##gff-version 3
##sequence-region BBa_K678070 1 11267
BBa_K678070	.	CDS	3319	6392	.	+	0	ID=annotation2135108;Name=lacZ
BBa_K678070	.	sequence_feature	1	859	.	+	0	ID=annotation2135105;Name=ampR
BBa_K678070	.	sequence_feature	3285	3318	.	+	0	ID=annotation2135107;Name=USER cassette (AsiSI/Nb.BtsI)
BBa_K678070	.	sequence_feature	9342	11267	.	+	0	ID=annotation2135111;Name=IS-down
BBa_K678070	.	polyA_site	6396	7157	.	+	0	ID=annotation2135109;Name=TtrpC
BBa_K678070	.	sequence_feature	1318	3266	.	+	0	ID=annotation2135106;Name=IS-up
BBa_K678070	.	sequence_feature	7171	9327	.	+	0	ID=annotation2135110;Name=argB marker cassette
>BBa_K678070
accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgt
gtagataactacgatacgggagggcttaccatctggccccagcgctgcgatgataccgcgagaaccacgctcaccggct
ccggatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatcc
agtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccatcgctac
aggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatga
tcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttat
cactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagta
ctcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcg
ccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgt
tgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtg
agcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctt
tttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaac
aaataggggtcagtgttacaaccaattaaccaattctgaacattatcgcgagcccatttatacctgaatatggctcata
acaccccttgtttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtag
cgccgatggtagtgtggggactccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgcccgggctaattatggggtgtcgcccttattcgactctatagtgaagttcctattctctagaaa
gtataggaacttctgaagtggggatttaaatgcggccgcgctgagggtttaatcgacgccagcagtgtcacaatcgacc
acgacccagatcggttgtactttttgcgcgaagactctgacacagcaaccagggaatttattgatcttactgaggatga
gacgacgcatgatggcgaatacataaccgacgagtgtttcgaggtactgcgcgaggactggcttgcttccgcgtgtgct
tccagtcctctctcgccggagcttccggaagggcagctgctagaagaggtctgtgatgctgacggaattgtctacaagc
cgggcaattccgtcgagctccacgatggcgcattcttccgaatttgctctatacggcaagcattgaaaggcaatataat
attgaccggccgccggttgctcaaattcaaagaccacccagacaaataccttccccaatggcgcaatgagctgatttgg
gttgccgatgaaactgcggagattcctctctggttcgttcgacgttttgtctcagtacacttcagcaacgtttgtccca
ttggtcaagactgtcagaagaataataatcctgatggcctattctgccgcttgaagagggttattcagaaagagactac
ttcaattgaatacctgacattcgaggaagccgatgccgggttccgcgcgccctcggcaagcttgcgccatggttggcgg
ggggaaaccgctcctttcggcagcaaagaggaagctgagacgccggtgattgtgttagatgatgacaatgactttcagg
atacaatattaaaacagaaggcgcagcggaagtataccttcggtgacggattctgtggtgcgggtggggtctcgtgcgg
cgctgaagccgctggcttggacatcaaatgggcctttgatttatgcccccacgcggcagcaacttatcgactgaatttt
cccaatgtagaatgtgagggaagcgatattttcagctttatgaccagcaatgaggagtttatgagagtggacatctctc
atggctcgcccccgtgtcaaaccttctcgcctgctcacacaatcccagggcccaatgatgatgccaactcagcagctat
attctcatgctgggacctcattcgtaaagcaaaaccgagagttcataccatggaagaaacgagcggtctgttcgatcgg
catcaacaagtgttcctccgggttatctgtgatttcatagagacagggtactccgtccgatgggcccttcttaattgta
tgtggtatggggtgccacagtctcgaaagagattgattatcattgcgtcagggtatgcgtgctcaagattcgatgctga
ctcatgactaacatcggtcctttttaggcctggggaaagcctcccacggctgccaaggcctactcatggcctgccaggg
tccgggctgcgtgatcttacaacaatcagccaggcaattcgcgatatccccactggaagtcccgatcacgacgtcgttg
ccgcacggggtcgcggagttcataatcgaagagctccattcgacggaaaccgacaagctcgcacaataacatgcggcgg
tggggacaattatcatccctctggattgcgcggttttaccctcagggagtttgcttgtctgcaaacctttcctctgggc
tttcggttcctgggcggtaggacgcaggtaaagaggcagatcggcaatgcagtgcctcccctactggccaaagctgtct
ttaaggaaatcatcaggtcgctgcaagacaccgatgagcgtgaattgcacgaaaaccaatgatcgccattagggtcctg
aggaaccacatgctctggacctagccgcgggattgcagaacgccgtccaaccgtggagacctcggcgagcgatcggaag
cgataacacgctggacaacgggaagaggctcagtctgttctcagccaagacagatagtagtggcagatcttccaattga
acgtttgatgattgctggtctgtgtaaattaagtcctcagcgaatcgtgcgatgcagtgagagcgatcgcagacactgc
atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatc
gccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcg
cagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatctt
cctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacct
atcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatga
aagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgc
tgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcc
tcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccg
tgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagc
cgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagg
gtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgt
cacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactg
cacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgc
tgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatgga
tgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaac
catccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatgg
tgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcga
tcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgc
tggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattattt
gcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgct
acctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatac
tggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatg
atgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacgg
tctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgttta
tccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtggcgc
tggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctga
actaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaa
gccggacacatcagcgcctggcagcagtggcgtctggctgaaaacctcagcgtgacactccccgccgcgtcccacgcca
tcccgcatctgaccaccagcgaaatggatttttgcatcgagctgggtaataagcgttggcaatttaaccgccagtcagg
ctttctttcacagatgtggattggcgataaaaaacaactgctgacgccgctgcgcgatcagttcacccgtgcaccgctg
gataacgacattggcgtaagtgaagcgacccgcattgaccctaacgcctgggtcgaacgctggaaggcggcgggccatt
accaggccgaagcagcgttgttgcagtgcacggcagatacacttgctgatgcggtgctgattacgaccgctcacgcgtg
gcagcatcaggggaaaaccttatttatcagccggaaaacctaccggattgatggtagtggtcaaatggcgattaccgtt
gatgttgaagtggcgagcgatacaccgcatccggcgcggattggcctgaactgccagctggcgcaggtagcagagcggg
taaactggctcggattagggccgcaagaaaactatcccgaccgccttactgccgcctgttttgaccgctgggatctgcc
attgtcagacatgtataccccgtacgtcttcccgagcgaaaacggtctgcgctgcgggacgcgcgaattgaattatggc
ccacaccagtggcgcggcgacttccagttcaacatcagccgctacagtcaacagcaactgatggaaaccagccatcgcc
atctgctgcacgcggaagaaggcacatggctgaatatcgacggtttccatatggggattggtggcgacgactcctggag
cccgtcagtatcggcggaattccagctgagcgccggtcgctaccattaccagttggtctggtgtcaaaaataaatcgcg
tgcattcggatccacttaacgttactgaaatcatcaaacagcttgacgaatctggatataagatcgttggtgtcgatgt
cagctccggagttgagacaaatggtgttcaggatctcgataagatacgttcatttgtccaagcagcaaagagtgccttc
tagtgatttaatagctccatgtcaacaagaataaaacgcgttttcgggtttacctcttccagatacagctcatctgcaa
tgcattaatgcattgactgcaacctagtaacgcctttcaggctccggcgaagagaagaatagcttagcagagctatttt
cattttcgggagacgagatcaagcagatcaacggtcgtcaagagacctacgagactgaggaatccgctcttggctccac
gcgactatatatttgtctctaattgtactttgacatgctcctcttctttactctgatagcttgactatgaaaattccgt
caccagctcctgggttcgcaaagataattgcatgtttcttccttgaactctcaagcctacaggacacacattcatcgta
ggtataaacctcgaaatcatttcctactaagatggtatacaatagtaaccatgcatggttgcctagtgaatgctccgta
acacccaatacgccggccgaaacttttttacaactctcctatgagtcgtttacccagaatgcacaggtacacttgttta
gaggtaatccttctttctagaagtcctcgtgtactgtgtaagcgcccatcgcgtgcattccgcggttttttggggtagt
catctaatgaaacagacccggacgcagcagaggaagccccgcgatgactctataccaccgtacgccgatatatcatcat
cgcggcgatggagaagtggggttgactccgaagacacttcaaaggagcgacgctgttgatttgtagacgacgcttgata
gggagaagcattattgtcgtgatgctcgcccaacagaggccgactcgcctcatccgtcataacgaacgctgtgtaaagc
ggagtgggggggaaagtgtggattgtggagagtatgcgatagtgttgaggctgatcagacggcgaatcgggccagatat
gaccagtttagaggcctcatttgactataatttacataaattagataaatagagatgaacgcatgcaataattgcagca
aatattgatgaagcgagaggtaggacgatgaaggactgtgagcagttcaaggtatcagcagagtcaagggcctgatgca
atggcggtgatccgtgatcagcgaacggaaggggcgctaactctgtttctttaccaatgatcggaagctcctgctggcg
gacttatgagtcattcacgaatcatttctcagttatttgtggatgccctcgttctgtccacaatttctttccgccccaa
gtcttttaagttctttaacatctatattcttgcacttccaatggcatcccttcgctccgtactcaagagccagagcttg
cgacacaccgtgcgatcctactcctcgcaaaccatgcctcccgcctcaccctttgctccccgccacttcctctccattg
cggacctctcgccctccgagttcgcaacccttgttcgcaatgcctcctcacacaaacgggctatcaagtcggggtcaat
gccccaaaacttgcagggatcactccttgggaaaactgtggccatgatcttcagcaaacgaagcacgaggacaagggta
tctacagaaggggccgttgtgcagatgggaggtcatccgatgttcttgggcaaggatgatatccaactaggtgtcaacg
agtccctatacgacacctccgttgtcatttcgtccatggtatcctgcattgtagcccgtgtcggtaaacatgcagaggt
cgcagatctggcgaagcactcttcggttccagtcatcaatgctttgtgtgactctttccaccctctccaagccgtggcc
gatttccagaccatctatgaagcattcacccccaaggcgcaccacctttcaagtctagggttggaaggattgaagatcg
cttgggtgggtgacgccaacaacgtcctgttcgatatggccattgctgctacaaaaatgggtgtcgacattgctgtcgc
tactcccaaggggtacgaaatccctcctcacatgctggagctcatcaagtctgctggagagggtgtctcgaaaccagga
aagcttctgcaaaccaatattcccgaagaagcggtcaaggacgccgatattctggtcacagacacctgggtctctatgg
gccaagaggaagagaaggctcagaggctgaaggagtttgatggtttccaaatcactgctgaactcgccaagcgaggagg
agctaaggagggctggaagttcatgcactgtctcccgcgacaccctgaggaggtcagcgacgaggttttctacagcaac
cggtcacttgtcttccctgaggctgagaaccggttatgggctgcgatttccgccttggagggtttcgttgtcaataagg
gaaaaattgaataaatataaccaggcttccatttaaatatatagaggctggcgttatcaaactgatgagttgacgggta
tgagatcattccgtccctaaatatatttactccgatcacgtaaaagcctgttagtagaagcattttcccaattatcctg
accaattctttctagcatatatcaaataactaattgacatgttctctcgcttccttatattactcagagtattggaaat
ggggcaaatcgcacccggtgactttcacatgtcacgaatgcggagtcgtcctagccaaggtagatccaggcctaacaca
ccccaaccctcgactctcctcattccatactcttgacctctatccagcacattcttctgaggtttcgcaatggctgtag
gtggcgctgaggcattaatgcggttgattgagtcgagatagtcgagatggccgggtcataggaacgctggagtggtaga
tgcggggtacggtgggaagcgccatctggggcacccgttgaccagcttggaagggccgaaaagcgctacaatcactaaa
gagcacattaaggcgagtccgattcaaaggacgtaaagcagcttgagcaagatgccaaattccgagatcggatggtaaa
atcaccgagtgtaaccggcgagcagctatgtactccttacggtgtgcgggtaaatgcccggcccagaggcgtaaaagcc
acggcctcggctcagtcggccaaccgtcaatccctccctcccagagactcagccgacccttcttcttctctccctctcc
cttccttccgaccaggcgatttctttcttctccttctgacatcaattgggtattgcaatctcaatcctatctctgtgtc
ctttctcttctcttcactatgtctgcctcttcagcgctgagacggctgacccacaggtctccccgcaatctccagtccc
gcatcgcttcgctctcccgcacctcttgttccccttcgtcgacgttcaactcctacagagcttcttcatctcctgcttt
acctcgtgcttcgagattctcaacaatggcttctctacgatcggcggcgccaattcccgcgtcctcccaagtggactat
gatcctgaaatcaaggacatggccgactacatccacaactacaagatcgactctgatctcgcggtatgagctccaattg
agaacccaattatggattgatggggtaatacgcaattggtactgattaaatacagttcgacaccgcccgcctggttctc
cttgatactcttggatgtggtctggaagctctgaagttcaaggagtgcaccaagctccttggaccggttgttgatggca
ctgtcgttcctaacggcactcgcgttcctggtactccctaccagctagaccctgtaaatggtgccttcaacatcggtgc
aatgatccgctggctcgattacaatgactgctggctcgctgctgagtggggtcatccttccgacaacttgggcggtatt
ctcgcagtcgctgactgggtctctcgtaccaaccgcgccggtggaaacatcgccggtggtaagatcttcaccatcaagg
aagtcttggaggcaatgatcaaggcccacgaaatccagggtgttctcgccttggagaactcctacaacaaggtcggtct
ggaccacgttgttttggtcaaggttgccaccaccgctgttgtgtccaagatgcttggtctcagcgagaagcagaccgcc
gacgccatcacccaggcgtgggttgacggtcagagtctccgcacctacagacactcccccaacaccatgtctaggaagt
cctgggctgccggtgacgcctgccagcgcgccgttaaccttgttctcaaggttcaaaagggtgagggcggtctcaagac
cgtcctctctgcccccgtctggggtttctatgatgtcctgttcaagggcaacaagttcaagttccagcgcccatacggc
agctacgttatggagaacgttcttttcaaggtttcctatcccggtatgtgtcccttgcctcgctcgtgcctagggggtt
gctaatgtgtgtaaagccgaattccactctcagactgctattgaggctgccgagatcatcaacaagaagctcgccgccc
tcggcaagagcgccaaagatatcaaggaggtcactaaccgcacccacgaggcgtgcatccgcatcatcgacaagcagtt
caaggctatggacaactttgcggaccgtgaccactgcgttcaggtatgacatcttaaacacgaatgagaagttggcaaa
tactgattgcactcagtacatggtggctactatgctcgtcttcaaccgcattaagacctcagcgcggccgcaaatttaa
ataaaatgaagtgaagttcctatactttctagagaataggaacttctatagtgagtcgaataagggcgacacaaaattt
attctaaatgcataataaatactgataacatcttatagtttgtattatattttgtattatcgttgacatgtataatttt
gatatcaaaaactgattttccctttattattttcgagatttattttcttaattctctttaacaaactagaaatattgta
tatacaaaaaatcataaataatagatgaatagtttaattataggtgttcatcaatcgaaaaagcaacgtatcttattta
aagtgcgttgcttttttctcatttataaggttaaataattctcatatatcaagcaaagtgacaggcgcccttaaatatt
ctgacaaatgctctttccctaaactccccccataaaaaaacccgccgaagcgggtttttacgttatttgcggattaacg
attactcgttatcagaaccgcccagggggcccgagcttaagactggccgtcgttttacaacacagaaagagtttgtaga
aacgcaaaaaggccatccgtcaggggccttctgcttagtttgatgcctggcagttccctactctcgccttccgcttcct
cgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatc
cacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcg
ttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacc
cgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttac
cggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtg
taggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatc
gtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggta
tgtaggcggtgctacagagttcttgaagtggtgggctaactacggctacactagaagaacagtatttggtatctgcgct
ctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtt
tttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctga
cgctcagtggaacgacgcgcgcgtaactcacgttaagggattttggtcatgagcttgcgccgtcccgtcaagtcagcgt
aatgctctgctttt