LOCUS BBa_K678050 6101 bp DNA linear 17-Dec-2024 DEFINITION pJEJAM2 ACCESSION BBa_K678050 VERSION BBa_K678050.1 FEATURES Location/Qualifiers promoter 1..654 /label=CMV+RBS promoter 1..654 /label=BBa_J52034 CDS 670..1371 /label=eGFP misc_feature 1366..1398 /label=PTS misc_feature 655..662 /label=Linker 2 misc_feature 663..673 /label=Kozak misc_feature 655..1398 /label=BBa_K678017 misc_feature 1399..1406 /label=Linker 4 polyA_site 1407..1631 /label=BGH terminator 1399..1631 /label=BBa_K678019 misc_feature 1632..1639 /label=Linker 5 misc_feature 1640..3390 /label=Hygromycin cassette misc_feature 1632..3390 /label=BBa_K678021 misc_feature 3391..3398 /label=Linker misc_feature 3399..6101 /label=Plasmid Mam misc_feature 3391..6101 /label=BBa_K678023 ORIGIN 1 cgatgtacgg gccagatata cgcgttgaca ttgattattg cctagttatt aatagtaatc 61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaatagtgcg 661 atcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 721 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 781 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 841 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac 901 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 961 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 1021 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 1081 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 1141 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 1201 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 1261 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 1321 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 1381 tacaagtcca agctgtaaat tccgatctgt gccttctagt tgccagccat ctgttgtttg 1441 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 1501 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 1561 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 1621 gggctctatg gacttgcgtc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 1681 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 1741 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 1801 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 1861 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 1921 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt 1981 gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga 2041 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga 2101 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga 2161 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat 2221 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt 2281 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga 2341 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg 2401 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta 2461 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga 2521 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg 2581 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc 2641 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc 2701 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc 2761 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga 2821 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg 2881 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg 2941 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa 3001 ggaatagcac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 3061 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 3121 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag 3181 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 3241 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta 3301 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 3361 acgagccgga agcataaagt gtaaagcctg attaagctag tgagtcgaat aagggcgaca 3421 caaaatttat tctaaatgca taataaatac tgataacatc ttatagtttg tattatattt 3481 tgtattatcg ttgacatgta taattttgat atcaaaaact gattttccct ttattatttt 3541 cgagatttat tttcttaatt ctctttaaca aactagaaat attgtatata caaaaaatca 3601 taaataatag atgaatagtt taattatagg tgttcatcaa tcgaaaaagc aacgtatctt 3661 atttaaagtg cgttgctttt ttctcattta taaggttaaa taattctcat atatcaagca 3721 aagtgacagg cgcccttaaa tattctgaca aatgctcttt ccctaaactc cccccataaa 3781 aaaacccgcc gaagcgggtt tttacgttat ttgcggatta acgattactc gttatcagaa 3841 ccgcccaggg ggcccgagct taagactggc cgtcgtttta caacacagaa agagtttgta 3901 gaaacgcaaa aaggccatcc gtcaggggcc ttctgcttag tttgatgcct ggcagttccc 3961 tactctcgcc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 4021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 4081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 4141 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 4201 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 4261 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 4321 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 4381 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 4441 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 4501 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 4561 agtggtgggc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 4621 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 4681 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 4741 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg cgcgtaactc 4801 acgttaaggg attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc 4861 ttttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 4921 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 4981 cccagcgctg cgatgatacc gcgagaacca cgctcaccgg ctccggattt atcagcaata 5041 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 5101 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 5161 aacgttgttg ccatcgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 5221 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 5281 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 5341 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 5401 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 5461 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 5521 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 5581 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 5641 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 5701 acacggaaat gttgaatact catattcttc ctttttcaat attattgaag catttatcag 5761 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 5821 gtcagtgtta caaccaatta accaattctg aacattatcg cgagcccatt tatacctgaa 5881 tatggctcat aacacccctt gtttgcctgg cggcagtagc gcggtggtcc cacctgaccc 5941 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtgggga ctccccatgc 6001 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 6061 ttcgcccggg ctaattatgg ggtgtcgccc ttattcgact c //