LOCUS       BBa_K678002             3398 bp    DNA     linear       22-Dec-2024
DEFINITION  eGFP-PTS1 for mammalian cells
ACCESSION   BBa_K678002
VERSION     BBa_K678002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..8
                     /label=Linker 1
     promoter        9..662
                     /label=CMV
     misc_feature    663..671
                     /label=Linker 2
     CDS             679..1394
                     /label=eGFP
     misc_feature    672..682
                     /label=Kozak
     misc_feature    1395..1406
                     /label=PTS1
     misc_feature    1407..1414
                     /label=Linker 4
     polyA_site      1415..1639
                     /label=BGH pA
     misc_feature    1640..1647
                     /label=Linker 5
     misc_feature    1648..3398
                     /label=Hygromycin cassette
     misc_feature    3399..3406
                     /label=Linker 6
ORIGIN
        1 acgtcgctcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
       61 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      121 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      181 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      241 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      301 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      361 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      421 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      481 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      541 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      601 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
      661 atagtgcgat cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      721 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      781 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      841 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
      901 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      961 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1021 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1081 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     1141 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     1201 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1261 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     1321 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1381 acgagctgta caagtccaag ctgtaaattc cgatctgtgc cttctagttg ccagccatct
     1441 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     1501 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     1561 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     1621 gatgcggtgg gctctatgga cttgcgtcca gcaggcagaa gtatgcaaag catgcatctc
     1681 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     1741 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     1801 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     1861 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     1921 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     1981 cagcacgtga tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     2041 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     2101 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     2161 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     2221 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     2281 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     2341 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     2401 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     2461 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     2521 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     2581 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     2641 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     2701 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     2761 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     2821 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     2881 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     2941 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     3001 agggcaaagg aatagcacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     3061 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     3121 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     3181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3241 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     3301 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3361 cacaacatac gagccggaag cataaagtgt aaagcctg
//