BBa_K239001 1 BBa_K239001 Spy promoter, activated by Phosporylated CpxR, BaeR and Sigma70 2009-06-21T11:00:00Z 2015-05-08T01:11:36Z Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence. Sequence contains 2 phosphorylated BaeR binding sites, 1 phosphorylated CpxR binding site and transcription is initiated by sigma factor 70. Function: Can detect various periplasmic stresses. Area of application: Hopefully it can be used to detect presence of relevant unfolded proteins in the periplasmic space, to detect shear stress or other so far undefined relevant stress. false false _375_ 0 4247 9 It's complicated true Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first BaeR binding site and ends 5 pb after the -10 box. false Axel Nystrom annotation2006305 1 -10 range2006305 1 150 157 annotation2006309 1 CpxR range2006309 1 107 120 annotation2006306 1 -35 range2006306 1 128 136 annotation2006307 1 BaeR range2006307 1 4 22 annotation2006308 1 BaeR range2006308 1 66 81 BBa_K239009 1 BBa_K239009 GFP test devise for Spy promoter 2009-09-05T11:00:00Z 2015-05-08T01:11:36Z ... Hopefully the spy promoter can be used to detect presence of relevant unfolded proteins in the periplasmic space, to detect shear stress or other so far undefined relevant stress. false false _375_ 0 5136 9 It's complicated true ... false Xiang Chen component2018816 1 BBa_B0010 component2018811 1 BBa_K239001 component2018813 1 BBa_B0034 component2018815 1 BBa_E0040 component2018818 1 BBa_B0012 annotation2018818 1 BBa_B0012 range2018818 1 1005 1045 annotation2018816 1 BBa_B0010 range2018816 1 917 996 annotation2018811 1 BBa_K239001 range2018811 1 1 162 annotation2018813 1 BBa_B0034 range2018813 1 171 182 annotation2018815 1 BBa_E0040 range2018815 1 189 908 BBa_K676013 1 BBa_K676013 Mu/Spy Ligation 2011-09-18T11:00:00Z 2015-05-08T01:13:04Z 2009 stress device A device to detects the level of stress due to a GBS being present on the plasmid. false false _859_ 0 10148 9 Not in stock true Ligation false Meng Li component2134304 1 BBa_K676001 component2134321 1 BBa_K239009 annotation2134321 1 BBa_K239009 range2134321 1 372 1416 annotation2134304 1 BBa_K676001 range2134304 1 1 363 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K676001 1 BBa_K676001 Gyrase Binding Site from Mu Bacteriophage 2011-09-06T11:00:00Z 2015-05-08T01:13:03Z Mu bacteriophage genome The strong gyrase site clones from Mu bacteriophage. false false _859_ 0 9830 9 In stock true Cloned it from the bacteriophage genome by PCR using synthesised primers. false Meng Li annotation2149296 1 BBa_K676001 range2149296 1 1 363 annotation2149297 1 Mu Phage SGS range2149297 1 36 321 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K676013_sequence 1 cggtacacccgatatggcaccccgcccggcaggcgttcctgcgcgtccttatatggggctggataagaccggggaacaggaaatttttgatgccatcagaaaacgcgtcagcgccgctctgaggcaataaacagaatcaggcataaaatcagccgcacagattttttaaaacgcgccacgggatttttaaaccggtatttaacggtgtatgaatcccgttttatcttcctttcactttctttctccagtactcaaatagcataaccccagattttcccgcacctcccgcaaactgactgctcacaaaccatgatgagcagcaggacatgaaaaaacacgcgattggcattgccgcactgaatgtactagagagttcttcataatctctgcaaaatcatcgtgttgtaatattctctcatcactctccatcaaattttctttttttctccataattggcgcaaaagtgttttttacactttcattgttttaccgttgctctgattaattgacgctaaagtcagtaaagttaatctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K239009_sequence 1 agttcttcataatctctgcaaaatcatcgtgttgtaatattctctcatcactctccatcaaattttctttttttctccataattggcgcaaaagtgttttttacactttcattgttttaccgttgctctgattaattgacgctaaagtcagtaaagttaatctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K239001_sequence 1 agttcttcataatctctgcaaaatcatcgtgttgtaatattctctcatcactctccatcaaattttctttttttctccataattggcgcaaaagtgttttttacactttcattgttttaccgttgctctgattaattgacgctaaagtcagtaaagttaatc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K676001_sequence 1 cggtacacccgatatggcaccccgcccggcaggcgttcctgcgcgtccttatatggggctggataagaccggggaacaggaaatttttgatgccatcagaaaacgcgtcagcgccgctctgaggcaataaacagaatcaggcataaaatcagccgcacagattttttaaaacgcgccacgggatttttaaaccggtatttaacggtgtatgaatcccgttttatcttcctttcactttctttctccagtactcaaatagcataaccccagattttcccgcacctcccgcaaactgactgctcacaaaccatgatgagcagcaggacatgaaaaaacacgcgattggcattgccgcactgaatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z