LOCUS BBa_K678066 7531 bp DNA linear 15-Nov-2024 DEFINITION pJEJAM18 ACCESSION BBa_K678066 VERSION BBa_K678066.1 FEATURES Location/Qualifiers misc_feature 1..8 /label=Linker 1 promoter 9..548 /label=DMKP-P6 promoter 1..548 /label=BBa_K678000 misc_feature 557..564 /label=Linker 2 CDS 565..1281 /label=GFP misc_feature 557..1281 /label=BBa_K678028 misc_feature 1290..1298 /label=Linker 3 CDS 1299..1976 /label=RFP misc_feature 1290..1976 /label=BBa_K678032 misc_feature 1985..1992 /label=Linker 4 misc_feature 1993..2974 /label=T2-motni terminator terminator 1985..2974 /label=BBa_K678038 misc_feature 2983..2990 /label=Linker 5 misc_feature 2991..4645 /label=bleR cassette CDS 2983..4645 /label=BBa_K678044 misc_feature 4654..4661 /label=Linker 6 misc_feature 4662..7531 /label=pFun misc_feature 4654..7531 /label=BBa_K678046 ORIGIN 1 acgtcgctat ctcactccac tagaattcct gtcttctgat tcgacggacg cgtatactgc 61 gctaacaaat ccataccaag tagatttggg cgagctgggt gacatttgga atatgggaaa 121 cctcgagtta cctcgagatt aatatgacca atgtaccata tagttagcgt cgctgtgttt 181 ggatttacta gcgttatgat tgttgatgcg aggtctcaca acctgcgatt taccggcgag 241 tgccaatcaa gttgatagtt tacgtgactg aagacttggg tggctgctga aacaatgcat 301 atcaaaacac cctcgtatat atagcagaca attagcgtcg tagactatat tcaaggacat 361 attccatttc tcgattctca gctcatgtat cagcctaaag acggcaacag tagatgcaaa 421 tccgatagat gagtcatcaa ggctagatta gggcaacgct attcccaatt cggctagcgt 481 atattgcacc cacaagcgga gaccagattt tcagagccac catcgaaccc gccattctcg 541 actcatttta ctagagagtg cgatatggtg agcaagggcg aggagctgtt caccggggtg 601 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 661 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 721 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc 781 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc 841 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 901 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 961 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat 1021 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc 1081 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 1141 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc 1201 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 1261 ggcatggacg agctgtacaa gtactagaga gcgctggtat ggcctcctcc gaggacgtca 1321 tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg 1381 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 1441 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtcccctcag ttccagtacg 1501 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 1561 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 1621 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 1681 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg 1741 agcggatgta ccccgaggac ggcgccctga agggcgagat caagatgagg ctgaagctga 1801 aggacggcgg ccactacgac gccgaggtca agaccaccta catggccaag aagcccgtgc 1861 agctgcccgg cgcctacaag accgacatca agctggacat cacctcccac aacgaggact 1921 acaccatcgt ggaacagtac gagcgcgccg agggccgcca ctccaccggc gcctaatact 1981 agagattccg atgcggtttt caaggataac agatggcaaa attgggatat cgtcccacgg 2041 gctggtgaaa accagcatcg tgaggattaa tggtggacaa ttctctttat ccctctaccc 2101 ggtgtaatct agagtgtcca taccagttgt caataagttt ccaacaagcc agtgccgttg 2161 tagttgagta tttgcagtat agtcaaccat agtcgtagcc agtaatgttt acgtacaata 2221 tctacagaaa aatgaaataa tttccacctc gtaaattagc acaccgtgac gaaatcccaa 2281 gaacgtggca acctagacag gcactaaaac ccgtactaca ttacaattca accccgctcc 2341 aacaacttct tctccctctt ttctccggtg ggggagggaa agagaggagg gaggggggaa 2401 agttcgagat ctggcaattc gaccctcgga ttggtttccc ggttccgaat acggccggct 2461 ttcccattcc actttgcttt ccttgcttgc tgcgtgctgc gtgctgctgt gttgcatgca 2521 gctagcctta atgagactac ctatctttaa tgtagagtag tatgagcagg gaaagccttt 2581 atttcaactt tctttcttcc ttgattttgg ttgataatat agatctgcac catgatatag 2641 tattatagaa tggatagatg aatgaatgga tgaatggtat gtatacgtaa gtacctcgaa 2701 agttagttaa gtaaaaagtg aggggtaagt tgagagccag ccaaggctgg ggggctaaaa 2761 tatgatgtga tcggatcacg agtatatgtc gtagttgttg tgtgtttttg tgtaggattg 2821 gattggattg gattgggttg agttagtggc attgccgaca tagccattca atgagtcgag 2881 gaatgaactg gtttcccgcg ccagtaaata acttacttta gctgtggtgg tagtaagtaa 2941 gatataaccg gttttaaagt tatccggggt catgtactag agacttgcgt taagctccct 3001 aattggccca tccggcatct gtagggcgtc caaatatcgt gcctctcctg ctttgcccgg 3061 tgtatgaaac cggaaaggcc gctcaggagc tggccagcgg cgcagaccgg gaacacaagc 3121 tggcagtcga cccatccggt gctctgcact cgacctgctg aggtccctca gtccctggta 3181 ggcagctttg ccccgtctgt ccgcccggtg tgtcggcggg gttgacaagg tcgttgcgtc 3241 agtccaacat ttgttgccat attttcctgc tctccccacc agctgctctt ttcttttctc 3301 tttcttttcc catcttcagt atattcatct tcccatccaa gaacctttat ttcccctaag 3361 taagtacttt gctacatcca tactccatcc ttcccatccc ttattccttt gaacctttca 3421 gttcgagctt tcccacttca tcgcagcttg actaacagct accccgcttg agcagacatc 3481 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg 3541 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc 3601 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg 3661 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg 3721 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag 3781 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg 3841 gccgaggagc aggactgacc gacgccgacc aacaccgccg gtccgacggc ggcccacggg 3901 tcccaggagc ttgagatcca cttaacgtta ctgaaatcat caaacagctt gacgaatctg 3961 gatataagat cgttggtgtc gatgtcagct ccggagttga gacaaatggt gttcaggatc 4021 tcgataagat acgttcattt gtccaagcag caaagagtgc cttctagtga tttaatagct 4081 ccatgtcaac aagaataaaa cgcgttttcg ggtttacctc ttccagatac agctcatctg 4141 caatgcatta atgcattgac tgcaacctag taacgccttt caggctccgg cgaagagaag 4201 aatagcttag cagagctatt ttcattttcg ggagacgaga tcaagcagat caacggtcgt 4261 caagagacct acgagactga ggaatccgct cttggctcca cgcgactata tatttgtctc 4321 taattgtact ttgacatgct cctcttcttt actctgatag cttgactatg aaaattccgt 4381 caccagctcc tgggttcgca aagataattg catgtttctt ccttgaactc tcaagcctac 4441 aggacacaca ttcatcgtag gtataaacct cgaaatcatt tcctactaag atggtataca 4501 atagtaacca tgcatggttg cctagtgaat gctccgtaac acccaatacg ccggccgaaa 4561 cttttttaca actctcctat gagtcgttta cccagaatgc acaggtacac ttgtttagag 4621 gtaatccttc tttctagagc ttggctacta gagattaagc taccgctaag acctcagcgc 4681 ggccgcaaat ttaaataaaa tgaagtgaag ttcctatact ttctagagaa taggaacttc 4741 tatagtgagt cgaataaggg cgacacaaaa tttattctaa atgcataata aatactgata 4801 acatcttata gtttgtatta tattttgtat tatcgttgac atgtataatt ttgatatcaa 4861 aaactgattt tccctttatt attttcgaga tttattttct taattctctt taacaaacta 4921 gaaatattgt atatacaaaa aatcataaat aatagatgaa tagtttaatt ataggtgttc 4981 atcaatcgaa aaagcaacgt atcttattta aagtgcgttg cttttttctc atttataagg 5041 ttaaataatt ctcatatatc aagcaaagtg acaggcgccc ttaaatattc tgacaaatgc 5101 tctttcccta aactcccccc ataaaaaaac ccgccgaagc gggtttttac gttatttgcg 5161 gattaacgat tactcgttat cagaaccgcc cagggggccc gagcttaaga ctggccgtcg 5221 ttttacaaca cagaaagagt ttgtagaaac gcaaaaaggc catccgtcag gggccttctg 5281 cttagtttga tgcctggcag ttccctactc tcgccttccg cttcctcgct cactgactcg 5341 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 5401 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 5461 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 5521 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 5581 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 5641 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 5701 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 5761 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 5821 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 5881 gtaggcggtg ctacagagtt cttgaagtgg tgggctaact acggctacac tagaagaaca 5941 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 6001 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 6061 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 6121 cagtggaacg acgcgcgcgt aactcacgtt aagggatttt ggtcatgagc ttgcgccgtc 6181 ccgtcaagtc agcgtaatgc tctgctttta ccaatgctta atcagtgagg cacctatctc 6241 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 6301 gatacgggag ggcttaccat ctggccccag cgctgcgatg ataccgcgag aaccacgctc 6361 accggctccg gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 6421 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 6481 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatc gctacaggca tcgtggtgtc 6541 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 6601 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 6661 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 6721 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 6781 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 6841 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 6901 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 6961 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 7021 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatat tcttcctttt 7081 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 7141 tatttagaaa aataaacaaa taggggtcag tgttacaacc aattaaccaa ttctgaacat 7201 tatcgcgagc ccatttatac ctgaatatgg ctcataacac cccttgtttg cctggcggca 7261 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg 7321 atggtagtgt ggggactccc catgcgagag tagggaactg ccaggcatca aataaaacga 7381 aaggctcagt cgaaagactg ggcctttcgc ccgggctaat tatggggtgt cgcccttatt 7441 cgactctata gtgaagttcc tattctctag aaagtatagg aacttctgaa gtggggattt 7501 aaatgcggcc gcgctgaggg tttaatcgac g //