aagcatatag gggaaagctt tggtccatgt 481 tttttcagtg gagtgcctgg ttcattttat actagacttt tcccttccaa aagtttacat 541 tttgtttact cctcctacag tctcatgtgg ctatctcagg tgcctaatgg gattgaaaat 601 aacaagggaa acatttacat ggcaagaaca agccctctaa gtgttattaa agcatactac 661 aagcaatatg aaatagattt ttcaaatttt ctcaagtacc gttcagagga attgatgaaa 721 ggtggaaaga tggtgttaac actcctaggt agagaaagtg aggatcctac tagcaaagaa 781 tgctgttaca tttgggagct tctagccatg gccctcaata agttggttga agagggattg 841 ataaaagaag agaaagtaga tgcattcaat attcctcaat acacaccatc accagcagaa 901 gtaaagtaca tagttgagaa ggaaggatca ttcaccatta atcgcttgga aacatcaaga 961 gttcattgga atgcttctaa taatgagaag aatggtggtt acaatgtgtc aaggtgcatg 1021 agagctgtgg ctgagccttt gcttgtcagc cactttgaca aggaattgat ggatttagtg 1081 ttccacaagt acgaagagat tgtttctgat tgcatgtcca aagagaatac tgagtttata 1141 aatgtcatca tctccttgac caaaataaat taatactaga gccaggcatc aaataaaacg 1201 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1261 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagct 1321 gcacttacac attcgttaag tcatatatgt ttttgactta tccgcttcga agagagacac 1381 tacctgcaat tactagagat taaagaggag aaatactaga tgcgtaaagg agaagaactt 1441 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt 1501 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt 1561 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt 1621 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc 1681 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag 1741 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt 1801 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca 1861 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt 1921 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca 1981 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt 2041 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct 2101 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat 2161 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2221 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata // LOCUS BBa_K675002 2276 bp DNA linear 26-Dec-2024 DEFINITION Arsenic Detector ACCESSION BBa_K675002 VERSION BBa_K675002.1 FEATURES Location/Qualifiers misc_feature 9..41 /label=ArsR binding site promoter 38..72 /label=-35 and -10 region promoter 1..72 /label=BBa_K190015 RBS 81..93 /label=RBS-3\Weak RBS 87..90 /label=RBS misc_feature 81..93 /label=BBa_B0032 RBS 81..93 /label=BBa_B0032 CDS 100..1173 /label=BMST1 misc_feature 100..102 /label=start codon misc_feature 1171..1173 /label=stop codon CDS 100..1173 /label=BBa_J45004 stem_loop 1193..1236 /label=stem_loop misc_feature 1182..1261 /label=BBa_B0010 terminator 1182..1261 /label=BBa_B0010 stem_loop 1277..1296 /label=T7 TE misc_feature 1303..1303 /label=stop polyA_site 1297..1310 /label=polya misc_feature 1270..1310 /label=BBa_B0012 terminator 1270..1310 /label=BBa_B0012 misc_feature 81..1310 /label=BBa_J45119 misc_feature 1327..1359 /label=ArsR binding site promoter 1356..1390 /label=-35 and -10 region promoter 1319..1390 /label=BBa_K190015 RBS 1399..1413 /label=RBS-1\Strong RBS 1406..1410 /label=RBS misc_feature 1399..1413 /label=BBa_B0030 RBS 1399..1413 /label=BBa_B0030 CDS 1420..2139 /label=GFP protein CDS 1420..2139 /label=BBa_E0040 stem_loop 2159..2202 /label=stem_loop misc_feature 2148..2227 /label=BBa_B0010 terminator 2148..2227 /label=BBa_B0010 stem_loop 2243..2262 /label=T7 TE misc_feature 2269..2269 /label=stop polyA_site 2263..2276 /label=polya misc_feature 2236..2276 /label=BBa_B0012 terminator 2236..2276 /label=BBa_B0012 misc_feature 1399..2276 /label=BBa_E0840 ORIGIN 1 ctgcacttac acattcgtta agtcatatat gtttttgact tatccgcttc gaagagagac 61 actacctgca attactagag tcacacagga aagtactaga tggaagttgt tgaagttctt 121 cacatgaatg gaggaaatgg agacagtagc tatgcaaaca attctttggt tcagcaaaag 181 gtgattctca tgacaaagcc aataactgag caagccatga ttgatctcta cagcagcctc 241 tttccagaaa ccttatgcat tgcagatttg ggttgttctt tgggagctaa cactttcttg 301 gtggtctcac agcttgttaa aatagtagaa aaagaacgaa aaaagcatgg ttttaagtct 361 ccagagtttt attttcactt caatgatctt cctggcaatg attttaatac actttttcag 421 tcactggggg catttcaaga agatttgaga aagcatatag gggaaagctt tggtccatgt 481 tttttcagtg gagtgcctgg ttcattttat actagacttt tcccttccaa aagtttacat 541 tttgtttact cctcctacag tctcatgtgg ctatctcagg tgcctaatgg gattgaaaat 601 aacaagggaa acatttacat ggcaagaaca agccctctaa gtgttattaa agcatactac 661 aagcaatatg aaatagattt ttcaaatttt ctcaagtacc gttcagagga attgatgaaa 721 ggtggaaaga tggtgttaac actcctaggt agagaaagtg aggatcctac tagcaaagaa 781 tgctgttaca tttgggagct tctagccatg gccctcaata agttggttga agagggattg 841 ataaaagaag agaaagtaga tgcattcaat attcctcaat acacaccatc accagcagaa 901 gtaaagtaca tagttgagaa ggaaggatca ttcaccatta atcgcttgga aacatcaaga 961 gttcattgga atgcttctaa taatgagaag aatggtggtt acaatgtgtc aaggtgcatg 1021 agagctgtgg ctgagccttt gcttgtcagc cactttgaca aggaattgat ggatttagtg 1081 ttccacaagt acgaagagat tgtttctgat tgcatgtcca aagagaatac tgagtttata 1141 aatgtcatca tctccttgac caaaataaat taatactaga gccaggcatc aaataaaacg 1201 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1261 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagct 1321 gcacttacac attcgttaag tcatatatgt ttttgactta tccgcttcga agagagacac 1381 tacctgcaat tactagagat taaagaggag aaatactaga tgcgtaaagg agaagaactt 1441 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt 1501 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt 1561 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt 1621 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc 1681 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag 1741 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt 1801 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca 1861 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt 1921 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca 1981 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt 2041 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct 2101 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat 2161 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2221 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //