LOCUS BBa_K678057 3659 bp DNA linear 17-Dec-2024 DEFINITION pJEJAM9 ACCESSION BBa_K678057 VERSION BBa_K678057.1 FEATURES Location/Qualifiers promoter 9..352 /label=SV40 + ori misc_feature 1..8 /label=Linker 1 promoter 1..352 /label=BBa_K678005 misc_feature 361..368 /label=Linker 2 CDS 376..1162 /label=eCFP-GOI misc_feature 369..379 /label=Kosaz misc_feature 361..1170 /label=BBa_K678048 misc_feature 1179..1187 /label=Linker 3 CDS 1195..1992 /label=eCFP-TS misc_feature 1188..1198 /label=Kozak misc_feature 1179..1992 /label=BBa_K678016 misc_feature 2001..2008 /label=Linker 4 polyA_site 2009..2139 /label=SV40 terminator 2001..2139 /label=BBa_K678012 misc_feature 2148..2155 /label=Linker 5 misc_feature 2156..3659 /label=Neomycin cassette misc_feature 2148..3659 /label=BBa_K678022 ORIGIN 1 acgtcgctct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttactagag 361 agtgcgatcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 421 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 481 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 541 gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac 601 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 661 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 721 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 781 aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc 841 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc 901 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 961 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag 1021 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 1081 gagctgtaca agtccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt 1141 accgcgggcc cgggatccac cggatctaga tactagagag cgctggtcgc caccatggtg 1201 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 1261 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 1321 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 1381 accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 1441 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 1501 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 1561 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 1621 gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc 1681 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 1741 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 1801 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 1861 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc 1921 agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc 1981 ggatctagat aatactagag attccgataa cttgtttatt gcagcttata atggttacaa 2041 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 2101 tggtttgtcc aaactcatca atgtatctta tcatgtctgt actagagact tgcgtctgtg 2161 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 2221 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 2281 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 2341 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 2401 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 2461 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 2521 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 2581 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 2641 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 2701 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 2761 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 2821 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 2881 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 2941 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 3001 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 3061 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 3121 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 3181 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 3241 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 3301 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 3361 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 3421 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 3481 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 3541 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 3601 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctg //