LOCUS BBa_K676016 5412 bp DNA linear 21-Dec-2024 DEFINITION Gyrase cassette ACCESSION BBa_K676016 VERSION BBa_K676016.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 227..229 /label=start misc_feature 2852..2854 /label=1 CDS 227..2854 /label=Gyrase A misc_feature 227..2854 /label=BBa_K676007 misc_feature 227..2854 /label=BBa_K676007 misc_feature 2861..5275 /label=BBa_K676009 stem_loop 5295..5338 /label=stem_loop misc_feature 5284..5363 /label=BBa_B0010 terminator 5284..5363 /label=BBa_B0010 stem_loop 5379..5398 /label=T7 TE misc_feature 5405..5405 /label=stop polyA_site 5399..5412 /label=polya misc_feature 5372..5412 /label=BBa_B0012 terminator 5372..5412 /label=BBa_B0012 terminator 5284..5412 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcgaccttgc 241 gagagaaatt acaccggtca acattgagga agagctgaag agctcctatc tggattatgc 301 gatgtcggtc attgttggcc gtgcgctgcc agatgtccga gatggcctga agccggtaca 361 ccgtcgcgta ctttacgcca tgaacgtact aggcaatgac tggaacaaag cctataaaaa 421 atctgcccgt gtcgttggtg acgtaatcgg taaataccat ccccatggtg actcggcggt 481 ctatgacacg atcgtccgca tggcgcagcc attctcgctg cgttatatgc tggtagacgg 541 tcagggtaac ttcggttcta tcgacggcga ctctgcggcg gcaatgcgtt atacggaaat 601 ccgtctggcg aaaattgccc atgaactgat ggccgatctc gaaaaagaga cggtcgattt 661 cgttgataac tatgacggca cggaaaaaat tccggacgtc atgccaacca aaattcctaa 721 cctgctggtg aacggttctt ccggtatcgc cgtaggtatg gcaaccaaca tcccgccgca 781 caacctgacg gaagtcatca acggttgtct ggcgtatatt gatgatgaag acatcagcat 841 tgaagggctg atggaacaca tcccggggcc ggacttcccg acggcggcaa tcattaacgg 901 tcgtcgcggt attgaagaag cttaccgtac cggtcgcggc aaggtgtata tccgcgctcg 961 cgcagaagtg gaagttgacg ccaaaaccgg tcgtgaaacc attatcgtcc acgaaattcc 1021 gtatcaggta aacaaagcgc gcctgatcga gaagattgcg gaactggtaa aagaaaaacg 1081 cgtggaaggc atcagcgcgc tgcgtgacga gtctgacaaa gacggtatgc gcatcgtgat 1141 tgaagtgaaa cgcgatgcgg tcggtgaagt tgtgctcaac aacctctact cccagaccca 1201 gttgcaggtt tctttcggta tcaacatggt ggcattgcac catggtcagc cgaagatcat 1261 gaacctgaaa gacatcatcg cggcgtttgt tcgtcaccgc cgtgaagtgg tgacccgtcg 1321 tactattttc gaactgcgta aagctcgcga tcgtgctcat atccttgaag cattagccgt 1381 ggcgctggcg aacatcgacc cgatcatcga actgatccgt catgcgccga cgccagcaga 1441 agcgaaaact gcgctggttg ctaatccgtg gcagctgggc aacgttgccg cgatgctcga 1501 acgtgctggc gacgatgctg cgcgtccgga atggctggag ccagagttcg gcgtgcgtga 1561 tggtctgtac tacctgaccg aacagcaagc tcaggcgatt ctggatctgc gtttgcagaa 1621 actgaccggt cttgagcacg aaaaactgct cgacgaatac aaagagctgc tggatcagat 1681 cgcggaactg ttgcgtattc ttggtagcgc cgatcgtctg atggaagtga tccgtgaaga 1741 gctggagctg gttcgtgaac agttcggtga caaacgtcgt actgaaatca ccgccaacag 1801 cgcagacatc aacctggaag atctgatcac ccaggaagat gtggtcgtga cgctctctca 1861 ccagggctac gttaagtatc agccgctttc tgaatacgaa gcgcagcgtc gtggcgggaa 1921 aggtaaatct gccgcacgta ttaaagaaga agactttatc gaccgactgc tggtggcgaa 1981 cactcacgac catattctgt gcttctccag ccgtggtcgc gtctattcga tgaaagttta 2041 tcagttgccg gaagccactc gtggcgcgcg cggtcgtccg atcgtcaacc tgctgccgct 2101 ggagcaggac gaacgtatca ctgcgatcct gccagtgacc gagtttgaag aaggcgtgaa 2161 agtcttcatg gcgaccgcta acggtaccgt gaagaaaact gtcctcaccg agttcaaccg 2221 tctgcgtacc gccggtaaag tggcgatcaa actggttgac ggcgatgagc tgatcggcgt 2281 tgacctgacc agcggcgaag acgaagtaat gctgttctcc gctgaaggta aagtggtgcg 2341 ctttaaagag tcttctgtcc gtgcgatggg ctgcaacacc accggtgttc gcggtattcg 2401 cttaggtgaa ggcgataaag tcgtctctct gatcgtgcct cgtggcgatg gcgcaatcct 2461 caccgcaacg caaaacggtt acggtaaacg taccgcagtg gcggaatacc caaccaagtc 2521 gcgtgcgacg aaaggggtta tctccatcaa ggttaccgaa cgtaacggtt tagttgttgg 2581 cgcggtacag gtagatgact gcgaccagat catgatgatc accgatgccg gtacgctggt 2641 acgtactcgc gtttcggaaa tcagcatcgt gggccgtaac acccagggcg tgatcctcat 2701 ccgtactgcg gaagatgaaa acgtagtggg tctgcaacgt gttgctgaac cggttgacga 2761 ggaagatctg gataccatcg acggcagtgc cgcggaaggg gacgatgaaa tcgctccgga 2821 agtggacgtt gacgacgagc cagaagaaga ataatactag atgtcgaact cttatgactc 2881 ctccagtatc aaagtcctga aagggctgga tgcggtgcgt aagcgcccgg gtatgtatat 2941 cggcgacacg gatgacggca ccggtctgca ccacatggta ttcgaggtgg tagataacgc 3001 tatcgacgaa gcgctcgcgg gtcactgtaa agaaattatc gtcaccattc acgccgataa 3061 ctctgtctct gtacaggatg acgggcgcgg cattccgacc ggtattcacc cggaagaggg 3121 cgtatcggcg gcggaagtga tcatgaccgt tctgcacgca ggcggtaaat ttgacgataa 3181 ctcctataaa gtgtccggcg gtctgcacgg cgttggtgtt tcggtagtaa acgccctgtc 3241 gcaaaaactg gagctggtta tccagcgcga gggtaaaatt caccgtcaga tctacgaaca 3301 cggtgtaccg caggccccgc tggcggttac cggcgagact gaaaaaaccg gcaccatggt 3361 gcgtttctgg cccagcctcg aaaccttcac caatgtgacc gagttcgaat atgaaattct 3421 ggcgaaacgt ctgcgtgagt tgtcgttcct caactccggc gtttccattc gtctgcgcga 3481 caagcgcgac ggcaaagaag accacttcca ctatgaaggc ggcatcaagg cgttcgttga 3541 atatctgaac aagaacaaaa cgccgatcca cccgaatatc ttctacttct ccactgaaaa 3601 agacggtatt ggcgtcgaag tggcgttgca gtggaacgat ggcttccagg aaaacatcta 3661 ctgctttacc aacaacattc cgcagcgtga cggcggtact cacctggcag gcttccgtgc 3721 ggcgatgacc cgtaccctga acgcctacat ggacaaagaa ggctacagca aaaaagccaa 3781 agtcagcgcc accggtgacg atgcgcgtga aggcctgatt gcggtcgttt ccgtgaaagt 3841 gccggacccg aaattctcct cccagaccaa agacaaactg gtttcttctg aggtgaaatc 3901 ggcggttgaa cagcagatga acgaactgct ggcagaatac ctgctggaaa acccaaccga 3961 cgcgaaaatc gtggttggca aaattatcga tgctgcccgt gcccgtgaag cggcgcgtcg 4021 cgcgcgtgaa atgacccgcc gtaaaggtgc gctcgactta gcgggcctgc cgggcaaact 4081 ggcagactgc caggaacgcg atccggcgct ttccgaactg tacctggtgg aaggggactc 4141 cgcgggcggc tctgcgaagc aggggcgtaa ccgcaagaac caggcgattc tgccgctgaa 4201 gggtaaaatc ctcaacgtcg agaaagcgcg cttcgataag atgctctctt ctcaggaagt 4261 ggcgacgctt atcaccgcgc ttggctgtgg tatcggtcgt gacgagtaca acccggacaa 4321 actgcgttat cacagcatca tcatcatgac cgatgcggac gtcgacggct cgcacattcg 4381 tacgctgctg ttgaccttct tctatcgtca gatgccggaa atcgttgaac gcggtcacgt 4441 ctacatcgct cagccgccgc tgtacaaagt gaagaaaggc aagcaggaac agtacattaa 4501 agacgacgaa gcgatggatc agtaccagat ctctatcgcg ctggacggcg caacgctgca 4561 caccaacgcc agtgcaccgg cattggctgg cgaagcgtta gagaaactgg tatctgagta 4621 caacgcgacg cagaaaatga tcaatcgtat ggagcgtcgt tatccgaaag caatgctgaa 4681 agagcttatc tatcagccga cgttgacgga agctgacctt tctgatgagc agaccgttac 4741 ccgctgggtg aacgcgctgg tcagcgaact gaacgacaaa gaacagcacg gcagccagtg 4801 gaagtttgat gttcacacca atgctgagca aaacctgttc gagccgattg ttcgcgtgcg 4861 tacccacggt gtggatactg actatccgct ggatcacgag tttatcaccg gtggcgaata 4921 tcgtcgtatc tgcacgctgg gtgagaaact gcgtggcttg ctggaagaag atgcgtttat 4981 cgaacgtggc gagcgtcgtc agccggtagc cagcttcgag caggcgctgg actggctggt 5041 gaaagagtcc cgtcgcggcc tctccatcca gcgttataaa ggtctgggcg agatgaaccc 5101 ggaacagctg tgggaaacca ctatggaccc ggaaagtcgt cgtatgctgc gcgttaccgt 5161 taaagatgcg attgctgccg accagttgtt caccacgctg atgggcgacg ccgttgaacc 5221 gcgccgtgcg tttattgaag agaacgccct gaaagcggcg aatatcgata tttaatacta 5281 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 5341 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 5401 tctgcgttta ta //