LOCUS BBa_K678060 8133 bp DNA linear 15-Nov-2024 DEFINITION pJEJAM12 ACCESSION BBa_K678060 VERSION BBa_K678060.1 FEATURES Location/Qualifiers misc_feature 1..8 /label=Linker 1 promoter 9..2312 /label=PgpdA promoter 1..2312 /label=BBa_K678024 misc_feature 2313..2320 /label=Linker 2 CDS 2321..3040 /label=GFP misc_feature 2313..3040 /label=BBa_K678027 misc_feature 3041..3048 /label=Linker 4 misc_feature 3049..3799 /label=TtrpC terminator terminator 3041..3799 /label=BBa_K678036 misc_feature 3800..3807 /label=Linker 5 misc_feature 3808..5255 /label=pyrG-cassette CDS 3800..5255 /label=BBa_K678040 misc_feature 5256..5263 /label=Linker 6 misc_feature 5264..8133 /label=pFun misc_feature 5256..8133 /label=BBa_K678046 ORIGIN 1 acgtcgctat tcccttgtat ctctacacac aggctcaaat caataagaag aacggttcgt 61 ctttttcgtt tatatcttgc atcgtcccaa agctattggc gggatattct gtttgcagtt 121 ggctgacttg aagtaatctc tgcagatctt tcgacactga aatacgtcga gcctgctccg 181 cttggaagcg gcgaggagcc tcgtcctgtc acaactacca acatggagta cgataagggc 241 cagttccgcc agctcattaa gagccagttc atgggcgttg gcatgatggc cgtcatgcat 301 ctgtacttca agtacaccaa cgctcttctg atccagtcga tcatccgctg aaggcgcttt 361 cgaatctggt taagatccac gtcttcggga agccagcgac tggtgacctc cagcgtccct 421 ttaaggctgc caacagcttt ctcagccagg gccagcccaa gaccgacaag gcctccctcc 481 agaacgccga gaagaactgg aggggtggtg tcaaggagga gtaagctcct tattgaagtc 541 ggaggacgga gcggtgtcaa gaggatattc ttcgactctg tattatagat aagatgatga 601 ggaattggag gtagcatagc ttcatttgga tttgctttcc aggctgagac tctagcttgg 661 agcatagagg gtcctttggc tttcaatatt ctcaagtatc tcgagtttga acttattccc 721 tgtgaacctt ttattcacca atgagcattg gaatgaacat gaatctgagg actgcaatcg 781 ccatgaggtt ttcgaaatac atccggatgt cgaaggcttg gggcacctgc gttggttgaa 841 tttagaacgt ggcactattg atcatccgat agctctgcaa agggcgttgc acaatgcaag 901 tcaaacgttg ctagcagttc caggtggaat gttatgatga gcattgtatt aaatcaggag 961 atatagcatg atctctagtt agctcaccac aaaagtcaga cggcgtaacc aaaagtcaca 1021 caacacaagc tgtaaggatt tcggcacggc tacggaagac ggagaagcca ccttcagtgg 1081 actcgagtac catttaattc tatttgtgtt tgatcgagac ctaatacagc ccctacaacg 1141 accatcaaag tcgtatagct accagtgagg aagtggactc aaatcgactt cagcaacatc 1201 tcctggataa actttaagcc taaactatac agaataagat aggtggagag cttataccga 1261 gctcccaaat ctgtccagat catggttgac cggtgcctgg atcttcctat agaatcatcc 1321 ttattcgttg acctagctga ttctggagtg acccagaggg tcatgacttg agcctaaaat 1381 ccgccgcctc caccatttgt agaaaaatgt gacgaactcg tgagctctgt acagtgaccg 1441 gtgactcttt ctggcatgcg gagagacgga cggacgcaga gagaagggct gagtaataag 1501 ccactggcca gacagctctg gcggctctga ggtgcagtgg atgattatta atccgggacc 1561 ggccgcccct ccgccccgaa gtggaaaggc tggtgtgccc ctcgttgacc aagaatctat 1621 tgcatcatcg gagaatatgg agcttcatcg aatcaccggc agtaagcgaa ggagaatgtg 1681 aagccagggg tgtatagccg tcggcgaaat agcatgccat taacctaggt acagaagtcc 1741 aattgcttcc gatctggtaa aagattcacg agatagtacc ttctccgaag taggtagagc 1801 gagtacccgg cgcgtaagct ccctaattgg cccatccggc atctgtaggg cgtccaaata 1861 tcgtgcctct cctgctttgc ccggtgtatg aaaccggaaa ggccgctcag gagctggcca 1921 gcggcgcaga ccgggaacac aagctggcag tcgacccatc cggtgctctg cactcgacct 1981 gctgaggtcc ctcagtccct ggtaggcagc tttgccccgt ctgtccgccc ggtgtgtcgg 2041 cggggttgac aaggtcgttg cgtcagtcca acatttgttg ccatattttc ctgctctccc 2101 caccagctgc tcttttcttt tctctttctt ttcccatctt cagtatattc atcttcccat 2161 ccaagaacct ttatttcccc taagtaagta ctttgctaca tccatactcc atccttccca 2221 tcccttattc ctttgaacct ttcagttcga gctttcccac ttcatcgcag cttgactaac 2281 agctaccccg cttgagcaga catcactacc gcagtgcgat atggtgagca agggcgagga 2341 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 2401 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 2461 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 2521 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 2581 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 2641 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 2701 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 2761 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 2821 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 2881 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 2941 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 3001 cgccgggatc actctcggca tggacgagct gtacaagtaa attccgatgg atccacttaa 3061 cgttactgaa atcatcaaac agcttgacga atctggatat aagatcgttg gtgtcgatgt 3121 cagctccgga gttgagacaa atggtgttca ggatctcgat aagatacgtt catttgtcca 3181 agcagcaaag agtgccttct agtgatttaa tagctccatg tcaacaagaa taaaacgcgt 3241 tttcgggttt acctcttcca gatacagctc atctgcaatg cattaatgca ttgactgcaa 3301 cctagtaacg cctttcaggc tccggcgaag agaagaatag cttagcagag ctattttcat 3361 tttcgggaga cgagatcaag cagatcaacg gtcgtcaaga gacctacgag actgaggaat 3421 ccgctcttgg ctccacgcga ctatatattt gtctctaatt gtactttgac atgctcctct 3481 tctttactct gatagcttga ctatgaaaat tccgtcacca gctcctgggt tcgcaaagat 3541 aattgcatgt ttcttccttg aactctcaag cctacaggac acacattcat cgtaggtata 3601 aacctcgaaa tcatttccta ctaagatggt atacaatagt aaccatgcat ggttgcctag 3661 tgaatgctcc gtaacaccca atacgccggc cgaaactttt ttacaactct cctatgagtc 3721 gtttacccag aatgcacagg tacacttgtt tagaggtaat ccttctttct agaagtcctc 3781 gtgtactgtg taagcgccca cttgcgtcgt ggagttacca gtgattgacc aatgttttat 3841 cttctacagt tctgcctgtc taccccattc tagctgtacc tgactacaga gtagtttaat 3901 tgtggttgac cccacagtcg gaggcggagg aatacagcac cgatgtggcc tgtctccatc 3961 cagattggca cgcaattttt acacgcggaa aagatcgaga tagagtacga ctttaaattt 4021 agtccccggc ggcttctatt ttagaatatt tgagatttga ttctcaagca attgatttgg 4081 ttgggtcacc ctcaattgga taatatacct cattgctcgg ctacttcaac tcatcaatca 4141 ccgtcatacc ccgcatataa ccctccattc ccacgatgtc gtccaagtcg caattgactt 4201 acggtgctcg agccagcaag caccccaatc ctctggcaaa gagacttttt gagattgccg 4261 aagcaaagaa gacaaacgtt accgtctctg ctgatgtgac gacaacccga gaactcctgg 4321 acctcgctga ccgtacggaa gctgttggat ccaatacata tgccgtctag caatggacta 4381 atcaactttt gatgatacag gtctcggtcc ctacatcgcc gtcatcaaga cacacatcga 4441 catcctcacc gatttcagcg tcgacactat caatggcctg aatgtgctgg ctcaaaagta 4501 caactttttg atcttcgagg accgcaaatt catcgacatc ggcaataccg tccagaagca 4561 ataccacggc ggtgctctga ggatctccga atgggcccac attatcaact gcagcgttct 4621 ccctggcgag ggcatcgtcg aggctctggc ccagaccgca tctgcgcaag acttccccta 4681 tggtcctgag agaggactgt tggtcctggc agagatgacc cccaaaggat cgctggctac 4741 gggcgagtat accaaggcat cggttgacta cgctcgcaaa tacaagaact tcgttatggg 4801 tttcgtgtcg acgcgggccc tgacggaagt gcagtcggat gtgtcttcag cctcggagga 4861 tgaagatttc gtggtcttca cgacgggtgt gaacctctct tccaaaggag ataagcttgg 4921 acagcaatac cagactcctg catcggctat tggacgcggt gccgacttta tcatcgccgg 4981 tcgaggcatc tacgctgctc ccgacccggt tgaagctgca cagcggtacc agaaagaagg 5041 ctgggaagct tatatggcca gagtatgcgg caagtcatga tttcctcttg gagcaaaagt 5101 gtagtgccag tacgagtgtt gtggaggaag gctgcataca ttgtgcctgt cattaaacga 5161 tgagctcgtc cgtattggcc cctgtaatgc catgttttcc gcccccaatc gtcaaggttt 5221 tccctttgtt agattcctac cagtcatcta gcaagattaa gctaccgcta agacctcagc 5281 gcggccgcaa atttaaataa aatgaagtga agttcctata ctttctagag aataggaact 5341 tctatagtga gtcgaataag ggcgacacaa aatttattct aaatgcataa taaatactga 5401 taacatctta tagtttgtat tatattttgt attatcgttg acatgtataa ttttgatatc 5461 aaaaactgat tttcccttta ttattttcga gatttatttt cttaattctc tttaacaaac 5521 tagaaatatt gtatatacaa aaaatcataa ataatagatg aatagtttaa ttataggtgt 5581 tcatcaatcg aaaaagcaac gtatcttatt taaagtgcgt tgcttttttc tcatttataa 5641 ggttaaataa ttctcatata tcaagcaaag tgacaggcgc ccttaaatat tctgacaaat 5701 gctctttccc taaactcccc ccataaaaaa acccgccgaa gcgggttttt acgttatttg 5761 cggattaacg attactcgtt atcagaaccg cccagggggc ccgagcttaa gactggccgt 5821 cgttttacaa cacagaaaga gtttgtagaa acgcaaaaag gccatccgtc aggggccttc 5881 tgcttagttt gatgcctggc agttccctac tctcgccttc cgcttcctcg ctcactgact 5941 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 6001 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 6061 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 6121 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 6181 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 6241 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 6301 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 6361 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 6421 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 6481 atgtaggcgg tgctacagag ttcttgaagt ggtgggctaa ctacggctac actagaagaa 6541 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 6601 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 6661 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 6721 ctcagtggaa cgacgcgcgc gtaactcacg ttaagggatt ttggtcatga gcttgcgccg 6781 tcccgtcaag tcagcgtaat gctctgcttt taccaatgct taatcagtga ggcacctatc 6841 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 6901 acgatacggg agggcttacc atctggcccc agcgctgcga tgataccgcg agaaccacgc 6961 tcaccggctc cggatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 7021 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 7081 agtagttcgc cagttaatag tttgcgcaac gttgttgcca tcgctacagg catcgtggtg 7141 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 7201 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 7261 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 7321 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 7381 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 7441 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 7501 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 7561 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 7621 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat attcttcctt 7681 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 7741 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac 7801 attatcgcga gcccatttat acctgaatat ggctcataac accccttgtt tgcctggcgg 7861 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc 7921 cgatggtagt gtggggactc cccatgcgag agtagggaac tgccaggcat caaataaaac 7981 gaaaggctca gtcgaaagac tgggcctttc gcccgggcta attatggggt gtcgccctta 8041 ttcgactcta tagtgaagtt cctattctct agaaagtata ggaacttctg aagtggggat 8101 ttaaatgcgg ccgcgctgag ggtttaatcg acg //