LOCUS BBa_K678064 8362 bp DNA linear 29-Nov-2024 DEFINITION pJEJAM16 ACCESSION BBa_K678064 VERSION BBa_K678064.1 FEATURES Location/Qualifiers misc_feature 1..8 /label=Linker 1 promoter 9..2312 /label=PgpdA promoter 1..2312 /label=BBa_K678024 misc_feature 2329..2508 /label=MTS misc_feature 2321..2328 /label=Linker 2 misc_feature 2321..2508 /label=BBa_K678025 misc_feature 2517..2525 /label=Linker 3 CDS 2526..3245 /label=GFP misc_feature 2517..3245 /label=BBa_K678029 misc_feature 3254..3261 /label=Linker 4 misc_feature 3262..4012 /label=TtrpC terminator terminator 3254..4012 /label=BBa_K678036 misc_feature 4021..4028 /label=Linker 5 misc_feature 4029..5476 /label=pyrG-cassette CDS 4021..5476 /label=BBa_K678040 misc_feature 5485..5492 /label=Linker 6 misc_feature 5493..8362 /label=pFun misc_feature 5485..8362 /label=BBa_K678046 ORIGIN 1 acgtcgctat tcccttgtat ctctacacac aggctcaaat caataagaag aacggttcgt 61 ctttttcgtt tatatcttgc atcgtcccaa agctattggc gggatattct gtttgcagtt 121 ggctgacttg aagtaatctc tgcagatctt tcgacactga aatacgtcga gcctgctccg 181 cttggaagcg gcgaggagcc tcgtcctgtc acaactacca acatggagta cgataagggc 241 cagttccgcc agctcattaa gagccagttc atgggcgttg gcatgatggc cgtcatgcat 301 ctgtacttca agtacaccaa cgctcttctg atccagtcga tcatccgctg aaggcgcttt 361 cgaatctggt taagatccac gtcttcggga agccagcgac tggtgacctc cagcgtccct 421 ttaaggctgc caacagcttt ctcagccagg gccagcccaa gaccgacaag gcctccctcc 481 agaacgccga gaagaactgg aggggtggtg tcaaggagga gtaagctcct tattgaagtc 541 ggaggacgga gcggtgtcaa gaggatattc ttcgactctg tattatagat aagatgatga 601 ggaattggag gtagcatagc ttcatttgga tttgctttcc aggctgagac tctagcttgg 661 agcatagagg gtcctttggc tttcaatatt ctcaagtatc tcgagtttga acttattccc 721 tgtgaacctt ttattcacca atgagcattg gaatgaacat gaatctgagg actgcaatcg 781 ccatgaggtt ttcgaaatac atccggatgt cgaaggcttg gggcacctgc gttggttgaa 841 tttagaacgt ggcactattg atcatccgat agctctgcaa agggcgttgc acaatgcaag 901 tcaaacgttg ctagcagttc caggtggaat gttatgatga gcattgtatt aaatcaggag 961 atatagcatg atctctagtt agctcaccac aaaagtcaga cggcgtaacc aaaagtcaca 1021 caacacaagc tgtaaggatt tcggcacggc tacggaagac ggagaagcca ccttcagtgg 1081 actcgagtac catttaattc tatttgtgtt tgatcgagac ctaatacagc ccctacaacg 1141 accatcaaag tcgtatagct accagtgagg aagtggactc aaatcgactt cagcaacatc 1201 tcctggataa actttaagcc taaactatac agaataagat aggtggagag cttataccga 1261 gctcccaaat ctgtccagat catggttgac cggtgcctgg atcttcctat agaatcatcc 1321 ttattcgttg acctagctga ttctggagtg acccagaggg tcatgacttg agcctaaaat 1381 ccgccgcctc caccatttgt agaaaaatgt gacgaactcg tgagctctgt acagtgaccg 1441 gtgactcttt ctggcatgcg gagagacgga cggacgcaga gagaagggct gagtaataag 1501 ccactggcca gacagctctg gcggctctga ggtgcagtgg atgattatta atccgggacc 1561 ggccgcccct ccgccccgaa gtggaaaggc tggtgtgccc ctcgttgacc aagaatctat 1621 tgcatcatcg gagaatatgg agcttcatcg aatcaccggc agtaagcgaa ggagaatgtg 1681 aagccagggg tgtatagccg tcggcgaaat agcatgccat taacctaggt acagaagtcc 1741 aattgcttcc gatctggtaa aagattcacg agatagtacc ttctccgaag taggtagagc 1801 gagtacccgg cgcgtaagct ccctaattgg cccatccggc atctgtaggg cgtccaaata 1861 tcgtgcctct cctgctttgc ccggtgtatg aaaccggaaa ggccgctcag gagctggcca 1921 gcggcgcaga ccgggaacac aagctggcag tcgacccatc cggtgctctg cactcgacct 1981 gctgaggtcc ctcagtccct ggtaggcagc tttgccccgt ctgtccgccc ggtgtgtcgg 2041 cggggttgac aaggtcgttg cgtcagtcca acatttgttg ccatattttc ctgctctccc 2101 caccagctgc tcttttcttt tctctttctt ttcccatctt cagtatattc atcttcccat 2161 ccaagaacct ttatttcccc taagtaagta ctttgctaca tccatactcc atccttccca 2221 tcccttattc ctttgaacct ttcagttcga gctttcccac ttcatcgcag cttgactaac 2281 agctaccccg cttgagcaga catcactacc gctactagag agtgcgatat gtttacagcg 2341 gcagctcgga gccgtttctc ttctactctt gctcgccccc gtctcgctcc gacaaattcc 2401 cttctcgcta gatcttcagt ggtaggatac ttcttttctt tttaccctgc agatatcgga 2461 gtctggttaa cagattatca ggcttcaaca atggctccgc ggcgcaagta ctagagagcg 2521 ctggtatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 2581 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 2641 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 2701 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca 2761 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 2821 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 2881 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 2941 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga 3001 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc 3061 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 3121 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 3181 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 3241 agtaatacta gagattccga tggatccact taacgttact gaaatcatca aacagcttga 3301 cgaatctgga tataagatcg ttggtgtcga tgtcagctcc ggagttgaga caaatggtgt 3361 tcaggatctc gataagatac gttcatttgt ccaagcagca aagagtgcct tctagtgatt 3421 taatagctcc atgtcaacaa gaataaaacg cgttttcggg tttacctctt ccagatacag 3481 ctcatctgca atgcattaat gcattgactg caacctagta acgcctttca ggctccggcg 3541 aagagaagaa tagcttagca gagctatttt cattttcggg agacgagatc aagcagatca 3601 acggtcgtca agagacctac gagactgagg aatccgctct tggctccacg cgactatata 3661 tttgtctcta attgtacttt gacatgctcc tcttctttac tctgatagct tgactatgaa 3721 aattccgtca ccagctcctg ggttcgcaaa gataattgca tgtttcttcc ttgaactctc 3781 aagcctacag gacacacatt catcgtaggt ataaacctcg aaatcatttc ctactaagat 3841 ggtatacaat agtaaccatg catggttgcc tagtgaatgc tccgtaacac ccaatacgcc 3901 ggccgaaact tttttacaac tctcctatga gtcgtttacc cagaatgcac aggtacactt 3961 gtttagaggt aatccttctt tctagaagtc ctcgtgtact gtgtaagcgc cctactagag 4021 acttgcgtcg tggagttacc agtgattgac caatgtttta tcttctacag ttctgcctgt 4081 ctaccccatt ctagctgtac ctgactacag agtagtttaa ttgtggttga ccccacagtc 4141 ggaggcggag gaatacagca ccgatgtggc ctgtctccat ccagattggc acgcaatttt 4201 tacacgcgga aaagatcgag atagagtacg actttaaatt tagtccccgg cggcttctat 4261 tttagaatat ttgagatttg attctcaagc aattgatttg gttgggtcac cctcaattgg 4321 ataatatacc tcattgctcg gctacttcaa ctcatcaatc accgtcatac cccgcatata 4381 accctccatt cccacgatgt cgtccaagtc gcaattgact tacggtgctc gagccagcaa 4441 gcaccccaat cctctggcaa agagactttt tgagattgcc gaagcaaaga agacaaacgt 4501 taccgtctct gctgatgtga cgacaacccg agaactcctg gacctcgctg accgtacgga 4561 agctgttgga tccaatacat atgccgtcta gcaatggact aatcaacttt tgatgataca 4621 ggtctcggtc cctacatcgc cgtcatcaag acacacatcg acatcctcac cgatttcagc 4681 gtcgacacta tcaatggcct gaatgtgctg gctcaaaagt acaacttttt gatcttcgag 4741 gaccgcaaat tcatcgacat cggcaatacc gtccagaagc aataccacgg cggtgctctg 4801 aggatctccg aatgggccca cattatcaac tgcagcgttc tccctggcga gggcatcgtc 4861 gaggctctgg cccagaccgc atctgcgcaa gacttcccct atggtcctga gagaggactg 4921 ttggtcctgg cagagatgac ccccaaagga tcgctggcta cgggcgagta taccaaggca 4981 tcggttgact acgctcgcaa atacaagaac ttcgttatgg gtttcgtgtc gacgcgggcc 5041 ctgacggaag tgcagtcgga tgtgtcttca gcctcggagg atgaagattt cgtggtcttc 5101 acgacgggtg tgaacctctc ttccaaagga gataagcttg gacagcaata ccagactcct 5161 gcatcggcta ttggacgcgg tgccgacttt atcatcgccg gtcgaggcat ctacgctgct 5221 cccgacccgg ttgaagctgc acagcggtac cagaaagaag gctgggaagc ttatatggcc 5281 agagtatgcg gcaagtcatg atttcctctt ggagcaaaag tgtagtgcca gtacgagtgt 5341 tgtggaggaa ggctgcatac attgtgcctg tcattaaacg atgagctcgt ccgtattggc 5401 ccctgtaatg ccatgttttc cgcccccaat cgtcaaggtt ttccctttgt tagattccta 5461 ccagtcatct agcaagtact agagattaag ctaccgctaa gacctcagcg cggccgcaaa 5521 tttaaataaa atgaagtgaa gttcctatac tttctagaga ataggaactt ctatagtgag 5581 tcgaataagg gcgacacaaa atttattcta aatgcataat aaatactgat aacatcttat 5641 agtttgtatt atattttgta ttatcgttga catgtataat tttgatatca aaaactgatt 5701 ttccctttat tattttcgag atttattttc ttaattctct ttaacaaact agaaatattg 5761 tatatacaaa aaatcataaa taatagatga atagtttaat tataggtgtt catcaatcga 5821 aaaagcaacg tatcttattt aaagtgcgtt gcttttttct catttataag gttaaataat 5881 tctcatatat caagcaaagt gacaggcgcc cttaaatatt ctgacaaatg ctctttccct 5941 aaactccccc cataaaaaaa cccgccgaag cgggttttta cgttatttgc ggattaacga 6001 ttactcgtta tcagaaccgc ccagggggcc cgagcttaag actggccgtc gttttacaac 6061 acagaaagag tttgtagaaa cgcaaaaagg ccatccgtca ggggccttct gcttagtttg 6121 atgcctggca gttccctact ctcgccttcc gcttcctcgc tcactgactc gctgcgctcg 6181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 6241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 6301 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 6361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 6421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 6481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 6541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 6601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 6661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 6721 gctacagagt tcttgaagtg gtgggctaac tacggctaca ctagaagaac agtatttggt 6781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 6841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 6901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 6961 gacgcgcgcg taactcacgt taagggattt tggtcatgag cttgcgccgt cccgtcaagt 7021 cagcgtaatg ctctgctttt accaatgctt aatcagtgag gcacctatct cagcgatctg 7081 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 7141 gggcttacca tctggcccca gcgctgcgat gataccgcga gaaccacgct caccggctcc 7201 ggatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 7261 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 7321 agttaatagt ttgcgcaacg ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc 7381 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 7441 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 7501 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 7561 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 7621 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 7681 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 7741 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 7801 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 7861 aaagggaata agggcgacac ggaaatgttg aatactcata ttcttccttt ttcaatatta 7921 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 7981 aaataaacaa ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag 8041 cccatttata cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg 8101 tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg 8161 tggggactcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag 8221 tcgaaagact gggcctttcg cccgggctaa ttatggggtg tcgcccttat tcgactctat 8281 agtgaagttc ctattctcta gaaagtatag gaacttctga agtggggatt taaatgcggc 8341 cgcgctgagg gtttaatcga cg //