LOCUS       BBa_K726018             2576 bp    DNA     linear       01-Jun-2024
DEFINITION  T7Prom+HisTag+VioDC
ACCESSION   BBa_K726018
VERSION     BBa_K726018.1  
FEATURES             Location/Qualifiers
     promoter        1..17
                     /label=T7 promoter
     misc_feature    100..117
                     /label=His tag
     misc_feature    22..42
                     /label=lac operator 
     promoter        1..129
                     /label=BBa_K726003
     CDS             136..1264
                     /label=VioD
     RBS             1264..1281
                     /label=RBS
     CDS             1282..2576
                     /label=VioC
     CDS             136..2576
                     /label=BBa_K726017
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt
       61 tgtttaactt taataaggag atataccatg ggcagcagcc atcaccatca tcaccacagc
      121 caggatccgt actagatgaa gattctggtc attggtgctg gtccagctgg tctggttttc
      181 gcatcccaac tgaagcaggc acgccctttg tgggccattg acatcgtgga gaagaatgac
      241 gagcaagaag tgctgggctg gggtgtcgtg ctgcctggcc gtccgggtca gcacccggcg
      301 aacccgctgt cctatctgga tgcaccggag cgtctgaatc cgcaatttct ggaggacttc
      361 aaactggtgc atcataatga gccgtccttg atgtccacgg gcgttttgtt gtgcggcgtg
      421 gagcgtcgcg gtctggttca cgcgctgcgc gataagtgcc gcagccaagg cattgctatt
      481 cgtttcgaaa gcccgttgct ggaacacggt gagctgccgc tggcggacta tgatctggtg
      541 gtcctggcta atggtgttaa tcacaaaacc gcgcatttca ccgaggctct ggtcccgcag
      601 gtggactacg gccgcaataa gtacatttgg tatggcacta gccagctgtt cgatcagatg
      661 aatctggttt ttcgtaccca tggtaaagat atctttatcg cgcatgccta taagtatagc
      721 gataccatga gcacgttcat tgtcgaatgt agcgaagaga cttacgcacg cgcacgcctg
      781 ggcgaaatgt ccgaagaggc gagcgcagaa tacgttgcta aggtgttcca ggccgagctg
      841 ggtggtcacg gcctggtgag ccagccgggt ctgggttggc gtaacttcat gacgttgtct
      901 catgaccgtt gtcatgatgg taagttggtt ctgctgggtg acgcgctgca aagcggtcac
      961 tttagcatcg gccacggcac cacgatggcc gtggtggtgg cgcagctgct ggttaaagcg
     1021 ctgtgtaccg aagatggtgt gcctgccgcg ctgaaacgtt tcgaagagcg tgccctgccg
     1081 ctggtgcagt tgttccgtgg ccacgcagac aacagccgcg tttggttcga aaccgtcgaa
     1141 gagcgcatgc acctgtcctc ggcggaattt gtgcaaagct tcgacgcacg ccgcaaaagc
     1201 ctgccgccga tgccggaagc actggcgcag aatctgcgtt atgctttgca gcgctgatga
     1261 tcattaagga ggtaaaaaaa atgaaacgtg cgattatcgt tggtggcggc ctggcgggtg
     1321 gcctgaccgc gatctacctg gcgaagcgtg gctacgaagt gcacgtcgtg gagaagcgtg
     1381 gtgatcctct gcgcgatctg agctcttacg tggacgttgt tagcagccgt gcgatcggcg
     1441 tgagcatgac cgttcgtggt atcaagagcg ttttggctgc gggcattccg cgtgcagagc
     1501 tggatgcgtg tggcgaaccg atcgtggcaa tggctttctc cgtgggtggt cagtatcgca
     1561 tgcgcgaact gaagccgttg gaggatttcc gtccgctgag cttgaaccgt gcggcgtttc
     1621 aaaagctgct gaacaaatac gcgaacctgg caggcgttcg ttactacttt gagcataagt
     1681 gcctggatgt tgacctggat ggtaagagcg tgttgattca gggcaaagat ggtcagccgc
     1741 agcgtctgca aggtgacatg attatcggtg cggatggcgc ccacagcgcc gtccgtcagg
     1801 cgatgcagag cggcctgcgt cgtttcgagt tccagcaaac gttcttccgc catggctaca
     1861 aaaccctggt tttgccggac gcgcaagcac tgggttaccg taaagacacg ctgtactttt
     1921 tcggcatgga ttccggtggc ctgttcgcgg gtcgtgcggc tacgatccca gatggtagcg
     1981 tcagcatcgc cgtttgcctg ccgtactcgg gtagcccttc cctgacgacc accgacgaac
     2041 cgacgatgcg tgcgttcttc gatcgttact tcggtggcct gccgcgtgac gcgcgtgacg
     2101 aaatgctgcg tcagtttctg gcgaagccga gcaacgacct gattaacgtg cgctctagca
     2161 cctttcacta taagggtaat gtgctgttgc tgggtgatgc tgcgcatgcg actgcgccgt
     2221 tcctgggtca gggtatgaac atggcgctgg aggacgcccg cacgtttgtc gagctgctgg
     2281 accgccacca gggcgaccaa gacaaagcct ttccggagtt cacggagctg cgcaaagtcc
     2341 aggcagacgc aatgcaagac atggctcgcg ccaactatga cgttttgagc tgctcgaacc
     2401 cgatcttttt catgcgtgcg cgttacacgc gttacatgca ttccaagttt ccgggcctgt
     2461 atccgccgga tatggccgag aaactgtact ttacgagcga gccgtacgat cgtctgcaac
     2521 aaatccagcg taaacagaat gtttggtaca agattggtcg cgtgaattga agatct
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