LOCUS BBa_K678063 8112 bp DNA linear 15-Nov-2024 DEFINITION pJEJAM15 ACCESSION BBa_K678063 VERSION BBa_K678063.1 FEATURES Location/Qualifiers misc_feature 1..8 /label=Linker 1 promoter 9..2312 /label=PgpdA promoter 1..2312 /label=BBa_K678024 misc_feature 2996..3016 /label=NLS misc_feature 2313..2320 /label=Linker 2 misc_feature 3017..3019 /label=stop misc_feature 2321..2323 /label=start CDS 2321..2995 /label=RFP misc_feature 2313..3019 /label=BBa_K678034 misc_feature 3020..3027 /label=Linker 4 misc_feature 3028..3778 /label=TtrpC terminator terminator 3020..3778 /label=BBa_K678036 misc_feature 3779..3786 /label=Linker 5 misc_feature 3787..5234 /label=pyrG-cassette CDS 3779..5234 /label=BBa_K678040 misc_feature 5235..5242 /label=Linker 6 misc_feature 5243..8112 /label=pFun misc_feature 5235..8112 /label=BBa_K678046 ORIGIN 1 acgtcgctat tcccttgtat ctctacacac aggctcaaat caataagaag aacggttcgt 61 ctttttcgtt tatatcttgc atcgtcccaa agctattggc gggatattct gtttgcagtt 121 ggctgacttg aagtaatctc tgcagatctt tcgacactga aatacgtcga gcctgctccg 181 cttggaagcg gcgaggagcc tcgtcctgtc acaactacca acatggagta cgataagggc 241 cagttccgcc agctcattaa gagccagttc atgggcgttg gcatgatggc cgtcatgcat 301 ctgtacttca agtacaccaa cgctcttctg atccagtcga tcatccgctg aaggcgcttt 361 cgaatctggt taagatccac gtcttcggga agccagcgac tggtgacctc cagcgtccct 421 ttaaggctgc caacagcttt ctcagccagg gccagcccaa gaccgacaag gcctccctcc 481 agaacgccga gaagaactgg aggggtggtg tcaaggagga gtaagctcct tattgaagtc 541 ggaggacgga gcggtgtcaa gaggatattc ttcgactctg tattatagat aagatgatga 601 ggaattggag gtagcatagc ttcatttgga tttgctttcc aggctgagac tctagcttgg 661 agcatagagg gtcctttggc tttcaatatt ctcaagtatc tcgagtttga acttattccc 721 tgtgaacctt ttattcacca atgagcattg gaatgaacat gaatctgagg actgcaatcg 781 ccatgaggtt ttcgaaatac atccggatgt cgaaggcttg gggcacctgc gttggttgaa 841 tttagaacgt ggcactattg atcatccgat agctctgcaa agggcgttgc acaatgcaag 901 tcaaacgttg ctagcagttc caggtggaat gttatgatga gcattgtatt aaatcaggag 961 atatagcatg atctctagtt agctcaccac aaaagtcaga cggcgtaacc aaaagtcaca 1021 caacacaagc tgtaaggatt tcggcacggc tacggaagac ggagaagcca ccttcagtgg 1081 actcgagtac catttaattc tatttgtgtt tgatcgagac ctaatacagc ccctacaacg 1141 accatcaaag tcgtatagct accagtgagg aagtggactc aaatcgactt cagcaacatc 1201 tcctggataa actttaagcc taaactatac agaataagat aggtggagag cttataccga 1261 gctcccaaat ctgtccagat catggttgac cggtgcctgg atcttcctat agaatcatcc 1321 ttattcgttg acctagctga ttctggagtg acccagaggg tcatgacttg agcctaaaat 1381 ccgccgcctc caccatttgt agaaaaatgt gacgaactcg tgagctctgt acagtgaccg 1441 gtgactcttt ctggcatgcg gagagacgga cggacgcaga gagaagggct gagtaataag 1501 ccactggcca gacagctctg gcggctctga ggtgcagtgg atgattatta atccgggacc 1561 ggccgcccct ccgccccgaa gtggaaaggc tggtgtgccc ctcgttgacc aagaatctat 1621 tgcatcatcg gagaatatgg agcttcatcg aatcaccggc agtaagcgaa ggagaatgtg 1681 aagccagggg tgtatagccg tcggcgaaat agcatgccat taacctaggt acagaagtcc 1741 aattgcttcc gatctggtaa aagattcacg agatagtacc ttctccgaag taggtagagc 1801 gagtacccgg cgcgtaagct ccctaattgg cccatccggc atctgtaggg cgtccaaata 1861 tcgtgcctct cctgctttgc ccggtgtatg aaaccggaaa ggccgctcag gagctggcca 1921 gcggcgcaga ccgggaacac aagctggcag tcgacccatc cggtgctctg cactcgacct 1981 gctgaggtcc ctcagtccct ggtaggcagc tttgccccgt ctgtccgccc ggtgtgtcgg 2041 cggggttgac aaggtcgttg cgtcagtcca acatttgttg ccatattttc ctgctctccc 2101 caccagctgc tcttttcttt tctctttctt ttcccatctt cagtatattc atcttcccat 2161 ccaagaacct ttatttcccc taagtaagta ctttgctaca tccatactcc atccttccca 2221 tcccttattc ctttgaacct ttcagttcga gctttcccac ttcatcgcag cttgactaac 2281 agctaccccg cttgagcaga catcactacc gcagtgcgat atggcctcct ccgaggacgt 2341 catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg gccacgagtt 2401 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 2461 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccctc agttccagta 2521 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt 2581 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt 2641 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac 2701 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac 2761 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagatga ggctgaagct 2821 gaaggacggc ggccactacg acgccgaggt caagaccacc tacatggcca agaagcccgt 2881 gcagctgccc ggcgcctaca agaccgacat caagctggac atcacctccc acaacgagga 2941 ctacaccatc gtggaacagt acgagcgcgc cgagggccgc cactccaccg gcgcccccaa 3001 gaaaaagcgc aaggtctaaa ttccgatgga tccacttaac gttactgaaa tcatcaaaca 3061 gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag ttgagacaaa 3121 tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga gtgccttcta 3181 gtgatttaat agctccatgt caacaagaat aaaacgcgtt ttcgggttta cctcttccag 3241 atacagctca tctgcaatgc attaatgcat tgactgcaac ctagtaacgc ctttcaggct 3301 ccggcgaaga gaagaatagc ttagcagagc tattttcatt ttcgggagac gagatcaagc 3361 agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc tccacgcgac 3421 tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg atagcttgac 3481 tatgaaaatt ccgtcaccag ctcctgggtt cgcaaagata attgcatgtt tcttccttga 3541 actctcaagc ctacaggaca cacattcatc gtaggtataa acctcgaaat catttcctac 3601 taagatggta tacaatagta accatgcatg gttgcctagt gaatgctccg taacacccaa 3661 tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga atgcacaggt 3721 acacttgttt agaggtaatc cttctttcta gaagtcctcg tgtactgtgt aagcgcccac 3781 ttgcgtcgtg gagttaccag tgattgacca atgttttatc ttctacagtt ctgcctgtct 3841 accccattct agctgtacct gactacagag tagtttaatt gtggttgacc ccacagtcgg 3901 aggcggagga atacagcacc gatgtggcct gtctccatcc agattggcac gcaattttta 3961 cacgcggaaa agatcgagat agagtacgac tttaaattta gtccccggcg gcttctattt 4021 tagaatattt gagatttgat tctcaagcaa ttgatttggt tgggtcaccc tcaattggat 4081 aatatacctc attgctcggc tacttcaact catcaatcac cgtcataccc cgcatataac 4141 cctccattcc cacgatgtcg tccaagtcgc aattgactta cggtgctcga gccagcaagc 4201 accccaatcc tctggcaaag agactttttg agattgccga agcaaagaag acaaacgtta 4261 ccgtctctgc tgatgtgacg acaacccgag aactcctgga cctcgctgac cgtacggaag 4321 ctgttggatc caatacatat gccgtctagc aatggactaa tcaacttttg atgatacagg 4381 tctcggtccc tacatcgccg tcatcaagac acacatcgac atcctcaccg atttcagcgt 4441 cgacactatc aatggcctga atgtgctggc tcaaaagtac aactttttga tcttcgagga 4501 ccgcaaattc atcgacatcg gcaataccgt ccagaagcaa taccacggcg gtgctctgag 4561 gatctccgaa tgggcccaca ttatcaactg cagcgttctc cctggcgagg gcatcgtcga 4621 ggctctggcc cagaccgcat ctgcgcaaga cttcccctat ggtcctgaga gaggactgtt 4681 ggtcctggca gagatgaccc ccaaaggatc gctggctacg ggcgagtata ccaaggcatc 4741 ggttgactac gctcgcaaat acaagaactt cgttatgggt ttcgtgtcga cgcgggccct 4801 gacggaagtg cagtcggatg tgtcttcagc ctcggaggat gaagatttcg tggtcttcac 4861 gacgggtgtg aacctctctt ccaaaggaga taagcttgga cagcaatacc agactcctgc 4921 atcggctatt ggacgcggtg ccgactttat catcgccggt cgaggcatct acgctgctcc 4981 cgacccggtt gaagctgcac agcggtacca gaaagaaggc tgggaagctt atatggccag 5041 agtatgcggc aagtcatgat ttcctcttgg agcaaaagtg tagtgccagt acgagtgttg 5101 tggaggaagg ctgcatacat tgtgcctgtc attaaacgat gagctcgtcc gtattggccc 5161 ctgtaatgcc atgttttccg cccccaatcg tcaaggtttt ccctttgtta gattcctacc 5221 agtcatctag caagattaag ctaccgctaa gacctcagcg cggccgcaaa tttaaataaa 5281 atgaagtgaa gttcctatac tttctagaga ataggaactt ctatagtgag tcgaataagg 5341 gcgacacaaa atttattcta aatgcataat aaatactgat aacatcttat agtttgtatt 5401 atattttgta ttatcgttga catgtataat tttgatatca aaaactgatt ttccctttat 5461 tattttcgag atttattttc ttaattctct ttaacaaact agaaatattg tatatacaaa 5521 aaatcataaa taatagatga atagtttaat tataggtgtt catcaatcga aaaagcaacg 5581 tatcttattt aaagtgcgtt gcttttttct catttataag gttaaataat tctcatatat 5641 caagcaaagt gacaggcgcc cttaaatatt ctgacaaatg ctctttccct aaactccccc 5701 cataaaaaaa cccgccgaag cgggttttta cgttatttgc ggattaacga ttactcgtta 5761 tcagaaccgc ccagggggcc cgagcttaag actggccgtc gttttacaac acagaaagag 5821 tttgtagaaa cgcaaaaagg ccatccgtca ggggccttct gcttagtttg atgcctggca 5881 gttccctact ctcgccttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 5941 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 6001 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 6061 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 6121 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 6181 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 6241 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 6301 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 6361 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 6421 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 6481 tcttgaagtg gtgggctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 6541 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 6601 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 6661 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gacgcgcgcg 6721 taactcacgt taagggattt tggtcatgag cttgcgccgt cccgtcaagt cagcgtaatg 6781 ctctgctttt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 6841 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 6901 tctggcccca gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca 6961 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 7021 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 7081 ttgcgcaacg ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 7141 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 7201 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 7261 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 7321 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 7381 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 7441 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 7501 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 7561 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 7621 agggcgacac ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt 7681 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 7741 ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata 7801 cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc 7861 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc 7921 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact 7981 gggcctttcg cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc 8041 ctattctcta gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg 8101 gtttaatcga cg //