LOCUS       BBa_K676008             2991 bp    DNA     linear       05-Nov-2024
DEFINITION  GyrA cassette
ACCESSION   BBa_K676008
VERSION     BBa_K676008.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     misc_feature    2852..2854
                     /label=1
     CDS             227..2854
                     /label=Gyrase A
     misc_feature    227..2854
                     /label=BBa_K676007
     misc_feature    227..2854
                     /label=BBa_K676007
     stem_loop       2874..2917
                     /label=stem_loop
     misc_feature    2863..2942
                     /label=BBa_B0010
     terminator      2863..2942
                     /label=BBa_B0010
     stem_loop       2958..2977
                     /label=T7 TE
     misc_feature    2984..2984
                     /label=stop
     polyA_site      2978..2991
                     /label=polya
     misc_feature    2951..2991
                     /label=BBa_B0012
     terminator      2951..2991
                     /label=BBa_B0012
     terminator      2863..2991
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcgaccttgc
      241 gagagaaatt acaccggtca acattgagga agagctgaag agctcctatc tggattatgc
      301 gatgtcggtc attgttggcc gtgcgctgcc agatgtccga gatggcctga agccggtaca
      361 ccgtcgcgta ctttacgcca tgaacgtact aggcaatgac tggaacaaag cctataaaaa
      421 atctgcccgt gtcgttggtg acgtaatcgg taaataccat ccccatggtg actcggcggt
      481 ctatgacacg atcgtccgca tggcgcagcc attctcgctg cgttatatgc tggtagacgg
      541 tcagggtaac ttcggttcta tcgacggcga ctctgcggcg gcaatgcgtt atacggaaat
      601 ccgtctggcg aaaattgccc atgaactgat ggccgatctc gaaaaagaga cggtcgattt
      661 cgttgataac tatgacggca cggaaaaaat tccggacgtc atgccaacca aaattcctaa
      721 cctgctggtg aacggttctt ccggtatcgc cgtaggtatg gcaaccaaca tcccgccgca
      781 caacctgacg gaagtcatca acggttgtct ggcgtatatt gatgatgaag acatcagcat
      841 tgaagggctg atggaacaca tcccggggcc ggacttcccg acggcggcaa tcattaacgg
      901 tcgtcgcggt attgaagaag cttaccgtac cggtcgcggc aaggtgtata tccgcgctcg
      961 cgcagaagtg gaagttgacg ccaaaaccgg tcgtgaaacc attatcgtcc acgaaattcc
     1021 gtatcaggta aacaaagcgc gcctgatcga gaagattgcg gaactggtaa aagaaaaacg
     1081 cgtggaaggc atcagcgcgc tgcgtgacga gtctgacaaa gacggtatgc gcatcgtgat
     1141 tgaagtgaaa cgcgatgcgg tcggtgaagt tgtgctcaac aacctctact cccagaccca
     1201 gttgcaggtt tctttcggta tcaacatggt ggcattgcac catggtcagc cgaagatcat
     1261 gaacctgaaa gacatcatcg cggcgtttgt tcgtcaccgc cgtgaagtgg tgacccgtcg
     1321 tactattttc gaactgcgta aagctcgcga tcgtgctcat atccttgaag cattagccgt
     1381 ggcgctggcg aacatcgacc cgatcatcga actgatccgt catgcgccga cgccagcaga
     1441 agcgaaaact gcgctggttg ctaatccgtg gcagctgggc aacgttgccg cgatgctcga
     1501 acgtgctggc gacgatgctg cgcgtccgga atggctggag ccagagttcg gcgtgcgtga
     1561 tggtctgtac tacctgaccg aacagcaagc tcaggcgatt ctggatctgc gtttgcagaa
     1621 actgaccggt cttgagcacg aaaaactgct cgacgaatac aaagagctgc tggatcagat
     1681 cgcggaactg ttgcgtattc ttggtagcgc cgatcgtctg atggaagtga tccgtgaaga
     1741 gctggagctg gttcgtgaac agttcggtga caaacgtcgt actgaaatca ccgccaacag
     1801 cgcagacatc aacctggaag atctgatcac ccaggaagat gtggtcgtga cgctctctca
     1861 ccagggctac gttaagtatc agccgctttc tgaatacgaa gcgcagcgtc gtggcgggaa
     1921 aggtaaatct gccgcacgta ttaaagaaga agactttatc gaccgactgc tggtggcgaa
     1981 cactcacgac catattctgt gcttctccag ccgtggtcgc gtctattcga tgaaagttta
     2041 tcagttgccg gaagccactc gtggcgcgcg cggtcgtccg atcgtcaacc tgctgccgct
     2101 ggagcaggac gaacgtatca ctgcgatcct gccagtgacc gagtttgaag aaggcgtgaa
     2161 agtcttcatg gcgaccgcta acggtaccgt gaagaaaact gtcctcaccg agttcaaccg
     2221 tctgcgtacc gccggtaaag tggcgatcaa actggttgac ggcgatgagc tgatcggcgt
     2281 tgacctgacc agcggcgaag acgaagtaat gctgttctcc gctgaaggta aagtggtgcg
     2341 ctttaaagag tcttctgtcc gtgcgatggg ctgcaacacc accggtgttc gcggtattcg
     2401 cttaggtgaa ggcgataaag tcgtctctct gatcgtgcct cgtggcgatg gcgcaatcct
     2461 caccgcaacg caaaacggtt acggtaaacg taccgcagtg gcggaatacc caaccaagtc
     2521 gcgtgcgacg aaaggggtta tctccatcaa ggttaccgaa cgtaacggtt tagttgttgg
     2581 cgcggtacag gtagatgact gcgaccagat catgatgatc accgatgccg gtacgctggt
     2641 acgtactcgc gtttcggaaa tcagcatcgt gggccgtaac acccagggcg tgatcctcat
     2701 ccgtactgcg gaagatgaaa acgtagtggg tctgcaacgt gttgctgaac cggttgacga
     2761 ggaagatctg gataccatcg acggcagtgc cgcggaaggg gacgatgaaa tcgctccgga
     2821 agtggacgtt gacgacgagc cagaagaaga ataatactag agccaggcat caaataaaac
     2881 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2941 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//