BBa_K676014
type: ComponentDefinition
persistent identity: BBa K676014
display id: BBa_K676014
version: 1
was generated by: iGEM to SBOL conversion
description: Extractery
created: 2011-09-23T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: Designed
owned by: james
owned by: myers
mutable description: Designed
discontinued: true
dominant: false
experience: experience/None
group u list: _859_
m user id: 0
owner id: 10148
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: Designed
star: false
creator: Ejaj Intisar
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I723020
component: BBa_B0015
component: BBa_K112806
component: BBa_B0034
component: BBa_R0051
component: BBa_I723017
component: BBa_K098997
component: BBa_J23114
component: BBa_K112805
component: BBa_K676006
sequence annotation: BBa_J23114
sequence annotation: BBa_B0034
sequence annotation: BBa_K098997
sequence annotation: BBa_I723017
sequence annotation: BBa_B0015
sequence annotation: BBa_R0051
sequence annotation: BBa_K112806
sequence annotation: BBa_I723020
sequence annotation: BBa_K676006
sequence annotation: BBa_K112805
sequence: https://synbiohub.org/public/igem/BBa_K676014_sequence/1

BBa_I723020
type: Component
persistent identity: component2139007
display id: component2139007
version: 1
top level: BBa_K676014
definition: Pu
access: public

BBa_B0015
type: Component
persistent identity: component2139005
display id: component2139005
version: 1
top level: BBa_K676014
definition: BBa_B0015
access: public

BBa_K112806
type: Component
persistent identity: component2138998
display id: component2138998
version: 1
top level: BBa_K676014
definition: BBa_K112806
access: public

BBa_B0034
type: Component
persistent identity: component2138996
display id: component2138996
version: 1
top level: BBa_K676014
definition: BBa_B0034
access: public

BBa_R0051
type: Component
persistent identity: component2138991
display id: component2138991
version: 1
top level: BBa_K676014
definition: cI lam
access: public

BBa_B0015
type: Component
persistent identity: component2138989
display id: component2138989
version: 1
top level: BBa_K676014
definition: BBa_B0015
access: public

BBa_I723017
type: Component
persistent identity: component2138982
display id: component2138982
version: 1
top level: BBa_K676014
definition: XylR
access: public

BBa_K098997
type: Component
persistent identity: component2138979
display id: component2138979
version: 1
top level: BBa_K676014
definition: BBa_K098997
access: public

BBa_B0034
type: Component
persistent identity: component2138977
display id: component2138977
version: 1
top level: BBa_K676014
definition: BBa_B0034
access: public

BBa_J23114
type: Component
persistent identity: component2138975
display id: component2138975
version: 1
top level: BBa_K676014
definition: BBa_J23114
access: public

BBa_B0015
type: Component
persistent identity: component2139017
display id: component2139017
version: 1
top level: BBa_K676014
definition: BBa_B0015
access: public

BBa_K112805
type: Component
persistent identity: component2139010
display id: component2139010
version: 1
top level: BBa_K676014
definition: BBa_K112805
access: public

BBa_K676006
type: Component
persistent identity: component2139008
display id: component2139008
version: 1
top level: BBa_K676014
definition: BBa_K676006
access: public

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2138975
display id: annotation2138975
version: 1
top level: BBa_K676014
location: https://synbiohub.org/public/igem/BBa_K676014/annotation2138975/range2138975/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K676014/annotation2138975/range2138975/1
type: Range
persistent identity: range2138975
display id: range2138975
version: 1
top level: BBa_K676014
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2138977
display id: annotation2138977
version: 1
top level: BBa_K676014
location: https://synbiohub.org/public/igem/BBa_K676014/annotation2138977/range2138977/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K676014/annotation2138977/range2138977/1
type: Range
persistent identity: range2138977
display id: range2138977
version: 1
top level: BBa_K676014
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K098997
type: SequenceAnnotation
persistent identity: annotation2138979
display id: annotation2138979
version: 1
top level: BBa_K676014
location: https://synbiohub.org/public/igem/BBa_K676014/annotation2138979/range2138979/1
component: BBa_K098997

https://synbiohub.org/public/igem/BBa_K676014/annotation2138979/range2138979/1
type: Range
persistent identity: range2138979
display id: range2138979
version: 1
top level: BBa_K676014
direction: direction/reverse
start: 62
end: 775
orientation: inline

BBa_I723017
type: SequenceAnnotation
persistent identity: annotation2138982
display id: annotation2138982
version: 1
top level: BBa_K676014
location: https://synbiohub.org/public/igem/BBa_K676014/annotation2138982/range2138982/1
component: BBa_I723017

https://synbiohub.org/public/igem/BBa_K676014/annotation2138982/range2138982/1
type: Range
persistent identity: range2138982
display id: range2138982
version: 1
top level: BBa_K676014
direction: direction/reverse
start: 782
end: 2482
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2138989
display id: annotation2138989
version: 1
top level: BBa_K676014
location: https://synbiohub.org/public/igem/BBa_K676014/annotation2138989/range2138989/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K676014/annotation2138989/range2138989/1
type: Range
persistent identity: range2138989
display id: range2138989
version: 1
top level: BBa_K676014
direction: direction/reverse
start: 2491
end: 2619
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2138991
display id: annotation2138991
version: 1
top level: BBa_K676014
location: https://synbiohub.org/public/igem/BBa_K676014/annotation2138991/range2138991/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K676014/annotation2138991/range2138991/1
type: Range
persistent identity: range2138991
display id: range2138991
version: 1
top level: BBa_K676014
direction: direction/forward
start: 2628
end: 2676
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2138996
display id: annotation2138996
version: 1
top level: BBa_K676014
location: https://synbiohub.org/public/igem/BBa_K676014/annotation2138996/range2138996/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K676014/annotation2138996/range2138996/1
type: Range
persistent identity: range2138996
display id: range2138996
version: 1
top level: BBa_K676014
direction: direction/reverse
start: 2685
end: 2696
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2138998
display id: annotation2138998
version: 1
top level: BBa_K676014
location: https://synbiohub.org/public/igem/BBa_K676014/annotation2138998/range2138998/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K676014/annotation2138998/range2138998/1
type: Range
persistent identity: range2138998
display id: range2138998
version: 1
top level: BBa_K676014
direction: direction/reverse
start: 2705
end: 3218
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2139005
display id: annotation2139005
version: 1
top level: BBa_K676014
location: https://synbiohub.org/public/igem/BBa_K676014/annotation2139005/range2139005/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K676014/annotation2139005/range2139005/1
type: Range
persistent identity: range2139005
display id: range2139005
version: 1
top level: BBa_K676014
direction: direction/reverse
start: 3227
end: 3355
orientation: inline

BBa_I723020
type: SequenceAnnotation
persistent identity: annotation2139007
display id: annotation2139007
version: 1
top level: BBa_K676014
location: https://synbiohub.org/public/igem/BBa_K676014/annotation2139007/range2139007/1
component: BBa_I723020

https://synbiohub.org/public/igem/BBa_K676014/annotation2139007/range2139007/1
type: Range
persistent identity: range2139007
display id: range2139007
version: 1
top level: BBa_K676014
direction: direction/reverse
start: 3364
end: 3683
orientation: inline

BBa_K676006
type: SequenceAnnotation
persistent identity: annotation2139008
display id: annotation2139008
version: 1
top level: BBa_K676014
location: https://synbiohub.org/public/igem/BBa_K676014/annotation2139008/range2139008/1
component: BBa_K676006

https://synbiohub.org/public/igem/BBa_K676014/annotation2139008/range2139008/1
type: Range
persistent identity: range2139008
display id: range2139008
version: 1
top level: BBa_K676014
direction: direction/reverse
start: 3692
end: 4583
orientation: inline

BBa_K112805
type: SequenceAnnotation
persistent identity: annotation2139010
display id: annotation2139010
version: 1
top level: BBa_K676014
location: https://synbiohub.org/public/igem/BBa_K676014/annotation2139010/range2139010/1
component: BBa_K112805

https://synbiohub.org/public/igem/BBa_K676014/annotation2139010/range2139010/1
type: Range
persistent identity: range2139010
display id: range2139010
version: 1
top level: BBa_K676014
direction: direction/reverse
start: 4592
end: 5267
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2139017
display id: annotation2139017
version: 1
top level: BBa_K676014
location: https://synbiohub.org/public/igem/BBa_K676014/annotation2139017/range2139017/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K676014/annotation2139017/range2139017/1
type: Range
persistent identity: range2139017
display id: range2139017
version: 1
top level: BBa_K676014
direction: direction/reverse
start: 5276
end: 5404
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

XylR
type: ComponentDefinition
persistent identity: BBa I723017
display id: BBa_I723017
version: 1
was generated by: iGEM to SBOL conversion
description: XylR coding region
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
mutable description: atgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctgcagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgcgcgagcgcgtggaagatatcccgctgctggtcgagcattttcttagaaggcaccataaggaatacggtaagaagactcttggcctgtctgatcgagcgatggaggcctgcctccactaccaatggccaggcaatatccgcgagctggagaacgcccttgagcgcggggtgattcttaccgagagcaacgaaagcatcaatgtcgagtcgctgttcccggggttggcgacggctaccgaaggcgacaggctatcgagcgagggccggttggaggaggagtccggtgacagttggtttaggcaaattatcgaccagggcgtcagcctcgaagatctcgaagcgggtttaatgcgcacggccatggaccgttgtgggcagaatatctcacaggcggcgcggttgctgggattgacccgcccggcaatggcctatcgacttaagaagcttgaccccagcttatctgtgaaagcaatgggccgatag
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: [Christine/Maia]
star: false
creator: Scott Ramsay
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: XylR
sequence annotation: PstI site
sequence: https://synbiohub.org/public/igem/BBa_I723017_sequence/1

XylR
type: SequenceAnnotation
persistent identity: annotation1954931
display id: annotation1954931
version: 1
top level: XylR
location: https://synbiohub.org/public/igem/BBa_I723017/annotation1954931/range1954931/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I723017/annotation1954931/range1954931/1
type: Range
persistent identity: range1954931
display id: range1954931
version: 1
top level: XylR
direction: direction/forward
start: 1
end: 1701
orientation: inline

PstI site
type: SequenceAnnotation
persistent identity: annotation1954930
display id: annotation1954930
version: 1
top level: XylR
location: https://synbiohub.org/public/igem/BBa_I723017/annotation1954930/range1954930/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I723017/annotation1954930/range1954930/1
type: Range
persistent identity: range1954930
display id: range1954930
version: 1
top level: XylR
direction: direction/forward
start: 643
end: 648
orientation: inline

BBa_J23114
type: ComponentDefinition
persistent identity: BBa J23114
display id: BBa_J23114
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23114_sequence/1

BBa_K098997
type: ComponentDefinition
persistent identity: BBa K098997
display id: BBa_K098997
version: 1
was generated by: iGEM to SBOL conversion
description: cIts coding region
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:41Z
mutable provenance: It comes from PGW7 plasmid.
owned by: james
owned by: myers
mutable description: This it the temperature sensitive cI from the PGW7 plasmid. It has the Elowitz RBS in front (BBa_B0034). It produces a temperature sensitive cI repressor that loses binding efficiency with the cI promoter between 35 and 42 degrees C.
discontinued: false
dominant: false
experience: experience/None
group u list: _196_
m user id: 0
owner id: 3394
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Obtained by PCR. RBS also added by PCR.
star: false
creator: Harvard iGEM 08
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cIts
sequence: https://synbiohub.org/public/igem/BBa_K098997_sequence/1

cIts
type: SequenceAnnotation
persistent identity: annotation1988780
display id: annotation1988780
version: 1
top level: BBa_K098997
location: https://synbiohub.org/public/igem/BBa_K098997/annotation1988780/range1988780/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K098997/annotation1988780/range1988780/1
type: Range
persistent identity: range1988780
display id: range1988780
version: 1
top level: BBa_K098997
direction: direction/forward
start: 1
end: 714
orientation: inline

Pu
type: ComponentDefinition
persistent identity: BBa I723020
display id: BBa_I723020
version: 1
was generated by: iGEM to SBOL conversion
description: Pu
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
mutable description: This is the promoter activated by the transcriptional regulator XylR (BBa_I723136) in response to detection of environmental xylene.
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: [Christine/Maia]
star: true
creator: Scott Ramsay
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Pu
sequence: https://synbiohub.org/public/igem/BBa_I723020_sequence/1

Pu
type: SequenceAnnotation
persistent identity: annotation1955192
display id: annotation1955192
version: 1
top level: Pu
location: https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1
type: Range
persistent identity: range1955192
display id: range1955192
version: 1
top level: Pu
direction: direction/forward
start: 1
end: 320
orientation: inline

BBa_K676006
type: ComponentDefinition
persistent identity: BBa K676006
display id: BBa_K676006
version: 1
was generated by: iGEM to SBOL conversion
description: T5 Phage Exonuclease
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:13:03Z
mutable provenance: T5 Bacteriophage
owned by: james
owned by: myers
mutable description: exonuclease
discontinued: false
dominant: false
group u list: _859_
m user id: 0
owner id: 10148
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: sdm
star: false
creator: Ejaj Intisar
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: T5 Phage Exonuclease
sequence annotation: start
sequence annotation: BBa_K676006
sequence annotation: RL
sequence annotation: 1
sequence: https://synbiohub.org/public/igem/BBa_K676006_sequence/1

T5 Phage Exonuclease
type: SequenceAnnotation
persistent identity: annotation2148687
display id: annotation2148687
version: 1
top level: BBa_K676006
location: https://synbiohub.org/public/igem/BBa_K676006/annotation2148687/range2148687/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K676006/annotation2148687/range2148687/1
type: Range
persistent identity: range2148687
display id: range2148687
version: 1
top level: BBa_K676006
direction: direction/forward
start: 1
end: 876
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148685
display id: annotation2148685
version: 1
top level: BBa_K676006
location: https://synbiohub.org/public/igem/BBa_K676006/annotation2148685/range2148685/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K676006/annotation2148685/range2148685/1
type: Range
persistent identity: range2148685
display id: range2148685
version: 1
top level: BBa_K676006
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_K676006
type: SequenceAnnotation
persistent identity: annotation2148692
display id: annotation2148692
version: 1
top level: BBa_K676006
location: https://synbiohub.org/public/igem/BBa_K676006/annotation2148692/range2148692/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K676006/annotation2148692/range2148692/1
type: Range
persistent identity: range2148692
display id: range2148692
version: 1
top level: BBa_K676006
direction: direction/forward
start: 1
end: 876
orientation: inline

RL
type: SequenceAnnotation
persistent identity: annotation2148689
display id: annotation2148689
version: 1
top level: BBa_K676006
location: https://synbiohub.org/public/igem/BBa_K676006/annotation2148689/range2148689/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K676006/annotation2148689/range2148689/1
type: Range
persistent identity: range2148689
display id: range2148689
version: 1
top level: BBa_K676006
direction: direction/forward
start: 226
end: 231
orientation: inline

1
type: SequenceAnnotation
persistent identity: annotation2148686
display id: annotation2148686
version: 1
top level: BBa_K676006
location: https://synbiohub.org/public/igem/BBa_K676006/annotation2148686/range2148686/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K676006/annotation2148686/range2148686/1
type: Range
persistent identity: range2148686
display id: range2148686
version: 1
top level: BBa_K676006
direction: direction/forward
start: 874
end: 876
orientation: inline

BBa_K112805
type: ComponentDefinition
persistent identity: BBa K112805
display id: BBa_K112805
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 holin]
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=160221&to=160877&view=gbwithparts
owned by: james
owned by: myers
mutable description: Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer.
discontinued: false
dominant: true
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Making composite part of rbs.holin is problematic unless antiholin is expressed in bacteria together. Antiholin binds holin and inhibits the formation of holin.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 holin
sequence: https://synbiohub.org/public/igem/BBa_K112805_sequence/1

T4 holin
type: SequenceAnnotation
persistent identity: annotation1999005
display id: annotation1999005
version: 1
top level: BBa_K112805
location: https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1
type: Range
persistent identity: range1999005
display id: range1999005
version: 1
top level: BBa_K112805
direction: direction/forward
start: 17
end: 673
orientation: inline

BBa_K112806
type: ComponentDefinition
persistent identity: BBa K112806
display id: BBa_K112806
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 endolysin]
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer.
discontinued: false
dominant: true
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: In stock
status: status/Available
bookmark: false
mutable notes: High expression of lysozyme only is toxic to bacteria.
star: false
creator: Jin Huh
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: T4 Endolysin
sequence: https://synbiohub.org/public/igem/BBa_K112806_sequence/1

T4 Endolysin
type: SequenceAnnotation
persistent identity: annotation1999018
display id: annotation1999018
version: 1
top level: BBa_K112806
location: https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
type: Range
persistent identity: range1999018
display id: range1999018
version: 1
top level: BBa_K112806
direction: direction/forward
start: 17
end: 511
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K676014_sequence/1
type: Sequence
persistent identity: BBa K676014 sequence
display id: BBa_K676014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgatactagatgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctgcagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I723017_sequence/1
type: Sequence
persistent identity: BBa I723017 sequence
display id: BBa_I723017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctgcagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgcgcgagcgcgtggaagatatcccgctgctggtcgagcattttcttagaaggcaccataaggaatacggtaagaagactcttggcctgtctgatcgagcgatggaggcctgcctccactaccaatggccaggcaatatccgcgagctggagaacgcccttgagcgcggggtgattcttaccgagagcaacgaaagcatcaatgtcgagtcgctgttcccggggttggcgacggctaccgaaggcgacaggctatcgagcgagggccggttggaggaggagtccggtgacagttggtttaggcaaattatcgaccagggcgtcagcctcgaagatctcgaagcgggtttaatgcgcacggccatggaccgttgtgggcagaatatctcacaggcggcgcggttgctgggattgacccgcccggcaatggcctatcgacttaagaagcttgaccccagcttatctgtgaaagcaatgggccgatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I723020_sequence/1
type: Sequence
persistent identity: BBa I723020 sequence
display id: BBa_I723020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23114_sequence/1
type: Sequence
persistent identity: BBa J23114 sequence
display id: BBa_J23114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K098997_sequence/1
type: Sequence
persistent identity: BBa K098997 sequence
display id: BBa_K098997_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K676006_sequence/1
type: Sequence
persistent identity: BBa K676006 sequence
display id: BBa_K676006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtaaatcctggggaaaatttattgaagaagaggaagctgaaatggcttcccgtcgtaatctaatgattgtcgatggaactaacttaggctttcgcttcaaacataacaatagtaaaaaaccatttgcctcaagttatgtttcaactattcaatctctggcaaaatcctactctgccagaactacgattgttctaggtgataagggaaaatccgtatttcgcttggaacatctaccagagtataaaggtaatcgtgatgaaaagtacgcacaacgtacggaagaggagaaagcgctagatgagcagttctttgagtatttgaaggatgctttcgagttgtgtaaaactacattcccaacttttaccattcgtggtgtagaagcagacgatatggcagcttatattgttaagctcatcgggcatctttatgatcacgtttggctaatatctacagatggtgactgggatactttattaacggataaagtttctcgtttttctttcacaacacgtcgtgagtatcatcttcgtgatatgtatgaacatcataatgttgatgatgttgagcagtttatctccctgaaagcaattatgggagatctaggagataatattcgtggtgttgaaggaataggagcaaaacgcggatataatattattcgtgagtttggtaacgtactggatattattgatcagcttccactgcctggaaagcagaaatatatacagaacctgaatgcatcggaagaactgcttttccgaaacttgattctggttgatttacctacctactgtgtggatgctattgctgctgtaggtcaagatgtgttagataagtttacaaaagatattttggagattgcagaacaatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112805_sequence/1
type: Sequence
persistent identity: BBa K112805 sequence
display id: BBa_K112805_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112806_sequence/1
type: Sequence
persistent identity: BBa K112806 sequence
display id: BBa_K112806_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z