##gff-version 3
##sequence-region BBa_K676016 1 5412
BBa_K676016	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K676016	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K676016	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K676016	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K676016	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K676016	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K676016	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K676016	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K676016	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K676016	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K676016	.	terminator	5284	5412	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K676016	.	terminator	5284	5363	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K676016	.	stem_loop	5295	5338	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K676016	.	engineered_region	5284	5363	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K676016	.	terminator	5372	5412	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K676016	.	engineered_region	5372	5412	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K676016	.	stop_codon	5405	5405	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K676016	.	polyA_site	5399	5412	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K676016	.	stem_loop	5379	5398	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K676016	.	sequence_feature	2861	5275	.	+	0	ID=BBa_K676009;Name=BBa_K676009
BBa_K676016	.	sequence_feature	227	2854	.	+	0	ID=BBa_K676007;Name=BBa_K676007
BBa_K676016	.	engineered_region	227	2854	.	+	0	ID=annotation2148697;Name=BBa_K676007;Parent=BBa_K676007
BBa_K676016	.	CDS	227	2854	.	+	0	ID=annotation2148696;Name=Gyrase A;Parent=BBa_K676007
BBa_K676016	.	start_codon	227	229	.	+	0	ID=annotation2148694;Name=start;Parent=BBa_K676007
BBa_K676016	.	stop_codon	2852	2854	.	+	0	ID=annotation2148695;Name=1;Parent=BBa_K676007
>BBa_K676016
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgagcgacct
tgcgagagaaattacaccggtcaacattgaggaagagctgaagagctcctatctggattatgcgatgtcggtcattgtt
ggccgtgcgctgccagatgtccgagatggcctgaagccggtacaccgtcgcgtactttacgccatgaacgtactaggca
atgactggaacaaagcctataaaaaatctgcccgtgtcgttggtgacgtaatcggtaaataccatccccatggtgactc
ggcggtctatgacacgatcgtccgcatggcgcagccattctcgctgcgttatatgctggtagacggtcagggtaacttc
ggttctatcgacggcgactctgcggcggcaatgcgttatacggaaatccgtctggcgaaaattgcccatgaactgatgg
ccgatctcgaaaaagagacggtcgatttcgttgataactatgacggcacggaaaaaattccggacgtcatgccaaccaa
aattcctaacctgctggtgaacggttcttccggtatcgccgtaggtatggcaaccaacatcccgccgcacaacctgacg
gaagtcatcaacggttgtctggcgtatattgatgatgaagacatcagcattgaagggctgatggaacacatcccggggc
cggacttcccgacggcggcaatcattaacggtcgtcgcggtattgaagaagcttaccgtaccggtcgcggcaaggtgta
tatccgcgctcgcgcagaagtggaagttgacgccaaaaccggtcgtgaaaccattatcgtccacgaaattccgtatcag
gtaaacaaagcgcgcctgatcgagaagattgcggaactggtaaaagaaaaacgcgtggaaggcatcagcgcgctgcgtg
acgagtctgacaaagacggtatgcgcatcgtgattgaagtgaaacgcgatgcggtcggtgaagttgtgctcaacaacct
ctactcccagacccagttgcaggtttctttcggtatcaacatggtggcattgcaccatggtcagccgaagatcatgaac
ctgaaagacatcatcgcggcgtttgttcgtcaccgccgtgaagtggtgacccgtcgtactattttcgaactgcgtaaag
ctcgcgatcgtgctcatatccttgaagcattagccgtggcgctggcgaacatcgacccgatcatcgaactgatccgtca
tgcgccgacgccagcagaagcgaaaactgcgctggttgctaatccgtggcagctgggcaacgttgccgcgatgctcgaa
cgtgctggcgacgatgctgcgcgtccggaatggctggagccagagttcggcgtgcgtgatggtctgtactacctgaccg
aacagcaagctcaggcgattctggatctgcgtttgcagaaactgaccggtcttgagcacgaaaaactgctcgacgaata
caaagagctgctggatcagatcgcggaactgttgcgtattcttggtagcgccgatcgtctgatggaagtgatccgtgaa
gagctggagctggttcgtgaacagttcggtgacaaacgtcgtactgaaatcaccgccaacagcgcagacatcaacctgg
aagatctgatcacccaggaagatgtggtcgtgacgctctctcaccagggctacgttaagtatcagccgctttctgaata
cgaagcgcagcgtcgtggcgggaaaggtaaatctgccgcacgtattaaagaagaagactttatcgaccgactgctggtg
gcgaacactcacgaccatattctgtgcttctccagccgtggtcgcgtctattcgatgaaagtttatcagttgccggaag
ccactcgtggcgcgcgcggtcgtccgatcgtcaacctgctgccgctggagcaggacgaacgtatcactgcgatcctgcc
agtgaccgagtttgaagaaggcgtgaaagtcttcatggcgaccgctaacggtaccgtgaagaaaactgtcctcaccgag
ttcaaccgtctgcgtaccgccggtaaagtggcgatcaaactggttgacggcgatgagctgatcggcgttgacctgacca
gcggcgaagacgaagtaatgctgttctccgctgaaggtaaagtggtgcgctttaaagagtcttctgtccgtgcgatggg
ctgcaacaccaccggtgttcgcggtattcgcttaggtgaaggcgataaagtcgtctctctgatcgtgcctcgtggcgat
ggcgcaatcctcaccgcaacgcaaaacggttacggtaaacgtaccgcagtggcggaatacccaaccaagtcgcgtgcga
cgaaaggggttatctccatcaaggttaccgaacgtaacggtttagttgttggcgcggtacaggtagatgactgcgacca
gatcatgatgatcaccgatgccggtacgctggtacgtactcgcgtttcggaaatcagcatcgtgggccgtaacacccag
ggcgtgatcctcatccgtactgcggaagatgaaaacgtagtgggtctgcaacgtgttgctgaaccggttgacgaggaag
atctggataccatcgacggcagtgccgcggaaggggacgatgaaatcgctccggaagtggacgttgacgacgagccaga
agaagaataatactagatgtcgaactcttatgactcctccagtatcaaagtcctgaaagggctggatgcggtgcgtaag
cgcccgggtatgtatatcggcgacacggatgacggcaccggtctgcaccacatggtattcgaggtggtagataacgcta
tcgacgaagcgctcgcgggtcactgtaaagaaattatcgtcaccattcacgccgataactctgtctctgtacaggatga
cgggcgcggcattccgaccggtattcacccggaagagggcgtatcggcggcggaagtgatcatgaccgttctgcacgca
ggcggtaaatttgacgataactcctataaagtgtccggcggtctgcacggcgttggtgtttcggtagtaaacgccctgt
cgcaaaaactggagctggttatccagcgcgagggtaaaattcaccgtcagatctacgaacacggtgtaccgcaggcccc
gctggcggttaccggcgagactgaaaaaaccggcaccatggtgcgtttctggcccagcctcgaaaccttcaccaatgtg
accgagttcgaatatgaaattctggcgaaacgtctgcgtgagttgtcgttcctcaactccggcgtttccattcgtctgc
gcgacaagcgcgacggcaaagaagaccacttccactatgaaggcggcatcaaggcgttcgttgaatatctgaacaagaa
caaaacgccgatccacccgaatatcttctacttctccactgaaaaagacggtattggcgtcgaagtggcgttgcagtgg
aacgatggcttccaggaaaacatctactgctttaccaacaacattccgcagcgtgacggcggtactcacctggcaggct
tccgtgcggcgatgacccgtaccctgaacgcctacatggacaaagaaggctacagcaaaaaagccaaagtcagcgccac
cggtgacgatgcgcgtgaaggcctgattgcggtcgtttccgtgaaagtgccggacccgaaattctcctcccagaccaaa
gacaaactggtttcttctgaggtgaaatcggcggttgaacagcagatgaacgaactgctggcagaatacctgctggaaa
acccaaccgacgcgaaaatcgtggttggcaaaattatcgatgctgcccgtgcccgtgaagcggcgcgtcgcgcgcgtga
aatgacccgccgtaaaggtgcgctcgacttagcgggcctgccgggcaaactggcagactgccaggaacgcgatccggcg
ctttccgaactgtacctggtggaaggggactccgcgggcggctctgcgaagcaggggcgtaaccgcaagaaccaggcga
ttctgccgctgaagggtaaaatcctcaacgtcgagaaagcgcgcttcgataagatgctctcttctcaggaagtggcgac
gcttatcaccgcgcttggctgtggtatcggtcgtgacgagtacaacccggacaaactgcgttatcacagcatcatcatc
atgaccgatgcggacgtcgacggctcgcacattcgtacgctgctgttgaccttcttctatcgtcagatgccggaaatcg
ttgaacgcggtcacgtctacatcgctcagccgccgctgtacaaagtgaagaaaggcaagcaggaacagtacattaaaga
cgacgaagcgatggatcagtaccagatctctatcgcgctggacggcgcaacgctgcacaccaacgccagtgcaccggca
ttggctggcgaagcgttagagaaactggtatctgagtacaacgcgacgcagaaaatgatcaatcgtatggagcgtcgtt
atccgaaagcaatgctgaaagagcttatctatcagccgacgttgacggaagctgacctttctgatgagcagaccgttac
ccgctgggtgaacgcgctggtcagcgaactgaacgacaaagaacagcacggcagccagtggaagtttgatgttcacacc
aatgctgagcaaaacctgttcgagccgattgttcgcgtgcgtacccacggtgtggatactgactatccgctggatcacg
agtttatcaccggtggcgaatatcgtcgtatctgcacgctgggtgagaaactgcgtggcttgctggaagaagatgcgtt
tatcgaacgtggcgagcgtcgtcagccggtagccagcttcgagcaggcgctggactggctggtgaaagagtcccgtcgc
ggcctctccatccagcgttataaaggtctgggcgagatgaacccggaacagctgtgggaaaccactatggacccggaaa
gtcgtcgtatgctgcgcgttaccgttaaagatgcgattgctgccgaccagttgttcaccacgctgatgggcgacgccgt
tgaaccgcgccgtgcgtttattgaagagaacgccctgaaagcggcgaatatcgatatttaatactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata