LOCUS BBa_K678049 6092 bp DNA linear 17-Dec-2024 DEFINITION pJEJAM1 ACCESSION BBa_K678049 VERSION BBa_K678049.1 FEATURES Location/Qualifiers promoter 1..654 /label=CMV+RBS promoter 1..654 /label=BBa_J52034 misc_feature 655..662 /label=Linker 2 CDS 670..1389 /label=eGFP misc_feature 663..673 /label=Kozak misc_feature 655..1389 /label=BBa_K678006 misc_feature 1390..1397 /label=Linker 4 polyA_site 1398..1622 /label=BGH terminator 1390..1622 /label=BBa_K678019 misc_feature 1623..1630 /label=Linker 5 misc_feature 1631..3381 /label=Hygromycin cassette misc_feature 1623..3381 /label=BBa_K678021 misc_feature 3382..3389 /label=Linker misc_feature 3390..6092 /label=Plasmid Mam misc_feature 3382..6092 /label=BBa_K678023 ORIGIN 1 cgatgtacgg gccagatata cgcgttgaca ttgattattg cctagttatt aatagtaatc 61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaatagtgcg 661 atcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 721 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 781 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 841 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac 901 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 961 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 1021 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 1081 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 1141 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 1201 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 1261 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 1321 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 1381 tacaagtaaa ttccgatctg tgccttctag ttgccagcca tctgttgttt gcccctcccc 1441 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 1501 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 1561 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 1621 ggacttgcgt ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 1681 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 1741 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 1801 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 1861 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 1921 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgatgaaaaa 1981 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc 2041 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg 2101 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt 2161 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt 2221 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct 2281 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc 2341 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca 2401 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca 2461 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct 2521 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa 2581 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg 2641 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga 2701 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg 2761 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt 2821 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac 2881 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga 2941 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca 3001 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 3061 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 3121 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 3181 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 3241 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 3301 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 3361 aagcataaag tgtaaagcct gattaagcta gtgagtcgaa taagggcgac acaaaattta 3421 ttctaaatgc ataataaata ctgataacat cttatagttt gtattatatt ttgtattatc 3481 gttgacatgt ataattttga tatcaaaaac tgattttccc tttattattt tcgagattta 3541 ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatc ataaataata 3601 gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt 3661 gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag 3721 gcgcccttaa atattctgac aaatgctctt tccctaaact ccccccataa aaaaacccgc 3781 cgaagcgggt ttttacgtta tttgcggatt aacgattact cgttatcaga accgcccagg 3841 gggcccgagc ttaagactgg ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa 3901 aaaggccatc cgtcaggggc cttctgctta gtttgatgcc tggcagttcc ctactctcgc 3961 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 4021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 4081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 4141 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 4201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 4261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 4321 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 4381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 4441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 4501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggg 4561 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 4621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 4681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 4741 tgatcttttc tacggggtct gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg 4801 gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg cttttaccaa 4861 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 4921 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagcgct 4981 gcgatgatac cgcgagaacc acgctcaccg gctccggatt tatcagcaat aaaccagcca 5041 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 5101 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 5161 gccatcgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 5221 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 5281 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 5341 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 5401 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 5461 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 5521 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 5581 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 5641 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 5701 tgttgaatac tcatattctt cctttttcaa tattattgaa gcatttatca gggttattgt 5761 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggtcagtgtt 5821 acaaccaatt aaccaattct gaacattatc gcgagcccat ttatacctga atatggctca 5881 taacacccct tgtttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa 5941 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg actccccatg cgagagtagg 6001 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgcccgg 6061 gctaattatg gggtgtcgcc cttattcgac tc //