PgpdA, promoter for Aspergillus nidulans
From the plasmid p71 from the plasmid collection of the Center for Microbial Biotechnology at the Technical University of Denmark
Strong constitutive Aspergillus nidulans promoter
Compatible with the Plug'n'Play assembly standard
Mitochondrial targeting sequence for Aspergillus nidulans
Aspergillus nidulans genomic DNA (AN6279)
Mitochondrial targeting sequence (MTS) for Aspergillus nidulans. A MTS is usually required for the entry of proteins and intermediary metabolites into mitochondria. The MTS is a DNA fragment encoding the N-terminal 60 amino acids of the AcuJ.
Compatible with the Plug'n'Play assembly standard. This MTS should be placed at the N-terminal of the gene of interest.
trpC terminator for Aspergillus nidulans
From the plasmid p71 from the Center for Microbial Biotechnology at the Technical University of Denmark plasmid collection.
TtrpC is a terminator of tryptophan synthase of Aspergillus nidulans.
hygromycin cassette for Aspergillus nidulans
Plasmid for Plug'n'Play. Contains an Ampicillin resistance marker.
Compatible with the Plug'n'Play assembly standard.
Design considerations This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
Conversion of the iGEM parts registry to SBOL2.1