LOCUS BBa_K678051 6170 bp DNA linear 15-Nov-2024 DEFINITION pJEJAM3 ACCESSION BBa_K678051 VERSION BBa_K678051.1 FEATURES Location/Qualifiers promoter 1..654 /label=CMV+RBS promoter 1..654 /label=BBa_J52034 misc_feature 655..662 /label=Linker 2 CDS 670..1467 /label=eYFP-module misc_feature 663..671 /label=Kozak misc_feature 655..1467 /label=BBa_K678009 misc_feature 1468..1475 /label=Linker 4 polyA_site 1476..1700 /label=BGH terminator 1468..1700 /label=BBa_K678019 misc_feature 1701..1708 /label=Linker 5 misc_feature 1709..3459 /label=Hygromycin cassette misc_feature 1701..3459 /label=BBa_K678021 misc_feature 3460..3467 /label=Linker misc_feature 3468..6170 /label=Plasmid Mam misc_feature 3460..6170 /label=BBa_K678023 ORIGIN 1 cgatgtacgg gccagatata cgcgttgaca ttgattattg cctagttatt aatagtaatc 61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaatagtgcg 661 atcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 721 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 781 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 841 tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac 901 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 961 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 1021 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 1081 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 1141 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 1201 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 1261 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 1321 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 1381 tacaagtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg 1441 ggcccgggat ccaccggatc tagataaatt ccgatctgtg ccttctagtt gccagccatc 1501 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 1561 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 1621 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 1681 ggatgcggtg ggctctatgg acttgcgtcc agcaggcaga agtatgcaaa gcatgcatct 1741 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 1801 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 1861 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 1921 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 1981 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 2041 tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga 2101 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt 2161 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt 2221 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt 2281 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg 2341 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga 2401 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg 2461 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc 2521 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc 2581 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 2641 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 2701 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 2761 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 2821 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 2881 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 2941 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 3001 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 3061 gagggcaaag gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag 3121 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 3181 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata 3241 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 3301 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag 3361 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 3421 acacaacata cgagccggaa gcataaagtg taaagcctga ttaagctagt gagtcgaata 3481 agggcgacac aaaatttatt ctaaatgcat aataaatact gataacatct tatagtttgt 3541 attatatttt gtattatcgt tgacatgtat aattttgata tcaaaaactg attttccctt 3601 tattattttc gagatttatt ttcttaattc tctttaacaa actagaaata ttgtatatac 3661 aaaaaatcat aaataataga tgaatagttt aattataggt gttcatcaat cgaaaaagca 3721 acgtatctta tttaaagtgc gttgcttttt tctcatttat aaggttaaat aattctcata 3781 tatcaagcaa agtgacaggc gcccttaaat attctgacaa atgctctttc cctaaactcc 3841 ccccataaaa aaacccgccg aagcgggttt ttacgttatt tgcggattaa cgattactcg 3901 ttatcagaac cgcccagggg gcccgagctt aagactggcc gtcgttttac aacacagaaa 3961 gagtttgtag aaacgcaaaa aggccatccg tcaggggcct tctgcttagt ttgatgcctg 4021 gcagttccct actctcgcct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 4081 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 4141 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 4201 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 4261 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 4321 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 4381 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 4441 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 4501 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 4561 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 4621 agttcttgaa gtggtgggct aactacggct acactagaag aacagtattt ggtatctgcg 4681 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 4741 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 4801 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgacgcgc 4861 gcgtaactca cgttaaggga ttttggtcat gagcttgcgc cgtcccgtca agtcagcgta 4921 atgctctgct tttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 4981 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 5041 ccatctggcc ccagcgctgc gatgataccg cgagaaccac gctcaccggc tccggattta 5101 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 5161 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 5221 agtttgcgca acgttgttgc catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 5281 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 5341 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 5401 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 5461 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 5521 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 5581 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 5641 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 5701 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 5761 ataagggcga cacggaaatg ttgaatactc atattcttcc tttttcaata ttattgaagc 5821 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 5881 caaatagggg tcagtgttac aaccaattaa ccaattctga acattatcgc gagcccattt 5941 atacctgaat atggctcata acaccccttg tttgcctggc ggcagtagcg cggtggtccc 6001 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggac 6061 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag 6121 actgggcctt tcgcccgggc taattatggg gtgtcgccct tattcgactc //