LOCUS BBa_K678065 8269 bp DNA linear 22-Dec-2024 DEFINITION pJEJAM17 ACCESSION BBa_K678065 VERSION BBa_K678065.1 FEATURES Location/Qualifiers misc_feature 1..8 /label=Linker 1 promoter 9..2312 /label=PgpdA promoter 1..2312 /label=BBa_K678024 misc_feature 2329..2508 /label=MTS misc_feature 2321..2328 /label=Linker 2 misc_feature 2321..2508 /label=BBa_K678025 misc_feature 2517..2525 /label=Linker 3 CDS 2526..3245 /label=GFP misc_feature 2517..3245 /label=BBa_K678029 misc_feature 3254..3261 /label=Linker 4 misc_feature 3262..4012 /label=TtrpC terminator terminator 3254..4012 /label=BBa_K678036 misc_feature 4021..4028 /label=Linker 5 misc_feature 4029..5383 /label=hygR cassette CDS 4021..5383 /label=BBa_K678042 misc_feature 5392..5399 /label=Linker 6 misc_feature 5400..8269 /label=pFun misc_feature 5392..8269 /label=BBa_K678046 ORIGIN 1 acgtcgctat tcccttgtat ctctacacac aggctcaaat caataagaag aacggttcgt 61 ctttttcgtt tatatcttgc atcgtcccaa agctattggc gggatattct gtttgcagtt 121 ggctgacttg aagtaatctc tgcagatctt tcgacactga aatacgtcga gcctgctccg 181 cttggaagcg gcgaggagcc tcgtcctgtc acaactacca acatggagta cgataagggc 241 cagttccgcc agctcattaa gagccagttc atgggcgttg gcatgatggc cgtcatgcat 301 ctgtacttca agtacaccaa cgctcttctg atccagtcga tcatccgctg aaggcgcttt 361 cgaatctggt taagatccac gtcttcggga agccagcgac tggtgacctc cagcgtccct 421 ttaaggctgc caacagcttt ctcagccagg gccagcccaa gaccgacaag gcctccctcc 481 agaacgccga gaagaactgg aggggtggtg tcaaggagga gtaagctcct tattgaagtc 541 ggaggacgga gcggtgtcaa gaggatattc ttcgactctg tattatagat aagatgatga 601 ggaattggag gtagcatagc ttcatttgga tttgctttcc aggctgagac tctagcttgg 661 agcatagagg gtcctttggc tttcaatatt ctcaagtatc tcgagtttga acttattccc 721 tgtgaacctt ttattcacca atgagcattg gaatgaacat gaatctgagg actgcaatcg 781 ccatgaggtt ttcgaaatac atccggatgt cgaaggcttg gggcacctgc gttggttgaa 841 tttagaacgt ggcactattg atcatccgat agctctgcaa agggcgttgc acaatgcaag 901 tcaaacgttg ctagcagttc caggtggaat gttatgatga gcattgtatt aaatcaggag 961 atatagcatg atctctagtt agctcaccac aaaagtcaga cggcgtaacc aaaagtcaca 1021 caacacaagc tgtaaggatt tcggcacggc tacggaagac ggagaagcca ccttcagtgg 1081 actcgagtac catttaattc tatttgtgtt tgatcgagac ctaatacagc ccctacaacg 1141 accatcaaag tcgtatagct accagtgagg aagtggactc aaatcgactt cagcaacatc 1201 tcctggataa actttaagcc taaactatac agaataagat aggtggagag cttataccga 1261 gctcccaaat ctgtccagat catggttgac cggtgcctgg atcttcctat agaatcatcc 1321 ttattcgttg acctagctga ttctggagtg acccagaggg tcatgacttg agcctaaaat 1381 ccgccgcctc caccatttgt agaaaaatgt gacgaactcg tgagctctgt acagtgaccg 1441 gtgactcttt ctggcatgcg gagagacgga cggacgcaga gagaagggct gagtaataag 1501 ccactggcca gacagctctg gcggctctga ggtgcagtgg atgattatta atccgggacc 1561 ggccgcccct ccgccccgaa gtggaaaggc tggtgtgccc ctcgttgacc aagaatctat 1621 tgcatcatcg gagaatatgg agcttcatcg aatcaccggc agtaagcgaa ggagaatgtg 1681 aagccagggg tgtatagccg tcggcgaaat agcatgccat taacctaggt acagaagtcc 1741 aattgcttcc gatctggtaa aagattcacg agatagtacc ttctccgaag taggtagagc 1801 gagtacccgg cgcgtaagct ccctaattgg cccatccggc atctgtaggg cgtccaaata 1861 tcgtgcctct cctgctttgc ccggtgtatg aaaccggaaa ggccgctcag gagctggcca 1921 gcggcgcaga ccgggaacac aagctggcag tcgacccatc cggtgctctg cactcgacct 1981 gctgaggtcc ctcagtccct ggtaggcagc tttgccccgt ctgtccgccc ggtgtgtcgg 2041 cggggttgac aaggtcgttg cgtcagtcca acatttgttg ccatattttc ctgctctccc 2101 caccagctgc tcttttcttt tctctttctt ttcccatctt cagtatattc atcttcccat 2161 ccaagaacct ttatttcccc taagtaagta ctttgctaca tccatactcc atccttccca 2221 tcccttattc ctttgaacct ttcagttcga gctttcccac ttcatcgcag cttgactaac 2281 agctaccccg cttgagcaga catcactacc gctactagag agtgcgatat gtttacagcg 2341 gcagctcgga gccgtttctc ttctactctt gctcgccccc gtctcgctcc gacaaattcc 2401 cttctcgcta gatcttcagt ggtaggatac ttcttttctt tttaccctgc agatatcgga 2461 gtctggttaa cagattatca ggcttcaaca atggctccgc ggcgcaagta ctagagagcg 2521 ctggtatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 2581 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 2641 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 2701 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca 2761 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 2821 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 2881 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 2941 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga 3001 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc 3061 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 3121 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 3181 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 3241 agtaatacta gagattccga tggatccact taacgttact gaaatcatca aacagcttga 3301 cgaatctgga tataagatcg ttggtgtcga tgtcagctcc ggagttgaga caaatggtgt 3361 tcaggatctc gataagatac gttcatttgt ccaagcagca aagagtgcct tctagtgatt 3421 taatagctcc atgtcaacaa gaataaaacg cgttttcggg tttacctctt ccagatacag 3481 ctcatctgca atgcattaat gcattgactg caacctagta acgcctttca ggctccggcg 3541 aagagaagaa tagcttagca gagctatttt cattttcggg agacgagatc aagcagatca 3601 acggtcgtca agagacctac gagactgagg aatccgctct tggctccacg cgactatata 3661 tttgtctcta attgtacttt gacatgctcc tcttctttac tctgatagct tgactatgaa 3721 aattccgtca ccagctcctg ggttcgcaaa gataattgca tgtttcttcc ttgaactctc 3781 aagcctacag gacacacatt catcgtaggt ataaacctcg aaatcatttc ctactaagat 3841 ggtatacaat agtaaccatg catggttgcc tagtgaatgc tccgtaacac ccaatacgcc 3901 ggccgaaact tttttacaac tctcctatga gtcgtttacc cagaatgcac aggtacactt 3961 gtttagaggt aatccttctt tctagaagtc ctcgtgtact gtgtaagcgc cctactagag 4021 acttgcgtgc tagtggaggt caacacatca atgctatttt ggtttagtcg tccaggcgga 4081 tcacaaaatt tgtgtcgttt gacaagatgg ttcatttagg caactggtca gatcagccca 4141 cttgtaagca gtagcggcgg cgctcgaagt gtgactctta ttagcagaca ggaacgagga 4201 cattattatc atctgctgct tggtgcacga taacttgtgc gtttgtcaag caaggtaagt 4261 gaacgacccg gtcatacctt cttaagttcg cccttcctcc ctttatttca gattcaatct 4321 gacttaccta ttctacccaa gcatccaaat gcctgaactc accgcgacgt ctgtcgagaa 4381 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga 4441 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg 4501 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc 4561 gattccggaa gtgcttgaca ttggggagtt cagcgagagc ctgacctatt gcatctcccg 4621 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgga 4681 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt 4741 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc 4801 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc 4861 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca 4921 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc 4981 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt 5041 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca 5101 tccggagctt gcaggatcgc cgcgcctccg ggcgtatatg ctccgcattg gtcttgacca 5161 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg 5221 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag 5281 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc 5341 cagcactcgt ccgagggcaa aggaatagag tagatgccga ccgtactaga gattaagcta 5401 ccgctaagac ctcagcgcgg ccgcaaattt aaataaaatg aagtgaagtt cctatacttt 5461 ctagagaata ggaacttcta tagtgagtcg aataagggcg acacaaaatt tattctaaat 5521 gcataataaa tactgataac atcttatagt ttgtattata ttttgtatta tcgttgacat 5581 gtataatttt gatatcaaaa actgattttc cctttattat tttcgagatt tattttctta 5641 attctcttta acaaactaga aatattgtat atacaaaaaa tcataaataa tagatgaata 5701 gtttaattat aggtgttcat caatcgaaaa agcaacgtat cttatttaaa gtgcgttgct 5761 tttttctcat ttataaggtt aaataattct catatatcaa gcaaagtgac aggcgccctt 5821 aaatattctg acaaatgctc tttccctaaa ctccccccat aaaaaaaccc gccgaagcgg 5881 gtttttacgt tatttgcgga ttaacgatta ctcgttatca gaaccgccca gggggcccga 5941 gcttaagact ggccgtcgtt ttacaacaca gaaagagttt gtagaaacgc aaaaaggcca 6001 tccgtcaggg gccttctgct tagtttgatg cctggcagtt ccctactctc gccttccgct 6061 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 6121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 6181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 6241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 6301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 6361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 6421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 6481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 6541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 6601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg ggctaactac 6661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 6721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 6781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 6841 tctacggggt ctgacgctca gtggaacgac gcgcgcgtaa ctcacgttaa gggattttgg 6901 tcatgagctt gcgccgtccc gtcaagtcag cgtaatgctc tgcttttacc aatgcttaat 6961 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 7021 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagcg ctgcgatgat 7081 accgcgagaa ccacgctcac cggctccgga tttatcagca ataaaccagc cagccggaag 7141 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 7201 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccatcgc 7261 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 7321 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 7381 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 7441 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 7501 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 7561 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 7621 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 7681 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 7741 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 7801 actcatattc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 7861 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggtcagtg ttacaaccaa 7921 ttaaccaatt ctgaacatta tcgcgagccc atttatacct gaatatggct cataacaccc 7981 cttgtttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag 8041 tgaaacgccg tagcgccgat ggtagtgtgg ggactcccca tgcgagagta gggaactgcc 8101 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgccc gggctaatta 8161 tggggtgtcg cccttattcg actctatagt gaagttccta ttctctagaa agtataggaa 8221 cttctgaagt ggggatttaa atgcggccgc gctgagggtt taatcgacg //