LOCUS BBa_K678058 7121 bp DNA linear 15-Nov-2024 DEFINITION pJEJAM10 ACCESSION BBa_K678058 VERSION BBa_K678058.1 FEATURES Location/Qualifiers misc_feature 844..851 /label=Linker 2 misc_feature 1..8 /label=Linker 1 promoter 9..843 /label=PalcA promoter 1..843 /label=BBa_K678001 misc_feature 852..859 /label=Linker 2 CDS 860..3934 /label=lacZ misc_feature 852..3934 /label=BBa_K678026 misc_feature 3951..4948 /label=T1-motni terminator misc_feature 3943..3950 /label=Linker 4 terminator 3943..4948 /label=BBa_K678037 misc_feature 4957..4964 /label=Linker 5 misc_feature 4965..7121 /label=argB cassette misc_feature 4957..7121 /label=BBa_K678043 ORIGIN 1 acgtcgctct ccccgatgac atacaggagg ggtcagggca ttcgagaaat accgtggata 61 cagttgggca tttctagggc tgaatgggaa ggagagagtt ttgaaatagg cgttccgttc 121 tgcttagggt atttgggaac aatcaatgtt caatgtacat ttaatccacg attttataaa 181 acgtcatcct ttgccctccc ttcttatttg ccaataccaa aaatcttact ccagtggttc 241 ggtaatcgca gagttaaatc tgggctcggt ggcagatctg cgatgctcca taaccgttca 301 gatgttgatt ggaactgggt ggggtagaca gctcgaagac cgagtgaacg tatacctaag 361 acactttgac acggccggaa cactgtaagt cccttcgtat ttctccgcct gtgtggagct 421 accatccaat aacccccagc tgaaaagctg attgtgatag ttcccacttg tccgtccgca 481 tcggcatccg cagctcggga tagttccgac ctaggattgg atgcatgcgg aaccgcacga 541 gggcggggcg gaaattgaca caccactcct ctccacgcac cgttcaagag gtacgcgtat 601 agagccgtat agagcagaga cggagcactt tctggtactg tccgcacggg atgtccgcac 661 ggagagccac aaacgagcgg ggccccgtac gtgctctcct accccaggat cgcatccccg 721 catagctgaa catctatata aagaccccca aggttctcag tctcaccaac atcatcaacc 781 aacaatcaac agttctctac tcagttaatt agaactcttc caatcctatc acctcgcctc 841 aaatactaga gagtgcgata tgaccatgat tacggattca ctggccgtcg ttttacaacg 901 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 961 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag 1021 cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg 1081 gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca 1141 cggttacgat gcgcccatct acaccaacgt gacctatccc attacggtca atccgccgtt 1201 tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg 1261 gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg 1321 gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct 1381 gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gctggagtga 1441 cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc 1501 gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga 1561 tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta 1621 cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc 1681 tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct 1741 gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt 1801 tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg 1861 cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg 1921 cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt 1981 gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc 2041 gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc 2101 caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct 2161 accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt 2221 gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg 2281 ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac 2341 cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc 2401 ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct 2461 gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg 2521 gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca 2581 gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg 2641 cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc 2701 gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg 2761 gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca 2821 ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc 2881 tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca 2941 actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccggaca 3001 catcagcgcc tggcagcagt ggcgtctggc tgaaaacctc agcgtgacac tccccgccgc 3061 gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa 3121 taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa 3181 aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat 3241 tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc 3301 gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc 3361 ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg 3421 gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc 3481 gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga 3541 gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc 3601 ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag 3661 cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg 3721 cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg 3781 ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat 3841 tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttccagctga gcgccggtcg 3901 ctaccattac cagttggtct ggtgtcaaaa ataatactag agattccgat ggctccgagg 3961 ctactggagt ggctgcaact gctcattgga aaaggatatg aggtgcgggg ggtcaaaccg 4021 attgggcccg gtagctcatt acttgactga cggtctcatc tatctggccc tgcgcaaacc 4081 tcactagttc ttcgctaagt cggagagaca gcgactagat tcggacgaag tgactttccc 4141 cccggaaccg ccaacctact ggacaacaaa ctactctagt gacttccagg tttaggttca 4201 aatcatcagt gcggcatttc ggtggtctag aactgggagc aacggtgcaa ttggccctag 4261 taacaggcaa gtaattctca tccccgctag cattcgctcc gtgtgcggac acaatagcta 4321 ttgcgcactg tatgtagact gtcacgcttg tcaaccttcg ccgctgcatc ttactaaaga 4381 gctaccaacc aacacatgca gtagctcgtt gttgatacgc cgtacattct tggctcttgc 4441 gtatatatac atttgtgatt cagatttacc ctgcctcgca cattgcttct ttgctcatct 4501 tcccttccct aagtcgacat ggtgaaataa tgtttgaggt ggcgcctgca attacatact 4561 catcatatta aatgggcttg ccgcgggaaa gacgtaaacg ccactatggg tttgctaaca 4621 aattgattga cagagttgcc gttgaaggcc tggtctaatg caggcccgca ctatgagacg 4681 cggcagacgc agccactcta gtcttcaatt ccaacaattg aggatgtggc catgccaagt 4741 cactcatgca taaactatgc tggccagtcc gggctcacgg agcttcgagc accaatcgcc 4801 gtccagacag atagcatgtc ttcagatcca gtccctgctt cgttatctgc atgcgtgtgc 4861 attgacaatg ctgttctttg ccctcagaat atttcccaat acacattgtt tgcctctcac 4921 tttcgagtat attggcatca gacgtcgcta ctagagactt gcgtcgcggt tttttggggt 4981 agtcatctaa tgaaacagac ccggacgcag cagaggaagc cccgcgatga ctctatacca 5041 ccgtacgccg atatatcatc atcgcggcga tggagaagtg gggttgactc cgaagacact 5101 tcaaaggagc gacgctgttg atttgtagac gacgcttgat agggagaagc attattgtcg 5161 tgatgctcgc ccaacagagg ccgactcgcc tcatccgtca taacgaacgc tgtgtaaagc 5221 ggagtggggg ggaaagtgtg gattgtggag agtatgcgat agtgttgagg ctgatcagac 5281 ggcgaatcgg gccagatatg accagtttag aggcctcatt tgactataat ttacataaat 5341 tagataaata gagatgaacg catgcaataa ttgcagcaaa tattgatgaa gcgagaggta 5401 ggacgatgaa ggactgtgag cagttcaagg tatcagcaga gtcaagggcc tgatgcaatg 5461 gcggtgatcc gtgatcagcg aacggaaggg gcgctaactc tgtttcttta ccaatgatcg 5521 gaagctcctg ctggcggact tatgagtcat tcacgaatca tttctcagtt atttgtggat 5581 gccctcgttc tgtccacaat ttctttccgc cccaagtctt ttaagttctt taacatctat 5641 attcttgcac ttccaatggc atcccttcgc tccgtactca agagccagag cttgcgacac 5701 accgtgcgat cctactcctc gcaaaccatg cctcccgcct caccctttgc tccccgccac 5761 ttcctctcca ttgcggacct ctcgccctcc gagttcgcaa cccttgttcg caatgcctcc 5821 tcacacaaac gggctatcaa gtcggggtca atgccccaaa acttgcaggg atcactcctt 5881 gggaaaactg tggccatgat cttcagcaaa cgaagcacga ggacaagggt atctacagaa 5941 ggggccgttg tgcagatggg aggtcatccg atgttcttgg gcaaggatga tatccaacta 6001 ggtgtcaacg agtccctata cgacacctcc gttgtcattt cgtccatggt atcctgcatt 6061 gtagcccgtg tcggtaaaca tgcagaggtc gcagatctgg cgaagcactc ttcggttcca 6121 gtcatcaatg ctttgtgtga ctctttccac cctctccaag ccgtggccga tttccagacc 6181 atctatgaag cattcacccc caaggcgcac cacctttcaa gtctagggtt ggaaggattg 6241 aagatcgctt gggtgggtga cgccaacaac gtcctgttcg atatggccat tgctgctaca 6301 aaaatgggtg tcgacattgc tgtcgctact cccaaggggt acgaaatccc tcctcacatg 6361 ctggagctca tcaagtctgc tggagagggt gtctcgaaac caggaaagct tctgcaaacc 6421 aatattcccg aagaagcggt caaggacgcc gatattctgg tcacagacac ctgggtctct 6481 atgggccaag aggaagagaa ggctcagagg ctgaaggagt ttgatggttt ccaaatcact 6541 gctgaactcg ccaagcgagg aggagctaag gagggctgga agttcatgca ctgtctcccg 6601 cgacaccctg aggaggtcag cgacgaggtt ttctacagca accggtcact tgtcttccct 6661 gaggctgaga accggttatg ggctgcgatt tccgccttgg agggtttcgt tgtcaataag 6721 ggaaaaattg aataaatata accaggcttc catttaaata tatagaggct ggcgttatca 6781 aactgatgag ttgacgggta tgagatcatt ccgtccctaa atatatttac tccgatcacg 6841 taaaagcctg ttagtagaag cattttccca attatcctga ccaattcttt ctagcatata 6901 tcaaataact aattgacatg ttctctcgct tccttatatt actcagagta ttggaaatgg 6961 ggcaaatcgc acccggtgac tttcacatgt cacgaatgcg gagtcgtcct agccaaggta 7021 gatccaggcc taacacaccc caaccctcga ctctcctcat tccatactct tgacctctat 7081 ccagcacatt cttctgaggt ttcgcaatgg ctgtaggtgg c //