LOCUS BBa_K678054 4054 bp DNA linear 15-Nov-2024 DEFINITION pJEJAM6 ACCESSION BBa_K678054 VERSION BBa_K678054.1 FEATURES Location/Qualifiers misc_feature 1..8 /label=Linker 1 promoter 9..407 /label=PGK promoter 1..407 /label=BBa_K678004 misc_feature 416..423 /label=Linker 2 misc_feature 424..434 /label=Kozak CDS 431..1147 /label=eGFP+K GOI misc_feature 416..1147 /label=BBa_K678007 misc_feature 1156..1164 /label=Linker 3 misc_feature 1164..1174 /label=Kozak CDS 1171..1891 /label=eGFP-TS misc_feature 1156..1891 /label=BBa_K678008 misc_feature 1900..1907 /label=Linker 4 polyA_site 1908..2534 /label=hGH terminator 1900..2534 /label=BBa_K678018 misc_feature 2543..2550 /label=Linker 5 misc_feature 2551..4054 /label=Neomycin cassette misc_feature 2543..4054 /label=BBa_K678022 ORIGIN 1 acgtcgctcc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc 61 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg 121 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg 181 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc 241 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc 301 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc 361 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcctac tagagagtgc 421 gatcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 481 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 541 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 601 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga 661 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 721 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 781 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 841 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 901 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 961 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 1021 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 1081 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 1141 gtacaagtac tagagagcgc tggtcgccac catggtgagc aagggcgagg agctgttcac 1201 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 1261 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 1321 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca 1381 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 1441 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 1501 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 1561 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 1621 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 1681 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 1741 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 1801 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 1861 cactctcggc atggacgagc tgtacaagta atactagaga ttccgatagg atcccgggtg 1921 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc 1981 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta 2041 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg 2101 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac 2161 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg 2221 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt 2281 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc 2341 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt 2401 aaaataacta taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca 2461 accggtggga catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca 2521 gtagatgcct gttgtactag agacttgcgt ctgtggaatg tgtgtcagtt agggtgtgga 2581 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 2641 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 2701 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 2761 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 2821 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 2881 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga 2941 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 3001 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 3061 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 3121 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 3181 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 3241 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 3301 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 3361 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 3421 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 3481 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 3541 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 3601 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 3661 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 3721 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 3781 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 3841 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 3901 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa 3961 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 4021 tgtccaaact catcaatgta tcttatcatg tctg //