LOCUS       BBa_K676013             1416 bp    DNA     linear       25-Nov-2024
DEFINITION  Mu/Spy Ligation
ACCESSION   BBa_K676013
VERSION     BBa_K676013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..363
                     /label=BBa_K676001
     misc_feature    36..321
                     /label=Mu Phage SGS
     misc_feature    1..363
                     /label=BBa_K676001
     promoter        521..528
                     /label=-10
     promoter        499..507
                     /label=-35
     misc_feature    375..393
                     /label=BaeR
     misc_feature    437..452
                     /label=BaeR
     misc_feature    478..491
                     /label=CpxR
     promoter        372..533
                     /label=BBa_K239001
     misc_feature    546..549
                     /label=conserved
     RBS             542..553
                     /label=BBa_B0034
     CDS             560..1279
                     /label=GFP protein
     CDS             560..1279
                     /label=BBa_E0040
     stem_loop       1299..1342
                     /label=stem_loop
     misc_feature    1288..1367
                     /label=BBa_B0010
     terminator      1288..1367
                     /label=BBa_B0010
     stem_loop       1383..1402
                     /label=T7 TE
     misc_feature    1409..1409
                     /label=stop
     polyA_site      1403..1416
                     /label=polya
     misc_feature    1376..1416
                     /label=BBa_B0012
     terminator      1376..1416
                     /label=BBa_B0012
     misc_feature    372..1416
                     /label=BBa_K239009
ORIGIN
        1 cggtacaccc gatatggcac cccgcccggc aggcgttcct gcgcgtcctt atatggggct
       61 ggataagacc ggggaacagg aaatttttga tgccatcaga aaacgcgtca gcgccgctct
      121 gaggcaataa acagaatcag gcataaaatc agccgcacag attttttaaa acgcgccacg
      181 ggatttttaa accggtattt aacggtgtat gaatcccgtt ttatcttcct ttcactttct
      241 ttctccagta ctcaaatagc ataaccccag attttcccgc acctcccgca aactgactgc
      301 tcacaaacca tgatgagcag caggacatga aaaaacacgc gattggcatt gccgcactga
      361 atgtactaga gagttcttca taatctctgc aaaatcatcg tgttgtaata ttctctcatc
      421 actctccatc aaattttctt tttttctcca taattggcgc aaaagtgttt tttacacttt
      481 cattgtttta ccgttgctct gattaattga cgctaaagtc agtaaagtta atctactaga
      541 gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
      601 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      661 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      721 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      781 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      841 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      901 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      961 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1021 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     1081 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     1141 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     1201 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     1261 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1321 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1381 ctggctcacc ttcgggtggg cctttctgcg tttata
//