LOCUS BBa_K726016 4990 bp DNA linear 15-Nov-2024 DEFINITION T7Prom+HisTag+VioABE ACCESSION BBa_K726016 VERSION BBa_K726016.1 FEATURES Location/Qualifiers promoter 1..17 /label=T7 promoter misc_feature 100..117 /label=His tag misc_feature 22..42 /label=lac operator promoter 1..129 /label=BBa_K726003 CDS 136..1390 /label=VioA RBS 1391..1409 /label=RBS CDS 1410..4402 /label=VioB RBS 4402..4420 /label=RBS CDS 4420..4989 /label=VioE CDS 136..4990 /label=BBa_K726015 ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt 61 tgtttaactt taataaggag atataccatg ggcagcagcc atcaccatca tcaccacagc 121 caggatccgt actagatgaa acattcttcc gatatctgca ttgttggtgc tggtatttct 181 ggtttgacgt gcgcaagcca tctgctggac agcccggcat gccgtggtct gagcctgcgt 241 atctttgaca tgcagcaaga agccggtggc cgtatccgca gcaaaatgct ggatggtaag 301 gcaagcattg aactgggcgc aggtcgctac tcccctcagt tgcacccgca tttccaaagc 361 gcaatgcagc actatagcca aaagagcgaa gtctatccgt tcacccagtt gaagttcaaa 421 tctcacgtgc agcaaaagct gaagcgcgcc atgaatgaac tgtccccgcg tctgaaagag 481 catggtaaag agagcttttt gcagtttgtc agccgttatc aaggtcacga tagcgcggtt 541 ggtatgatcc gctctatggg ttacgacgca ctgttcctgc cggatatcag cgcagaaatg 601 gcctacgaca ttgtgggtaa gcacccggag atccagagcg tgacggacaa cgacgcgaac 661 caatggtttg cagcggaaac gggctttgct ggtctgattc agggcatcaa ggctaaggtt 721 aaggcggcag gtgcgcgttt tagcctgggt tatcgtctgc tgagcgtccg taccgacggt 781 gacggctacc tgctgcaact ggcaggtgac gacggctgga aactggagca ccgtacccgc 841 catctgattc tggcgattcc gccgagcgcg atggcgggtt tgaatgttga ttttccagaa 901 gcctggtccg gtgcgcgcta tggcagcctg ccgctgttta agggctttct gacgtacggt 961 gagccgtggt ggttggacta caaactggac gatcaggtgc tgattgttga caacccgctg 1021 cgcaaaatct atttcaaagg cgataagtac ctgttcttct ataccgatag cgagatggcg 1081 aattactggc gcggttgtgt cgcggagggc gaggacggtt acctggagca aattcgcacc 1141 catttggcta gcgcactggg tatcgtccgt gaacgtatcc cgcaaccgct ggcacacgtt 1201 cacaagtatt gggcgcacgg cgttgagttt tgccgtgatt ctgatattga ccacccgagc 1261 gcactgtctc atcgcgacag cggtatcatc gcgtgctccg atgcgtacac ggagcattgt 1321 ggttggatgg agggcggtct gctgagcgcc cgtgaggcaa gccgtctgct gttgcagcgt 1381 atcgccgcgt taaggaggta aaaaaaatga gcattctgga tttcccgcgt atccacttcc 1441 gtggctgggc ccgtgtcaat gcgccgaccg cgaaccgcga tccgcacggc cacatcgata 1501 tggccagcaa taccgtggcg atggcgggtg agccgttcga cctggcacgc catcctacgg 1561 agttccaccg tcacctgcgc tccctgggtc cgcgcttcgg cttggatggt cgtgctgacc 1621 cggaaggccc gttcagcctg gccgagggct acaacgctgc cggtaacaac cacttttcgt 1681 gggagagcgc aaccgttagc cacgtgcaat gggatggcgg tgaggcggat cgtggtgacg 1741 gtctggtcgg tgctcgtttg gcactgtggg gtcactacaa tgattatctg cgtaccacct 1801 tcaatcgtgc tcgttgggtc gacagcgacc cgacgcgccg tgacgctgca caaatctatg 1861 cgggccaatt caccattagc ccggctggtg ccggtccggg tacgccgtgg ctgtttacgg 1921 cagacattga tgatagccat ggtgcacgtt ggacgcgtgg cggccacatt gcagagcgtg 1981 gcggccactt cttggatgaa gagtttggtc tggcacgcct gtttcagttc tctgtgccga 2041 aagatcaccc acattttctg tttcacccgg gtccgtttga ttccgaggcc tggcgtcgtc 2101 tgcaattggc tctggaggat gacgacgttc tgggtctgac cgtgcaatat gcgttgttca 2161 atatgagcac cccgcctcag ccgaacagcc cggtttttca cgatatggtc ggtgttgtcg 2221 gtctgtggcg tcgtggtgaa ctggcgagct acccggctgg tcgtctgctg cgtccgcgtc 2281 aaccgggtct gggtgacctg accctgcgcg tcaacggtgg tcgcgttgcg ctgaatttgg 2341 cgtgtgccat tccgttcagc actcgtgccg cgcagccaag cgcaccggac cgcctgaccc 2401 cggacctggg tgccaaactg ccgctgggcg atctgctgct gcgtgatgag gacggcgcac 2461 tgttggcacg tgtgccgcag gctctgtacc aagactattg gacgaatcac ggtattgtgg 2521 acctgccgct gctgcgcgaa ccgcgtggta gcttgaccct gagcagcgaa ctggcggagt 2581 ggcgtgagca agactgggtc acccaaagcg acgcgtctaa cctgtacctg gaggcaccgg 2641 atcgccgtca cggtcgcttt ttccctgaga gcatcgcgct gcgcagctac tttcgcggtg 2701 aagcgcgtgc gcgtccggat atcccgcatc gtatcgaggg catgggcctg gtcggcgtcg 2761 aatctcgtca ggatggcgac gctgcggaat ggcgtctgac gggtctgcgt ccgggtccgg 2821 cacgcattgt tctggacgat ggtgccgagg cgatccctct gcgtgttctg cctgacgatt 2881 gggcgctgga tgacgcgacc gtcgaagaag tggattacgc ctttttgtac cgccacgtta 2941 tggcgtatta cgagctggtg tatccattca tgagcgacaa ggtgttttcc ctggctgatc 3001 gttgcaaatg tgaaacgtac gcacgtctga tgtggcagat gtgtgatccg cagaaccgca 3061 acaagtccta ttacatgccg agcacccgcg aactgtcggc accgaaagct cgtttgttct 3121 tgaagtatct ggcccacgtg gaaggccagg cacgcctgca agcacctccg ccagcgggtc 3181 cggcacgcat tgaatctaaa gcccagttgg cggcagagct gcgtaaagcc gtcgacctgg 3241 agctgtctgt gatgctgcaa tacctgtacg cggcgtatag cattccgaac tatgcacagg 3301 gccaacaacg tgttcgtgac ggtgcgtgga ccgccgagca gctgcaactg gcgtgcggta 3361 gcggtgaccg tcgccgtgat ggcggtattc gtgcagcact gctggaaatt gctcatgaag 3421 aaatgattca ttacctggtc gttaacaacc tgctgatggc cctgggcgag ccgttctacg 3481 cgggtgtccc gctgatgggc gaagcggcac gtcaggcgtt tggcctggac accgagttcg 3541 ctctggaacc gtttagcgaa agcacgctgg cacgttttgt tcgtctggaa tggccgcact 3601 ttatcccagc accgggcaaa tccatcgcgg actgctatgc cgccattcgt caggcgtttt 3661 tggatctgcc ggacttgttt ggtggcgagg caggtaagcg tggcggtgaa caccacctgt 3721 tcctgaatga gctgaccaac cgtgcgcatc cgggttatca actggaagtt ttcgatcgcg 3781 actcggcgct gtttggtatt gcatttgtga ccgatcaggg cgaaggtggc gctctggaca 3841 gcccgcacta cgaacatagc cattttcaac gtctgcgtga aatgagcgcg cgtatcatgg 3901 ctcaaagcgc accgttcgaa ccggcgctgc cggcgttgcg taatccggtt ctggatgaga 3961 gcccgggttg ccaacgtgtc gcagacggtc gtgcgcgtgc gctgatggca ttgtaccaag 4021 gcgtttatga gctgatgttt gcgatgatgg cgcagcactt cgccgtgaaa ccgctgggta 4081 gcttgcgtcg cagccgcctg atgaacgcag caatcgatct gatgaccggt ctgttgcgtc 4141 cgctgagctg cgcgctgatg aacctgccaa gcggcatcgc cggtcgcacg gccggtccgc 4201 cgctgccggg tccggttgac acccgtagct atgacgacta cgcgctgggc tgtcgcatgc 4261 tggcacgccg ttgcgagcgt ctgctggagc aggcgagcat gctggaaccg ggttggctgc 4321 cggatgcgca gatggagctg ctggatttct atcgtcgcca aatgctggac ttggcgtgcg 4381 gcaaactgag ccgcgaggcc ttaaggaggt aaaaaaaatg gagaaccgtg agccaccact 4441 gttgccagcc cgttggagca gcgcctatgt ctcttattgg agcccgatgc tgccggatga 4501 ccagctgacc agcggctatt gctggttcga ctatgaacgt gacatctgtc gtattgacgg 4561 cctgttcaat ccgtggagcg agcgtgatac tggttatcgc ctgtggatgt cggaggttgg 4621 taatgcggcc agcggccgta cctggaaaca aaaagtcgcc tatggtcgtg agcgtaccgc 4681 cctgggtgaa cagctgtgtg agcgtccgct ggatgatgag actggccctt ttgccgaatt 4741 gttcctgcca cgcgatgtcc tgcgccgtct gggtgcccgt cacattggcc gtcgcgtggt 4801 tctgggtcgc gaagcggacg gttggcgtta ccagcgccca ggtaaaggtc cgagcaccct 4861 gtacctggat gcggcgagcg gcactccact gcgcatggtc accggcgatg aagcgtcgcg 4921 tgcaagcctg cgtgattttc cgaatgtgag cgaggcggag atcccggacg cggttttcgc 4981 ggccaagcgc //