LOCUS BBa_K678068 8230 bp DNA linear 15-Nov-2024 DEFINITION pJEJAM20 ACCESSION BBa_K678068 VERSION BBa_K678068.1 FEATURES Location/Qualifiers misc_feature 844..851 /label=Linker 2 misc_feature 1..8 /label=Linker 1 promoter 9..843 /label=PalcA promoter 1..843 /label=BBa_K678001 misc_feature 852..859 /label=Linker 2 CDS 860..3007 /label=yA CDS 852..3007 /label=BBa_K678035 misc_feature 3016..3024 /label=Linker 3 CDS 3025..3702 /label=RFP misc_feature 3016..3702 /label=BBa_K678032 misc_feature 3711..3718 /label=Linker 4 misc_feature 3719..4469 /label=TtrpC terminator terminator 3711..4469 /label=BBa_K678036 misc_feature 4478..4485 /label=Linker 5 misc_feature 4486..5344 /label=ampR cassette misc_feature 4478..5344 /label=BBa_K678047 misc_feature 5353..5360 /label=Linker 6 misc_feature 5361..8230 /label=pFun misc_feature 5353..8230 /label=BBa_K678046 ORIGIN 1 acgtcgctct ccccgatgac atacaggagg ggtcagggca ttcgagaaat accgtggata 61 cagttgggca tttctagggc tgaatgggaa ggagagagtt ttgaaatagg cgttccgttc 121 tgcttagggt atttgggaac aatcaatgtt caatgtacat ttaatccacg attttataaa 181 acgtcatcct ttgccctccc ttcttatttg ccaataccaa aaatcttact ccagtggttc 241 ggtaatcgca gagttaaatc tgggctcggt ggcagatctg cgatgctcca taaccgttca 301 gatgttgatt ggaactgggt ggggtagaca gctcgaagac cgagtgaacg tatacctaag 361 acactttgac acggccggaa cactgtaagt cccttcgtat ttctccgcct gtgtggagct 421 accatccaat aacccccagc tgaaaagctg attgtgatag ttcccacttg tccgtccgca 481 tcggcatccg cagctcggga tagttccgac ctaggattgg atgcatgcgg aaccgcacga 541 gggcggggcg gaaattgaca caccactcct ctccacgcac cgttcaagag gtacgcgtat 601 agagccgtat agagcagaga cggagcactt tctggtactg tccgcacggg atgtccgcac 661 ggagagccac aaacgagcgg ggccccgtac gtgctctcct accccaggat cgcatccccg 721 catagctgaa catctatata aagaccccca aggttctcag tctcaccaac atcatcaacc 781 aacaatcaac agttctctac tcagttaatt agaactcttc caatcctatc acctcgcctc 841 aaatactaga gagtgcgata tgtacctctc cacggtcctc ttcccactac tggctctgaa 901 ccttggtctc tcacacgcga gattcgtccg tgaaactctc gagctgacat gggaatacgg 961 atcacccaac ggcggtacgc cgcgcgagat ggtcttcacg aatggggagt atccaggtcc 1021 ggatctgata tttgatgaag atgatgatgt cgaggtacaa ccaagttttc accgtatgta 1081 catccgacaa cagagttgac aatcacaggt tcttgtcatc aataaccttc ccttcaatac 1141 aaccgttcac tggcatggtt tagagtgtgt gccctttccg cgtcccattt ctgcatggac 1201 ctaaaaattc ttgcattctc acacactctc tctagaatgc gagaaacacc tgaagcggac 1261 ggtgtcccgg gccttacgca aacaccaata gagcccggtg cgacgttcac gtatcggttc 1321 agagcgtatc cagctggtac gttctggtat cactcgcatt acaaggggct catgcaagat 1381 gggcaggtag gagcaatgta tattcggtac gtctgatcag attacacctt atctgcacga 1441 tgaagcccat tgaggcagtc ttattgatca tgtctcaata gccgcaagcc tgatgctttg 1501 cgaccctacg cggttataac ggaagacccg agagagttag cagaaataca gtacgcagaa 1561 gataatcctt atttgatgct tgctacggac tggacatatc ttacttcggc ggagtatcat 1621 aacatcgagg ttgagtctgg ctataacgtc ttgtaagctc gatttttcct ttacttcctg 1681 ttgcgaacta attcaattgc aaaagctgcg tggatagtct cctcatcaat ggtcgaggct 1741 cagtctactg tcccggatac caataccttg aggaagtgag cgacgacggc cttactgccg 1801 tcctcgaagg aacacatctg accgagaaag ggtaagtctc tgcacgcgct acgcccagtc 1861 aagattatat aaatactgat attgtatgat acatagctgc ctccaaccgg acctacataa 1921 tgtccaaggc gactacggcc cctggaacct ctctgctgtt cccacagaag tcgtctttaa 1981 ttgcaccccg tccagtgtcg agcccccggt gatctacgtc gatcccgaat tcaacggttg 2041 ggtcagcctt aatttcattg gcggcgctgc gcaaaaggcc ataacttttt ctgtcgacaa 2101 tcacccaatg tgggtatatg aagttgacgg acagtttgtt gaaccgcgtg aagtcgaaat 2161 ggttggcgta tactcgggag ctcggtacgc cgtcatgatc aagctagacc agacaccagg 2221 cgactacgcg attcggattg ccgtcaatgg cggcgatcag gttatgagcg tttacgccat 2281 cctctcgtat gtcaatcagg attggatcca cagggagaat gtgcccaagg ccgctatcgg 2341 accgcataca gatacagtgg gctacatgaa ctacggcggt ggcaacacat ctgcagatgt 2401 acgccagctt ctcttcacag aaaatctgcc ggcctttggc gtcccgcctc ccccaccttc 2461 gagcgaggtc tcaacaaccc tacgtaccgg catgatccgc gtaaacaact cctactcttg 2521 gtcattggga aacaacgtgc tgtacgagcc cgaaatgacc tcctcgacac cgctcctctt 2581 cgaaccggat ccgctagccg tcatcgcacc aaagtacgcc ctcacgacag aaaacaacac 2641 ctgggtcgac attgtcctcg aaatcactgc cgacccgcgc gacctcatcc acccgccaca 2701 tccgatccat aagcacggta acagagcata tataattggc aacggggttg gcaaattcag 2761 atgggagaat gtgagtgctg cagaggcaga agtaccggat ttgttttacg tcaacgagac 2821 tgcggcgttg cgggatacat ttgtcacgga tttttttgat tcgcggctca tggatggggc 2881 atggatagtt attcggtatt ttgtgcagga caagttccca agtatcttgc attgtcatat 2941 tgcgtcccat caaatgggag gaatggcgct ggccttgttg gacggggttg atgtttggga 3001 ttcttagtac tagagagcgc tggtatggcc tcctccgagg acgtcatcaa ggagttcatg 3061 cgcttcaagg tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag 3121 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc 3181 cccctgccct tcgcctggga catcctgtcc cctcagttcc agtacggctc caaggcctac 3241 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag 3301 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc 3361 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac 3421 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg gatgtacccc 3481 gaggacggcg ccctgaaggg cgagatcaag atgaggctga agctgaagga cggcggccac 3541 tacgacgccg aggtcaagac cacctacatg gccaagaagc ccgtgcagct gcccggcgcc 3601 tacaagaccg acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa 3661 cagtacgagc gcgccgaggg ccgccactcc accggcgcct aatactagag attccgatgg 3721 atccacttaa cgttactgaa atcatcaaac agcttgacga atctggatat aagatcgttg 3781 gtgtcgatgt cagctccgga gttgagacaa atggtgttca ggatctcgat aagatacgtt 3841 catttgtcca agcagcaaag agtgccttct agtgatttaa tagctccatg tcaacaagaa 3901 taaaacgcgt tttcgggttt acctcttcca gatacagctc atctgcaatg cattaatgca 3961 ttgactgcaa cctagtaacg cctttcaggc tccggcgaag agaagaatag cttagcagag 4021 ctattttcat tttcgggaga cgagatcaag cagatcaacg gtcgtcaaga gacctacgag 4081 actgaggaat ccgctcttgg ctccacgcga ctatatattt gtctctaatt gtactttgac 4141 atgctcctct tctttactct gatagcttga ctatgaaaat tccgtcacca gctcctgggt 4201 tcgcaaagat aattgcatgt ttcttccttg aactctcaag cctacaggac acacattcat 4261 cgtaggtata aacctcgaaa tcatttccta ctaagatggt atacaatagt aaccatgcat 4321 ggttgcctag tgaatgctcc gtaacaccca atacgccggc cgaaactttt ttacaactct 4381 cctatgagtc gtttacccag aatgcacagg tacacttgtt tagaggtaat ccttctttct 4441 agaagtcctc gtgtactgtg taagcgccct actagagact tgcgtaccaa tgcttaatca 4501 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 4561 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagcgct gcgatgatac 4621 cgcgagaacc acgctcaccg gctccggatt tatcagcaat aaaccagcca gccggaaggg 4681 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 4741 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccatcgcta 4801 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 4861 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 4921 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 4981 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 5041 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 5101 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 5161 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 5221 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 5281 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 5341 tcattactag agattaagct accgctaaga cctcagcgcg gccgcaaatt taaataaaat 5401 gaagtgaagt tcctatactt tctagagaat aggaacttct atagtgagtc gaataagggc 5461 gacacaaaat ttattctaaa tgcataataa atactgataa catcttatag tttgtattat 5521 attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt ccctttatta 5581 ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta tatacaaaaa 5641 atcataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa aagcaacgta 5701 tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc tcatatatca 5761 agcaaagtga caggcgccct taaatattct gacaaatgct ctttccctaa actcccccca 5821 taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg attaacgatt actcgttatc 5881 agaaccgccc agggggcccg agcttaagac tggccgtcgt tttacaacac agaaagagtt 5941 tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc ttagtttgat gcctggcagt 6001 tccctactct cgccttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 6061 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 6121 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 6181 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 6241 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 6301 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 6361 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 6421 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 6481 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 6541 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 6601 ttgaagtggt gggctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 6661 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 6721 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 6781 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga cgcgcgcgta 6841 actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca gcgtaatgct 6901 ctgcttttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 6961 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 7021 tggccccagc gctgcgatga taccgcgaga accacgctca ccggctccgg atttatcagc 7081 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 7141 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 7201 gcgcaacgtt gttgccatcg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 7261 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 7321 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 7381 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 7441 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7501 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 7561 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7621 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 7681 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 7741 ggcgacacgg aaatgttgaa tactcatatt cttccttttt caatattatt gaagcattta 7801 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 7861 aggggtcagt gttacaacca attaaccaat tctgaacatt atcgcgagcc catttatacc 7921 tgaatatggc tcataacacc ccttgtttgc ctggcggcag tagcgcggtg gtcccacctg 7981 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggactcccc 8041 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 8101 gcctttcgcc cgggctaatt atggggtgtc gcccttattc gactctatag tgaagttcct 8161 attctctaga aagtatagga acttctgaag tggggattta aatgcggccg cgctgagggt 8221 ttaatcgacg //