LOCUS       BBa_K675003             2276 bp    DNA     linear       22-Dec-2024
DEFINITION  Arsenic Detector
ACCESSION   BBa_K675003
VERSION     BBa_K675003.1  
FEATURES             Location/Qualifiers
     misc_feature    9..41
                     /label=ArsR binding site
     promoter        38..72
                     /label=-35 and -10 region
     promoter        1..72
                     /label=BBa_K190015
     RBS             81..93
                     /label=RBS-3\Weak
     RBS             87..90
                     /label=RBS
     misc_feature    81..93
                     /label=BBa_B0032
     RBS             81..93
                     /label=BBa_B0032
     CDS             100..1173
                     /label=BMST1
     misc_feature    100..102
                     /label=start codon
     misc_feature    1171..1173
                     /label=stop codon
     CDS             100..1173
                     /label=BBa_J45004
     stem_loop       1193..1236
                     /label=stem_loop
     misc_feature    1182..1261
                     /label=BBa_B0010
     terminator      1182..1261
                     /label=BBa_B0010
     stem_loop       1277..1296
                     /label=T7 TE
     misc_feature    1303..1303
                     /label=stop
     polyA_site      1297..1310
                     /label=polya
     misc_feature    1270..1310
                     /label=BBa_B0012
     terminator      1270..1310
                     /label=BBa_B0012
     misc_feature    81..1310
                     /label=BBa_J45119
     misc_feature    1327..1359
                     /label=ArsR binding site
     promoter        1356..1390
                     /label=-35 and -10 region
     promoter        1319..1390
                     /label=BBa_K190015
     RBS             1399..1413
                     /label=RBS-1\Strong
     RBS             1406..1410
                     /label=RBS
     misc_feature    1399..1413
                     /label=BBa_B0030
     RBS             1399..1413
                     /label=BBa_B0030
     CDS             1420..2139
                     /label=GFP protein
     CDS             1420..2139
                     /label=BBa_E0040
     stem_loop       2159..2202
                     /label=stem_loop
     misc_feature    2148..2227
                     /label=BBa_B0010
     terminator      2148..2227
                     /label=BBa_B0010
     stem_loop       2243..2262
                     /label=T7 TE
     misc_feature    2269..2269
                     /label=stop
     polyA_site      2263..2276
                     /label=polya
     misc_feature    2236..2276
                     /label=BBa_B0012
     terminator      2236..2276
                     /label=BBa_B0012
     misc_feature    1399..2276
                     /label=BBa_E0840
ORIGIN
        1 ctgcacttac acattcgtta agtcatatat gtttttgact tatccgcttc gaagagagac
       61 actacctgca attactagag tcacacagga aagtactaga tggaagttgt tgaagttctt
      121 cacatgaatg gaggaaatgg agacagtagc tatgcaaaca attctttggt tcagcaaaag
      181 gtgattctca tgacaaagcc aataactgag caagccatga ttgatctcta cagcagcctc
      241 tttccagaaa ccttatgcat tgcagatttg ggttgttctt tgggagctaa cactttcttg
      301 gtggtctcac agcttgttaa aatagtagaa aaagaacgaa aaaagcatgg ttttaagtct
      361 ccagagtttt attttcactt caatgatctt cctggcaatg attttaatac actttttcag
      421 tcactggggg catttcaaga agatttgaga aagcatatag gggaaagctt tggtccatgt
      481 tttttcagtg gagtgcctgg ttcattttat actagacttt tcccttccaa aagtttacat
      541 tttgtttact cctcctacag tctcatgtgg ctatctcagg tgcctaatgg gattgaaaat
      601 aacaagggaa acatttacat ggcaagaaca agccctctaa gtgttattaa agcatactac
      661 aagcaatatg aaatagattt ttcaaatttt ctcaagtacc gttcagagga attgatgaaa
      721 ggtggaaaga tggtgttaac actcctaggt agagaaagtg aggatcctac tagcaaagaa
      781 tgctgttaca tttgggagct tctagccatg gccctcaata agttggttga agagggattg
      841 ataaaagaag agaaagtaga tgcattcaat attcctcaat acacaccatc accagcagaa
      901 gtaaagtaca tagttgagaa ggaaggatca ttcaccatta atcgcttgga aacatcaaga
      961 gttcattgga atgcttctaa taatgagaag aatggtggtt acaatgtgtc aaggtgcatg
     1021 agagctgtgg ctgagccttt gcttgtcagc cactttgaca aggaattgat ggatttagtg
     1081 ttccacaagt acgaagagat tgtttctgat tgcatgtcca aagagaatac tgagtttata
     1141 aatgtcatca tctccttgac caaaataaat taatactaga gccaggcatc aaataaaacg
     1201 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1261 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagct
     1321 gcacttacac attcgttaag tcatatatgt ttttgactta tccgcttcga agagagacac
     1381 tacctgcaat tactagagat taaagaggag aaatactaga tgcgtaaagg agaagaactt
     1441 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     1501 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     1561 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
     1621 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
     1681 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     1741 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     1801 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     1861 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
     1921 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     1981 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     2041 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     2101 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat
     2161 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2221 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//