LOCUS BBa_K676005 5406 bp DNA linear 23-Dec-2024 DEFINITION Extractery ACCESSION BBa_K676005 VERSION BBa_K676005.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23114 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..775 /label=cIts CDS 62..775 /label=BBa_K098997 misc_feature 1424..1429 /label=PstI site CDS 782..2482 /label=XylR CDS 782..2482 /label=BBa_I723017 stem_loop 2502..2545 /label=stem_loop misc_feature 2491..2570 /label=BBa_B0010 terminator 2491..2570 /label=BBa_B0010 stem_loop 2586..2605 /label=T7 TE misc_feature 2612..2612 /label=stop polyA_site 2606..2619 /label=polya misc_feature 2579..2619 /label=BBa_B0012 terminator 2579..2619 /label=BBa_B0012 terminator 2491..2619 /label=BBa_B0015 promoter 2665..2670 /label=-10 promoter 2642..2647 /label=-35 misc_feature 2652..2668 /label=OR1 misc_feature 2628..2644 /label=OR2 misc_feature 2628..2676 /label=BBa_R0051 promoter 2628..2676 /label=BBa_R0051 misc_feature 2689..2692 /label=conserved RBS 2685..2696 /label=BBa_B0034 CDS 2721..3215 /label=T4 Endolysin CDS 2705..3218 /label=BBa_K112806 stem_loop 3238..3281 /label=stem_loop misc_feature 3227..3306 /label=BBa_B0010 terminator 3227..3306 /label=BBa_B0010 stem_loop 3322..3341 /label=T7 TE misc_feature 3348..3348 /label=stop polyA_site 3342..3355 /label=polya misc_feature 3315..3355 /label=BBa_B0012 terminator 3315..3355 /label=BBa_B0012 terminator 3227..3355 /label=BBa_B0015 promoter 3364..3683 /label=Pu promoter 3364..3683 /label=BBa_I723020 misc_feature 3696..3699 /label=conserved RBS 3692..3703 /label=BBa_B0034 misc_feature 3710..3712 /label=start misc_feature 4583..4585 /label=1 CDS 3710..4585 /label=T5 Phage Exonuclease misc_feature 3935..3940 /label=RL misc_feature 3710..4585 /label=BBa_K676006 CDS 3710..4585 /label=BBa_K676006 CDS 4610..5266 /label=T4 holin CDS 4594..5269 /label=BBa_K112805 stem_loop 5289..5332 /label=stem_loop misc_feature 5278..5357 /label=BBa_B0010 terminator 5278..5357 /label=BBa_B0010 stem_loop 5373..5392 /label=T7 TE misc_feature 5399..5399 /label=stop polyA_site 5393..5406 /label=polya misc_feature 5366..5406 /label=BBa_B0012 terminator 5366..5406 /label=BBa_B0012 terminator 5278..5406 /label=BBa_B0015 ORIGIN 1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa 121 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa 181 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc 241 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc 301 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag 361 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag 421 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc 481 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag 541 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt 601 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg 661 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg 721 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gctgatacta 781 gatgtcgctt acatacaaac ccaagatgca gcatgaggat atgcaagacc ttagcagcca 841 gatccgtttc gttgccgccg aaggcaagat ctggttggga gagcagcgca tgctcgtaat 901 gcagctatct acgctggcca gcttccgtcg cgaaattatc agcttgatcg gcgtcgagcg 961 ggccaagggt ttcttcctgc ggttgggcta tcagtccggc ctgatggatg ccgagctggc 1021 acgcaagctg cggccggcca tgcgcgagga ggaggtgttc ctggctgggc ctcaattgta 1081 tgcgctcaag gggatggtca aagtacgctt gctgacaatg gatatcgcca tccgggacgg 1141 acgtttcaac gtggaggccg agtggattga ttcctttgaa gtggatatct gccgaactga 1201 gctgggcctg atgaatgagc ccgtctgctg gacggtgcta ggctatgcta gcggctatgg 1261 ttcggcattc atgggccgca gaatcatttt ccaggaaact agctgtcgcg ggtgcggtga 1321 cgataaatgc cttatcgtcg gcaagaccgc agaagagtgg ggcgatgtca gcagtttcga 1381 agcctacttc aaaagcgacc cgatcgtaga cgagcgctac gagctgcaga cccaggttgc 1441 caacctgcgc aaccgcctga agcagtacga tgggcagtat tacggcattg gccattcgcc 1501 agcctacaag cgcatctgtg agaccatcga caaggctgca cgcggcaggg tttcggtcct 1561 gctactgggt gagactgggg tgggcaagga ggtaatcgcg cgcagcgtgc atttgcgcag 1621 tgagcgcgca gagcaaccct tcgtcgcggt gaactgtgcg gcaattccgc cggatctgat 1681 cgagtcggaa ctgtttggtg tcgataaggg cgcctatacg ggcgcggtca atgcacgcgc 1741 tggacgtttt gaacgggcca acggcggcac catctttctt gatgaggtga tcgaattgac 1801 gccgagggcc caggccaccc tgctacgggt attgcaggaa ggagagctag agcgggtcgg 1861 cggcgaccgc acgcgaaagg tcgacgtgag gttaatcacc gcaacaaacg agaacctgga 1921 agaggcggtc aagatggggc gctttcgcgc agacctgttc tttcggctga atgtttttcc 1981 cgtgcatatc ccgccgttgc gcgagcgcgt ggaagatatc ccgctgctgg tcgagcattt 2041 tcttagaagg caccataagg aatacggtaa gaagactctt ggcctgtctg atcgagcgat 2101 ggaggcctgc ctccactacc aatggccagg caatatccgc gagctggaga acgcccttga 2161 gcgcggggtg attcttaccg agagcaacga aagcatcaat gtcgagtcgc tgttcccggg 2221 gttggcgacg gctaccgaag gcgacaggct atcgagcgag ggccggttgg aggaggagtc 2281 cggtgacagt tggtttaggc aaattatcga ccagggcgtc agcctcgaag atctcgaagc 2341 gggtttaatg cgcacggcca tggaccgttg tgggcagaat atctcacagg cggcgcggtt 2401 gctgggattg acccgcccgg caatggccta tcgacttaag aagcttgacc ccagcttatc 2461 tgtgaaagca atgggccgat agtactagag ccaggcatca aataaaacga aaggctcagt 2521 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 2581 acactggctc accttcgggt gggcctttct gcgtttatat actagagtaa caccgtgcgt 2641 gttgactatt ttacctctgg cggtgataat ggttgctact agagaaagag gagaaatact 2701 agagatactt aggaggtatt atgaatatat ttgaaatgtt acgtatagat gaaggtctta 2761 gacttaaaat ctataaagac acagaaggct attacactat tggcatcggt catttgctta 2821 caaaaagtcc atcacttaat gctgctaaat ctgaattaga taaagctatt gggcgtaatt 2881 gcaatggtgt aattacaaaa gatgaggctg aaaaactctt taatcaggat gttgatgctg 2941 ctgttcgcgg aatcctgaga aatgctaaat taaaaccggt ttatgattct cttgatgcgg 3001 ttcgtcgctg tgcattgatt aatatggttt tccaaatggg agaaaccggt gtggcaggat 3061 ttactaactc tttacgtatg cttcaacaaa aacgctggga tgaagcagca gttaacttag 3121 ctaaaagtag atggtataat caaacaccta atcgcgcaaa acgagtcatt acaacgttta 3181 gaactggcac ttgggacgcg tataaaaatc tataaagcta ctagagccag gcatcaaata 3241 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 3301 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 3361 gagcccggga aagcgcgatg aacctttttt atcgctgcct tgatcaaatc gacaggtggt 3421 tatgcgcgat tgatgatttg ctcaaataca gccagcgtgc tgtagatttt ctctcatacc 3481 ccccctttct tttttacaaa gaaaatcaat aatttagatg aaataagggg atcggtataa 3541 gcaatggcat ggcggttgct agctatacga gacttaaaat aaaaatagtg gtgacccttc 3601 aatgttgtat tttctcaact ctgttcagat tggttgcttt cgccatgtat atcctcaaag 3661 cgggccagcc gtagccgtta cgctactaga gaaagaggag aaatactaga tgagtaaatc 3721 ctggggaaaa tttattgaag aagaggaagc tgaaatggct tcccgtcgta atctaatgat 3781 tgtcgatgga actaacttag gctttcgctt caaacataac aatagtaaaa aaccatttgc 3841 ctcaagttat gtttcaacta ttcaatctct ggcaaaatcc tactctgcca gaactacgat 3901 tgttctaggt gataagggaa aatccgtatt tcgcttggaa catctaccag agtataaagg 3961 taatcgtgat gaaaagtacg cacaacgtac ggaagaggag aaagcgctag atgagcagtt 4021 ctttgagtat ttgaaggatg ctttcgagtt gtgtaaaact acattcccaa cttttaccat 4081 tcgtggtgta gaagcagacg atatggcagc ttatattgtt aagctcatcg ggcatcttta 4141 tgatcacgtt tggctaatat ctacagatgg tgactgggat actttattaa cggataaagt 4201 ttctcgtttt tctttcacaa cacgtcgtga gtatcatctt cgtgatatgt atgaacatca 4261 taatgttgat gatgttgagc agtttatctc cctgaaagca attatgggag atctaggaga 4321 taatattcgt ggtgttgaag gaataggagc aaaacgcgga tataatatta ttcgtgagtt 4381 tggtaacgta ctggatatta ttgatcagct tccactgcct ggaaagcaga aatatataca 4441 gaacctgaat gcatcggaag aactgctttt ccgaaacttg attctggttg atttacctac 4501 ctactgtgtg gatgctattg ctgctgtagg tcaagatgtg ttagataagt ttacaaaaga 4561 tattttggag attgcagaac aatgatacta gagcttaaaa ggagggtcta tggcagcacc 4621 tagaatatca ttttcgccct ctgatattct atttggtgtt ctcgatcgct tgttcaaaga 4681 taacgctacc gggaaggttc ttgcttcccg ggtagctgtc gtaattcttt tgtttataat 4741 ggcgattgtt tggtataggg gagatagttt ctttgagtac tataagcaat caaagtatga 4801 aacatacagt gaaattattg aaaaggaaag aactgcacgc tttgaatctg tcgccctgga 4861 acaactccag atagttcata tatcatctga ggcagacttt agtgcggtgt attctttccg 4921 ccctaaaaac ttaaactatt ttgttgatat tatagcatac gaaggaaaat taccttcaac 4981 aataagtgaa aaatcacttg gaggatatcc tgttgataaa actatggatg aatatacagt 5041 tcatttaaat ggacgtcatt attattccaa ctcaaaattt gcttttttac caactaaaaa 5101 gcctactccc gaaataaact acatgtacag ttgtccatat tttaatttgg ataatatcta 5161 tgctggaacg ataaccatgt actggtatag aaatgatcat ataagtaatg accgccttga 5221 atcaatatgt gctcaggcgg ccagaatatt aggaagggct aaataattat actagagcca 5281 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 5341 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 5401 tttata //