LOCUS BBa_K678053 6083 bp DNA linear 15-Nov-2024 DEFINITION pJEJAM5 ACCESSION BBa_K678053 VERSION BBa_K678053.1 FEATURES Location/Qualifiers promoter 1..654 /label=CMV+RBS promoter 1..654 /label=BBa_J52034 misc_feature 655..662 /label=Linker 2 CDS 670..1380 /label=mCherry misc_feature 663..673 /label=Kozak misc_feature 655..1380 /label=BBa_K678020 misc_feature 1381..1388 /label=Linker 4 polyA_site 1389..1613 /label=BGH terminator 1381..1613 /label=BBa_K678019 misc_feature 1614..1621 /label=Linker 5 misc_feature 1622..3372 /label=Hygromycin cassette misc_feature 1614..3372 /label=BBa_K678021 misc_feature 3373..3380 /label=Linker misc_feature 3381..6083 /label=Plasmid Mam misc_feature 3373..6083 /label=BBa_K678023 ORIGIN 1 cgatgtacgg gccagatata cgcgttgaca ttgattattg cctagttatt aatagtaatc 61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaatagtgcg 661 atcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg 721 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag 781 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc 841 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac 901 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag 961 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc 1021 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac 1081 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc 1141 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac 1201 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc 1261 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa 1321 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtag 1381 attccgatct gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 1441 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 1501 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 1561 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggacttgcg 1621 tccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 1681 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 1741 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 1801 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 1861 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 1921 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgatgaaaa agcctgaact 1981 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat 2041 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata 2101 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca 2161 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag 2221 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac 2281 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga 2341 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac 2401 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat 2461 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga 2521 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac 2581 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca 2641 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac 2701 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat 2761 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc 2821 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg 2881 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc 2941 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtgctacg 3001 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 3061 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa 3121 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 3181 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 3241 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt 3301 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 3361 gtgtaaagcc tgattaagct agtgagtcga ataagggcga cacaaaattt attctaaatg 3421 cataataaat actgataaca tcttatagtt tgtattatat tttgtattat cgttgacatg 3481 tataattttg atatcaaaaa ctgattttcc ctttattatt ttcgagattt attttcttaa 3541 ttctctttaa caaactagaa atattgtata tacaaaaaat cataaataat agatgaatag 3601 tttaattata ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt 3661 ttttctcatt tataaggtta aataattctc atatatcaag caaagtgaca ggcgccctta 3721 aatattctga caaatgctct ttccctaaac tccccccata aaaaaacccg ccgaagcggg 3781 tttttacgtt atttgcggat taacgattac tcgttatcag aaccgcccag ggggcccgag 3841 cttaagactg gccgtcgttt tacaacacag aaagagtttg tagaaacgca aaaaggccat 3901 ccgtcagggg ccttctgctt agtttgatgc ctggcagttc cctactctcg ccttccgctt 3961 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 4021 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 4081 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 4141 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 4201 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 4261 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 4321 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 4381 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 4441 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 4501 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg gctaactacg 4561 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 4621 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 4681 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 4741 ctacggggtc tgacgctcag tggaacgacg cgcgcgtaac tcacgttaag ggattttggt 4801 catgagcttg cgccgtcccg tcaagtcagc gtaatgctct gcttttacca atgcttaatc 4861 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 4921 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagcgc tgcgatgata 4981 ccgcgagaac cacgctcacc ggctccggat ttatcagcaa taaaccagcc agccggaagg 5041 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 5101 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct 5161 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 5221 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 5281 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 5341 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 5401 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 5461 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 5521 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 5581 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 5641 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 5701 ctcatattct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 5761 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggtcagtgt tacaaccaat 5821 taaccaattc tgaacattat cgcgagccca tttatacctg aatatggctc ataacacccc 5881 ttgtttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt 5941 gaaacgccgt agcgccgatg gtagtgtggg gactccccat gcgagagtag ggaactgcca 6001 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgcccg ggctaattat 6061 ggggtgtcgc ccttattcga ctc //