LOCUS       BBa_K676010             2778 bp    DNA     linear       23-Dec-2024
DEFINITION  GyrB cassette
ACCESSION   BBa_K676010
VERSION     BBa_K676010.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..2641
                     /label=BBa_K676009
     stem_loop       2661..2704
                     /label=stem_loop
     misc_feature    2650..2729
                     /label=BBa_B0010
     terminator      2650..2729
                     /label=BBa_B0010
     stem_loop       2745..2764
                     /label=T7 TE
     misc_feature    2771..2771
                     /label=stop
     polyA_site      2765..2778
                     /label=polya
     misc_feature    2738..2778
                     /label=BBa_B0012
     terminator      2738..2778
                     /label=BBa_B0012
     terminator      2650..2778
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt cgaactctta
      241 tgactcctcc agtatcaaag tcctgaaagg gctggatgcg gtgcgtaagc gcccgggtat
      301 gtatatcggc gacacggatg acggcaccgg tctgcaccac atggtattcg aggtggtaga
      361 taacgctatc gacgaagcgc tcgcgggtca ctgtaaagaa attatcgtca ccattcacgc
      421 cgataactct gtctctgtac aggatgacgg gcgcggcatt ccgaccggta ttcacccgga
      481 agagggcgta tcggcggcgg aagtgatcat gaccgttctg cacgcaggcg gtaaatttga
      541 cgataactcc tataaagtgt ccggcggtct gcacggcgtt ggtgtttcgg tagtaaacgc
      601 cctgtcgcaa aaactggagc tggttatcca gcgcgagggt aaaattcacc gtcagatcta
      661 cgaacacggt gtaccgcagg ccccgctggc ggttaccggc gagactgaaa aaaccggcac
      721 catggtgcgt ttctggccca gcctcgaaac cttcaccaat gtgaccgagt tcgaatatga
      781 aattctggcg aaacgtctgc gtgagttgtc gttcctcaac tccggcgttt ccattcgtct
      841 gcgcgacaag cgcgacggca aagaagacca cttccactat gaaggcggca tcaaggcgtt
      901 cgttgaatat ctgaacaaga acaaaacgcc gatccacccg aatatcttct acttctccac
      961 tgaaaaagac ggtattggcg tcgaagtggc gttgcagtgg aacgatggct tccaggaaaa
     1021 catctactgc tttaccaaca acattccgca gcgtgacggc ggtactcacc tggcaggctt
     1081 ccgtgcggcg atgacccgta ccctgaacgc ctacatggac aaagaaggct acagcaaaaa
     1141 agccaaagtc agcgccaccg gtgacgatgc gcgtgaaggc ctgattgcgg tcgtttccgt
     1201 gaaagtgccg gacccgaaat tctcctccca gaccaaagac aaactggttt cttctgaggt
     1261 gaaatcggcg gttgaacagc agatgaacga actgctggca gaatacctgc tggaaaaccc
     1321 aaccgacgcg aaaatcgtgg ttggcaaaat tatcgatgct gcccgtgccc gtgaagcggc
     1381 gcgtcgcgcg cgtgaaatga cccgccgtaa aggtgcgctc gacttagcgg gcctgccggg
     1441 caaactggca gactgccagg aacgcgatcc ggcgctttcc gaactgtacc tggtggaagg
     1501 ggactccgcg ggcggctctg cgaagcaggg gcgtaaccgc aagaaccagg cgattctgcc
     1561 gctgaagggt aaaatcctca acgtcgagaa agcgcgcttc gataagatgc tctcttctca
     1621 ggaagtggcg acgcttatca ccgcgcttgg ctgtggtatc ggtcgtgacg agtacaaccc
     1681 ggacaaactg cgttatcaca gcatcatcat catgaccgat gcggacgtcg acggctcgca
     1741 cattcgtacg ctgctgttga ccttcttcta tcgtcagatg ccggaaatcg ttgaacgcgg
     1801 tcacgtctac atcgctcagc cgccgctgta caaagtgaag aaaggcaagc aggaacagta
     1861 cattaaagac gacgaagcga tggatcagta ccagatctct atcgcgctgg acggcgcaac
     1921 gctgcacacc aacgccagtg caccggcatt ggctggcgaa gcgttagaga aactggtatc
     1981 tgagtacaac gcgacgcaga aaatgatcaa tcgtatggag cgtcgttatc cgaaagcaat
     2041 gctgaaagag cttatctatc agccgacgtt gacggaagct gacctttctg atgagcagac
     2101 cgttacccgc tgggtgaacg cgctggtcag cgaactgaac gacaaagaac agcacggcag
     2161 ccagtggaag tttgatgttc acaccaatgc tgagcaaaac ctgttcgagc cgattgttcg
     2221 cgtgcgtacc cacggtgtgg atactgacta tccgctggat cacgagttta tcaccggtgg
     2281 cgaatatcgt cgtatctgca cgctgggtga gaaactgcgt ggcttgctgg aagaagatgc
     2341 gtttatcgaa cgtggcgagc gtcgtcagcc ggtagccagc ttcgagcagg cgctggactg
     2401 gctggtgaaa gagtcccgtc gcggcctctc catccagcgt tataaaggtc tgggcgagat
     2461 gaacccggaa cagctgtggg aaaccactat ggacccggaa agtcgtcgta tgctgcgcgt
     2521 taccgttaaa gatgcgattg ctgccgacca gttgttcacc acgctgatgg gcgacgccgt
     2581 tgaaccgcgc cgtgcgttta ttgaagagaa cgccctgaaa gcggcgaata tcgatattta
     2641 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2701 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2761 ggcctttctg cgtttata
//