LOCUS BBa_K678056 3485 bp DNA linear 17-Dec-2024 DEFINITION pJEJAM8 ACCESSION BBa_K678056 VERSION BBa_K678056.1 FEATURES Location/Qualifiers promoter 9..352 /label=SV40 + ori misc_feature 1..8 /label=Linker 1 promoter 1..352 /label=BBa_K678005 misc_feature 361..368 /label=Linker 2 CDS 376..1083 /label=mCherry-GOI misc_feature 369..379 /label=Kozak misc_feature 361..1083 /label=BBa_K678013 misc_feature 1092..1100 /label=Linker 3 CDS 1108..1818 /label=mCherry-TS misc_feature 1101..1111 /label=misc misc_feature 1092..1818 /label=BBa_K678014 misc_feature 1827..1834 /label=Linker 4 polyA_site 1835..1965 /label=SV40 terminator 1827..1965 /label=BBa_K678012 misc_feature 1974..1981 /label=Linker 5 misc_feature 1982..3485 /label=Neomycin cassette misc_feature 1974..3485 /label=BBa_K678022 ORIGIN 1 acgtcgctct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttactagag 361 agtgcgatcg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag 421 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag 481 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag 541 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag 601 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc 661 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac 721 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc 781 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg 841 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc 901 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc 961 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc 1021 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac 1081 aagtactaga gagcgctggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc 1141 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag 1201 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 1261 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg 1321 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc 1381 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc 1441 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc 1501 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 1561 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag 1621 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc 1681 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag 1741 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg 1801 gacgagctgt acaagtagta ctagagattc cgataacttg tttattgcag cttataatgg 1861 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 1921 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtacta gagacttgcg 1981 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 2041 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 2101 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 2161 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 2221 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 2281 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 2341 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 2401 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 2461 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 2521 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 2581 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 2641 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 2701 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 2761 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 2821 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 2881 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 2941 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 3001 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 3061 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 3121 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 3181 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 3241 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 3301 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg 3361 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 3421 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 3481 gtctg //