BBa_K726003
1
BBa_K726003
T7 promoter + lac operator + His tag
2012-08-08T11:00:00Z
2015-05-08T01:13:05Z
The sequence is from pCDFDuet from Novagen.
T7 promoter is good for protein expression in <i>e. coli</i>. Requires the T7 RNA polymerase. Also contains a RBS, and a His tag for protein purification. Insert your protein of interest behind this.
false
false
_972_
0
10304
9
Not in stock
false
none.
false
Sami Chu
annotation2179384
1
T7 promoter
range2179384
1
1
17
annotation2179473
1
lac operator
range2179473
1
22
42
annotation2179385
1
His tag
range2179385
1
100
117
BBa_K726007
1
BBa_K726007
T7 driven lac operated inducer for the las quorum-sensing system
2012-08-09T11:00:00Z
2015-05-08T01:13:05Z
LacI is from the parts registry distribution plates, and the T7 promoter+lac operator is from the plasmid pCDFDuet from Novagen.
Produces chemical system PAI-1. Induces the promoter pLasR with the lasR regulatory protein. Requires T7 RNA polymerase and inducable by lactose.
Part of a two-strain self-inducing system.
false
false
_972_
0
10304
9
Not in stock
false
None.
false
Sami Chu
component2244963
1
BBa_C0078
component2244959
1
BBa_K726003
annotation2244963
1
BBa_C0078
range2244963
1
136
802
annotation2244959
1
BBa_K726003
range2244959
1
1
129
BBa_C0078
1
lasI
autoinducer synthetase for PAI from Pseudomonas aeruginosa
2004-01-27T12:00:00Z
2015-08-31T04:07:24Z
www.ncbi.nlm.nih.gov
Released HQ 2013
coding region for lasI protein, which produces the chemical signal AI-1
false
false
_1_
0
24
7
In stock
false
true
Chris Walsh (Fighting Darwins)
annotation306608
1
LVA
range306608
1
604
636
annotation305970
1
LasI
range305970
1
1
603
annotation2214003
1
Help:Barcodes
range2214003
1
643
667
BBa_C0078_sequence
1
atgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc
BBa_K726003_sequence
1
taatacgactcactataggggaattgtgagcggataacaattcccctgtagaaataattttgtttaactttaataaggagatataccatgggcagcagccatcaccatcatcaccacagccaggatccg
BBa_K726007_sequence
1
taatacgactcactataggggaattgtgagcggataacaattcccctgtagaaataattttgtttaactttaataaggagatataccatgggcagcagccatcaccatcatcaccacagccaggatccgtactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z