LOCUS BBa_K678070 12812 bp DNA linear 22-Dec-2024 DEFINITION p68 ACCESSION BBa_K678070 VERSION BBa_K678070.1 FEATURES Location/Qualifiers misc_feature 1..859 /label=ampR misc_feature 1318..3266 /label=IS-up misc_feature 3285..3318 /label=USER cassette (AsiSI/Nb.BtsI) CDS 3319..6392 /label=lacZ polyA_site 6396..7157 /label=TtrpC misc_feature 7171..9327 /label=argB marker cassette misc_feature 9342..11267 /label=IS-down ORIGIN 1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcga 1321 cgccagcagt gtcacaatcg accacgaccc agatcggttg tactttttgc gcgaagactc 1381 tgacacagca accagggaat ttattgatct tactgaggat gagacgacgc atgatggcga 1441 atacataacc gacgagtgtt tcgaggtact gcgcgaggac tggcttgctt ccgcgtgtgc 1501 ttccagtcct ctctcgccgg agcttccgga agggcagctg ctagaagagg tctgtgatgc 1561 tgacggaatt gtctacaagc cgggcaattc cgtcgagctc cacgatggcg cattcttccg 1621 aatttgctct atacggcaag cattgaaagg caatataata ttgaccggcc gccggttgct 1681 caaattcaaa gaccacccag acaaatacct tccccaatgg cgcaatgagc tgatttgggt 1741 tgccgatgaa actgcggaga ttcctctctg gttcgttcga cgttttgtct cagtacactt 1801 cagcaacgtt tgtcccattg gtcaagactg tcagaagaat aataatcctg atggcctatt 1861 ctgccgcttg aagagggtta ttcagaaaga gactacttca attgaatacc tgacattcga 1921 ggaagccgat gccgggttcc gcgcgccctc ggcaagcttg cgccatggtt ggcgggggga 1981 aaccgctcct ttcggcagca aagaggaagc tgagacgccg gtgattgtgt tagatgatga 2041 caatgacttt caggatacaa tattaaaaca gaaggcgcag cggaagtata ccttcggtga 2101 cggattctgt ggtgcgggtg gggtctcgtg cggcgctgaa gccgctggct tggacatcaa 2161 atgggccttt gatttatgcc cccacgcggc agcaacttat cgactgaatt ttcccaatgt 2221 agaatgtgag ggaagcgata ttttcagctt tatgaccagc aatgaggagt ttatgagagt 2281 ggacatctct catggctcgc ccccgtgtca aaccttctcg cctgctcaca caatcccagg 2341 gcccaatgat gatgccaact cagcagctat attctcatgc tgggacctca ttcgtaaagc 2401 aaaaccgaga gttcatacca tggaagaaac gagcggtctg ttcgatcggc atcaacaagt 2461 gttcctccgg gttatctgtg atttcataga gacagggtac tccgtccgat gggcccttct 2521 taattgtatg tggtatgggg tgccacagtc tcgaaagaga ttgattatca ttgcgtcagg 2581 gtatgcgtgc tcaagattcg atgctgactc atgactaaca tcggtccttt ttaggcctgg 2641 ggaaagcctc ccacggctgc caaggcctac tcatggcctg ccagggtccg ggctgcgtga 2701 tcttacaaca atcagccagg caattcgcga tatccccact ggaagtcccg atcacgacgt 2761 cgttgccgca cggggtcgcg gagttcataa tcgaagagct ccattcgacg gaaaccgaca 2821 agctcgcaca ataacatgcg gcggtgggga caattatcat ccctctggat tgcgcggttt 2881 taccctcagg gagtttgctt gtctgcaaac ctttcctctg ggctttcggt tcctgggcgg 2941 taggacgcag gtaaagaggc agatcggcaa tgcagtgcct cccctactgg ccaaagctgt 3001 ctttaaggaa atcatcaggt cgctgcaaga caccgatgag cgtgaattgc acgaaaacca 3061 atgatcgcca ttagggtcct gaggaaccac atgctctgga cctagccgcg ggattgcaga 3121 acgccgtcca accgtggaga cctcggcgag cgatcggaag cgataacacg ctggacaacg 3181 ggaagaggct cagtctgttc tcagccaaga cagatagtag tggcagatct tccaattgaa 3241 cgtttgatga ttgctggtct gtgtaaatta agtcctcagc gaatcgtgcg atgcagtgag 3301 agcgatcgca gacactgcat gaccatgatt acggattcac tggccgtcgt tttacaacgt 3361 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 3421 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 3481 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg 3541 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac 3601 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt 3661 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg 3721 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg 3781 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg 3841 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac 3901 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg 3961 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat 4021 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac 4081 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct 4141 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg 4201 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt 4261 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc 4321 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc 4381 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg 4441 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg 4501 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc 4561 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta 4621 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg 4681 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc 4741 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc 4801 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg 4861 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg 4921 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg 4981 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag 5041 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc 5101 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg 5161 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg 5221 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac 5281 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct 5341 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa 5401 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccggacac 5461 atcagcgcct ggcagcagtg gcgtctggct gaaaacctca gcgtgacact ccccgccgcg 5521 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat 5581 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa 5641 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt 5701 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg 5761 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg 5821 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg 5881 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg 5941 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag 6001 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc 6061 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc 6121 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc 6181 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc 6241 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt 6301 ggtggcgacg actcctggag cccgtcagta tcggcggaat tccagctgag cgccggtcgc 6361 taccattacc agttggtctg gtgtcaaaaa taaatcgcgt gcattcggat ccacttaacg 6421 ttactgaaat catcaaacag cttgacgaat ctggatataa gatcgttggt gtcgatgtca 6481 gctccggagt tgagacaaat ggtgttcagg atctcgataa gatacgttca tttgtccaag 6541 cagcaaagag tgccttctag tgatttaata gctccatgtc aacaagaata aaacgcgttt 6601 tcgggtttac ctcttccaga tacagctcat ctgcaatgca ttaatgcatt gactgcaacc 6661 tagtaacgcc tttcaggctc cggcgaagag aagaatagct tagcagagct attttcattt 6721 tcgggagacg agatcaagca gatcaacggt cgtcaagaga cctacgagac tgaggaatcc 6781 gctcttggct ccacgcgact atatatttgt ctctaattgt actttgacat gctcctcttc 6841 tttactctga tagcttgact atgaaaattc cgtcaccagc tcctgggttc gcaaagataa 6901 ttgcatgttt cttccttgaa ctctcaagcc tacaggacac acattcatcg taggtataaa 6961 cctcgaaatc atttcctact aagatggtat acaatagtaa ccatgcatgg ttgcctagtg 7021 aatgctccgt aacacccaat acgccggccg aaactttttt acaactctcc tatgagtcgt 7081 ttacccagaa tgcacaggta cacttgttta gaggtaatcc ttctttctag aagtcctcgt 7141 gtactgtgta agcgcccatc gcgtgcattc cgcggttttt tggggtagtc atctaatgaa 7201 acagacccgg acgcagcaga ggaagccccg cgatgactct ataccaccgt acgccgatat 7261 atcatcatcg cggcgatgga gaagtggggt tgactccgaa gacacttcaa aggagcgacg 7321 ctgttgattt gtagacgacg cttgataggg agaagcatta ttgtcgtgat gctcgcccaa 7381 cagaggccga ctcgcctcat ccgtcataac gaacgctgtg taaagcggag tgggggggaa 7441 agtgtggatt gtggagagta tgcgatagtg ttgaggctga tcagacggcg aatcgggcca 7501 gatatgacca gtttagaggc ctcatttgac tataatttac ataaattaga taaatagaga 7561 tgaacgcatg caataattgc agcaaatatt gatgaagcga gaggtaggac gatgaaggac 7621 tgtgagcagt tcaaggtatc agcagagtca agggcctgat gcaatggcgg tgatccgtga 7681 tcagcgaacg gaaggggcgc taactctgtt tctttaccaa tgatcggaag ctcctgctgg 7741 cggacttatg agtcattcac gaatcatttc tcagttattt gtggatgccc tcgttctgtc 7801 cacaatttct ttccgcccca agtcttttaa gttctttaac atctatattc ttgcacttcc 7861 aatggcatcc cttcgctccg tactcaagag ccagagcttg cgacacaccg tgcgatccta 7921 ctcctcgcaa accatgcctc ccgcctcacc ctttgctccc cgccacttcc tctccattgc 7981 ggacctctcg ccctccgagt tcgcaaccct tgttcgcaat gcctcctcac acaaacgggc 8041 tatcaagtcg gggtcaatgc cccaaaactt gcagggatca ctccttggga aaactgtggc 8101 catgatcttc agcaaacgaa gcacgaggac aagggtatct acagaagggg ccgttgtgca 8161 gatgggaggt catccgatgt tcttgggcaa ggatgatatc caactaggtg tcaacgagtc 8221 cctatacgac acctccgttg tcatttcgtc catggtatcc tgcattgtag cccgtgtcgg 8281 taaacatgca gaggtcgcag atctggcgaa gcactcttcg gttccagtca tcaatgcttt 8341 gtgtgactct ttccaccctc tccaagccgt ggccgatttc cagaccatct atgaagcatt 8401 cacccccaag gcgcaccacc tttcaagtct agggttggaa ggattgaaga tcgcttgggt 8461 gggtgacgcc aacaacgtcc tgttcgatat ggccattgct gctacaaaaa tgggtgtcga 8521 cattgctgtc gctactccca aggggtacga aatccctcct cacatgctgg agctcatcaa 8581 gtctgctgga gagggtgtct cgaaaccagg aaagcttctg caaaccaata ttcccgaaga 8641 agcggtcaag gacgccgata ttctggtcac agacacctgg gtctctatgg gccaagagga 8701 agagaaggct cagaggctga aggagtttga tggtttccaa atcactgctg aactcgccaa 8761 gcgaggagga gctaaggagg gctggaagtt catgcactgt ctcccgcgac accctgagga 8821 ggtcagcgac gaggttttct acagcaaccg gtcacttgtc ttccctgagg ctgagaaccg 8881 gttatgggct gcgatttccg ccttggaggg tttcgttgtc aataagggaa aaattgaata 8941 aatataacca ggcttccatt taaatatata gaggctggcg ttatcaaact gatgagttga 9001 cgggtatgag atcattccgt ccctaaatat atttactccg atcacgtaaa agcctgttag 9061 tagaagcatt ttcccaatta tcctgaccaa ttctttctag catatatcaa ataactaatt 9121 gacatgttct ctcgcttcct tatattactc agagtattgg aaatggggca aatcgcaccc 9181 ggtgactttc acatgtcacg aatgcggagt cgtcctagcc aaggtagatc caggcctaac 9241 acaccccaac cctcgactct cctcattcca tactcttgac ctctatccag cacattcttc 9301 tgaggtttcg caatggctgt aggtggcgct gaggcattaa tgcggttgat tgagtcgaga 9361 tagtcgagat ggccgggtca taggaacgct ggagtggtag atgcggggta cggtgggaag 9421 cgccatctgg ggcacccgtt gaccagcttg gaagggccga aaagcgctac aatcactaaa 9481 gagcacatta aggcgagtcc gattcaaagg acgtaaagca gcttgagcaa gatgccaaat 9541 tccgagatcg gatggtaaaa tcaccgagtg taaccggcga gcagctatgt actccttacg 9601 gtgtgcgggt aaatgcccgg cccagaggcg taaaagccac ggcctcggct cagtcggcca 9661 accgtcaatc cctccctccc agagactcag ccgacccttc ttcttctctc cctctccctt 9721 ccttccgacc aggcgatttc tttcttctcc ttctgacatc aattgggtat tgcaatctca 9781 atcctatctc tgtgtccttt ctcttctctt cactatgtct gcctcttcag cgctgagacg 9841 gctgacccac aggtctcccc gcaatctcca gtcccgcatc gcttcgctct cccgcacctc 9901 ttgttcccct tcgtcgacgt tcaactccta cagagcttct tcatctcctg ctttacctcg 9961 tgcttcgaga ttctcaacaa tggcttctct acgatcggcg gcgccaattc ccgcgtcctc 10021 ccaagtggac tatgatcctg aaatcaagga catggccgac tacatccaca actacaagat 10081 cgactctgat ctcgcggtat gagctccaat tgagaaccca attatggatt gatggggtaa 10141 tacgcaattg gtactgatta aatacagttc gacaccgccc gcctggttct ccttgatact 10201 cttggatgtg gtctggaagc tctgaagttc aaggagtgca ccaagctcct tggaccggtt 10261 gttgatggca ctgtcgttcc taacggcact cgcgttcctg gtactcccta ccagctagac 10321 cctgtaaatg gtgccttcaa catcggtgca atgatccgct ggctcgatta caatgactgc 10381 tggctcgctg ctgagtgggg tcatccttcc gacaacttgg gcggtattct cgcagtcgct 10441 gactgggtct ctcgtaccaa ccgcgccggt ggaaacatcg ccggtggtaa gatcttcacc 10501 atcaaggaag tcttggaggc aatgatcaag gcccacgaaa tccagggtgt tctcgccttg 10561 gagaactcct acaacaaggt cggtctggac cacgttgttt tggtcaaggt tgccaccacc 10621 gctgttgtgt ccaagatgct tggtctcagc gagaagcaga ccgccgacgc catcacccag 10681 gcgtgggttg acggtcagag tctccgcacc tacagacact cccccaacac catgtctagg 10741 aagtcctggg ctgccggtga cgcctgccag cgcgccgtta accttgttct caaggttcaa 10801 aagggtgagg gcggtctcaa gaccgtcctc tctgcccccg tctggggttt ctatgatgtc 10861 ctgttcaagg gcaacaagtt caagttccag cgcccatacg gcagctacgt tatggagaac 10921 gttcttttca aggtttccta tcccggtatg tgtcccttgc ctcgctcgtg cctagggggt 10981 tgctaatgtg tgtaaagccg aattccactc tcagactgct attgaggctg ccgagatcat 11041 caacaagaag ctcgccgccc tcggcaagag cgccaaagat atcaaggagg tcactaaccg 11101 cacccacgag gcgtgcatcc gcatcatcga caagcagttc aaggctatgg acaactttgc 11161 ggaccgtgac cactgcgttc aggtatgaca tcttaaacac gaatgagaag ttggcaaata 11221 ctgattgcac tcagtacatg gtggctacta tgctcgtctt caaccgcatt aagacctcag 11281 cgcggccgca aatttaaata aaatgaagtg aagttcctat actttctaga gaataggaac 11341 ttctatagtg agtcgaataa gggcgacaca aaatttattc taaatgcata ataaatactg 11401 ataacatctt atagtttgta ttatattttg tattatcgtt gacatgtata attttgatat 11461 caaaaactga ttttcccttt attattttcg agatttattt tcttaattct ctttaacaaa 11521 ctagaaatat tgtatataca aaaaatcata aataatagat gaatagttta attataggtg 11581 ttcatcaatc gaaaaagcaa cgtatcttat ttaaagtgcg ttgctttttt ctcatttata 11641 aggttaaata attctcatat atcaagcaaa gtgacaggcg cccttaaata ttctgacaaa 11701 tgctctttcc ctaaactccc cccataaaaa aacccgccga agcgggtttt tacgttattt 11761 gcggattaac gattactcgt tatcagaacc gcccaggggg cccgagctta agactggccg 11821 tcgttttaca acacagaaag agtttgtaga aacgcaaaaa ggccatccgt caggggcctt 11881 ctgcttagtt tgatgcctgg cagttcccta ctctcgcctt ccgcttcctc gctcactgac 11941 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 12001 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 12061 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 12121 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 12181 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 12241 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 12301 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 12361 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 12421 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 12481 tatgtaggcg gtgctacaga gttcttgaag tggtgggcta actacggcta cactagaaga 12541 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 12601 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 12661 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 12721 gctcagtgga acgacgcgcg cgtaactcac gttaagggat tttggtcatg agcttgcgcc 12781 gtcccgtcaa gtcagcgtaa tgctctgctt tt //