LOCUS BBa_K676777 357 bp DNA linear 31-Oct-2024 DEFINITION GyrA cassette ACCESSION BBa_K676777 VERSION BBa_K676777.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 terminator 1..129 /label=BBa_B0015 CDS 138..225 /label=end of LacI coding region (inactive) misc_feature 138..337 /label=BBa_R0010 misc_feature 226..263 /label=CAP binding site promoter 274..279 /label=-35 promoter 298..303 /label=-10 misc_feature 303..337 /label=LacI binding site misc_feature 310..310 /label=start promoter 138..337 /label=BBa_R0010 misc_feature 350..353 /label=conserved RBS 346..357 /label=BBa_B0034 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 181 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 241 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 301 gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaa // LOCUS BBa_K185030 357 bp DNA linear 31-Oct-2024 DEFINITION dter-plac-rbs34 intermediate ACCESSION BBa_K185030 VERSION BBa_K185030.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 CDS 138..225 /label=end of LacI coding region (inactive) misc_feature 138..337 /label=BBa_R0010 misc_feature 226..263 /label=CAP binding site promoter 274..279 /label=-35 promoter 298..303 /label=-10 misc_feature 303..337 /label=LacI binding site misc_feature 310..310 /label=start promoter 138..337 /label=BBa_R0010 misc_feature 350..353 /label=conserved RBS 346..357 /label=BBa_B0034 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 181 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 241 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 301 gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaa //