BBa_K726017 1 BBa_K726017 VioDC 2012-10-01T11:00:00Z 2015-05-08T01:13:05Z The original source is Chromobacterium violaceum. This contains the coding sequence for the last two enzymes in the violacein production pathway. There is a ribosome binding site between the two genes. false false _972_ 0 10295 9 Not in stock false n/a false Veronica Zepeda annotation2206724 1 VioD range2206724 1 1 1129 annotation2206797 1 RBS range2206797 1 1129 1146 annotation2206799 1 VioC range2206799 1 1147 2441 BBa_K726018 1 BBa_K726018 T7Prom+HisTag+VioDC 2012-10-01T11:00:00Z 2015-05-08T01:13:05Z The DNA is from Chrombacterium violaceum and a Novagen pCDFDuet vector. This part contains a T7 promoter, lac operator, 6x his tag and the coding sequence for VioD and VioC (enzymes in the violacein production pathway). false false _972_ 0 10295 9 It's complicated false n/a false Veronica Zepeda component2206835 1 BBa_K726003 component2206839 1 BBa_K726017 annotation2206839 1 BBa_K726017 range2206839 1 136 2576 annotation2206835 1 BBa_K726003 range2206835 1 1 129 BBa_K726003 1 BBa_K726003 T7 promoter + lac operator + His tag 2012-08-08T11:00:00Z 2015-05-08T01:13:05Z The sequence is from pCDFDuet from Novagen. T7 promoter is good for protein expression in <i>e. coli</i>. Requires the T7 RNA polymerase. Also contains a RBS, and a His tag for protein purification. Insert your protein of interest behind this. false false _972_ 0 10304 9 Not in stock false none. false Sami Chu annotation2179384 1 T7 promoter range2179384 1 1 17 annotation2179385 1 His tag range2179385 1 100 117 annotation2179473 1 lac operator range2179473 1 22 42 BBa_K726018_sequence 1 taatacgactcactataggggaattgtgagcggataacaattcccctgtagaaataattttgtttaactttaataaggagatataccatgggcagcagccatcaccatcatcaccacagccaggatccgtactagatgaagattctggtcattggtgctggtccagctggtctggttttcgcatcccaactgaagcaggcacgccctttgtgggccattgacatcgtggagaagaatgacgagcaagaagtgctgggctggggtgtcgtgctgcctggccgtccgggtcagcacccggcgaacccgctgtcctatctggatgcaccggagcgtctgaatccgcaatttctggaggacttcaaactggtgcatcataatgagccgtccttgatgtccacgggcgttttgttgtgcggcgtggagcgtcgcggtctggttcacgcgctgcgcgataagtgccgcagccaaggcattgctattcgtttcgaaagcccgttgctggaacacggtgagctgccgctggcggactatgatctggtggtcctggctaatggtgttaatcacaaaaccgcgcatttcaccgaggctctggtcccgcaggtggactacggccgcaataagtacatttggtatggcactagccagctgttcgatcagatgaatctggtttttcgtacccatggtaaagatatctttatcgcgcatgcctataagtatagcgataccatgagcacgttcattgtcgaatgtagcgaagagacttacgcacgcgcacgcctgggcgaaatgtccgaagaggcgagcgcagaatacgttgctaaggtgttccaggccgagctgggtggtcacggcctggtgagccagccgggtctgggttggcgtaacttcatgacgttgtctcatgaccgttgtcatgatggtaagttggttctgctgggtgacgcgctgcaaagcggtcactttagcatcggccacggcaccacgatggccgtggtggtggcgcagctgctggttaaagcgctgtgtaccgaagatggtgtgcctgccgcgctgaaacgtttcgaagagcgtgccctgccgctggtgcagttgttccgtggccacgcagacaacagccgcgtttggttcgaaaccgtcgaagagcgcatgcacctgtcctcggcggaatttgtgcaaagcttcgacgcacgccgcaaaagcctgccgccgatgccggaagcactggcgcagaatctgcgttatgctttgcagcgctgatgatcattaaggaggtaaaaaaaatgaaacgtgcgattatcgttggtggcggcctggcgggtggcctgaccgcgatctacctggcgaagcgtggctacgaagtgcacgtcgtggagaagcgtggtgatcctctgcgcgatctgagctcttacgtggacgttgttagcagccgtgcgatcggcgtgagcatgaccgttcgtggtatcaagagcgttttggctgcgggcattccgcgtgcagagctggatgcgtgtggcgaaccgatcgtggcaatggctttctccgtgggtggtcagtatcgcatgcgcgaactgaagccgttggaggatttccgtccgctgagcttgaaccgtgcggcgtttcaaaagctgctgaacaaatacgcgaacctggcaggcgttcgttactactttgagcataagtgcctggatgttgacctggatggtaagagcgtgttgattcagggcaaagatggtcagccgcagcgtctgcaaggtgacatgattatcggtgcggatggcgcccacagcgccgtccgtcaggcgatgcagagcggcctgcgtcgtttcgagttccagcaaacgttcttccgccatggctacaaaaccctggttttgccggacgcgcaagcactgggttaccgtaaagacacgctgtactttttcggcatggattccggtggcctgttcgcgggtcgtgcggctacgatcccagatggtagcgtcagcatcgccgtttgcctgccgtactcgggtagcccttccctgacgaccaccgacgaaccgacgatgcgtgcgttcttcgatcgttacttcggtggcctgccgcgtgacgcgcgtgacgaaatgctgcgtcagtttctggcgaagccgagcaacgacctgattaacgtgcgctctagcacctttcactataagggtaatgtgctgttgctgggtgatgctgcgcatgcgactgcgccgttcctgggtcagggtatgaacatggcgctggaggacgcccgcacgtttgtcgagctgctggaccgccaccagggcgaccaagacaaagcctttccggagttcacggagctgcgcaaagtccaggcagacgcaatgcaagacatggctcgcgccaactatgacgttttgagctgctcgaacccgatctttttcatgcgtgcgcgttacacgcgttacatgcattccaagtttccgggcctgtatccgccggatatggccgagaaactgtactttacgagcgagccgtacgatcgtctgcaacaaatccagcgtaaacagaatgtttggtacaagattggtcgcgtgaattgaagatct BBa_K726017_sequence 1 atgaagattctggtcattggtgctggtccagctggtctggttttcgcatcccaactgaagcaggcacgccctttgtgggccattgacatcgtggagaagaatgacgagcaagaagtgctgggctggggtgtcgtgctgcctggccgtccgggtcagcacccggcgaacccgctgtcctatctggatgcaccggagcgtctgaatccgcaatttctggaggacttcaaactggtgcatcataatgagccgtccttgatgtccacgggcgttttgttgtgcggcgtggagcgtcgcggtctggttcacgcgctgcgcgataagtgccgcagccaaggcattgctattcgtttcgaaagcccgttgctggaacacggtgagctgccgctggcggactatgatctggtggtcctggctaatggtgttaatcacaaaaccgcgcatttcaccgaggctctggtcccgcaggtggactacggccgcaataagtacatttggtatggcactagccagctgttcgatcagatgaatctggtttttcgtacccatggtaaagatatctttatcgcgcatgcctataagtatagcgataccatgagcacgttcattgtcgaatgtagcgaagagacttacgcacgcgcacgcctgggcgaaatgtccgaagaggcgagcgcagaatacgttgctaaggtgttccaggccgagctgggtggtcacggcctggtgagccagccgggtctgggttggcgtaacttcatgacgttgtctcatgaccgttgtcatgatggtaagttggttctgctgggtgacgcgctgcaaagcggtcactttagcatcggccacggcaccacgatggccgtggtggtggcgcagctgctggttaaagcgctgtgtaccgaagatggtgtgcctgccgcgctgaaacgtttcgaagagcgtgccctgccgctggtgcagttgttccgtggccacgcagacaacagccgcgtttggttcgaaaccgtcgaagagcgcatgcacctgtcctcggcggaatttgtgcaaagcttcgacgcacgccgcaaaagcctgccgccgatgccggaagcactggcgcagaatctgcgttatgctttgcagcgctgatgatcattaaggaggtaaaaaaaatgaaacgtgcgattatcgttggtggcggcctggcgggtggcctgaccgcgatctacctggcgaagcgtggctacgaagtgcacgtcgtggagaagcgtggtgatcctctgcgcgatctgagctcttacgtggacgttgttagcagccgtgcgatcggcgtgagcatgaccgttcgtggtatcaagagcgttttggctgcgggcattccgcgtgcagagctggatgcgtgtggcgaaccgatcgtggcaatggctttctccgtgggtggtcagtatcgcatgcgcgaactgaagccgttggaggatttccgtccgctgagcttgaaccgtgcggcgtttcaaaagctgctgaacaaatacgcgaacctggcaggcgttcgttactactttgagcataagtgcctggatgttgacctggatggtaagagcgtgttgattcagggcaaagatggtcagccgcagcgtctgcaaggtgacatgattatcggtgcggatggcgcccacagcgccgtccgtcaggcgatgcagagcggcctgcgtcgtttcgagttccagcaaacgttcttccgccatggctacaaaaccctggttttgccggacgcgcaagcactgggttaccgtaaagacacgctgtactttttcggcatggattccggtggcctgttcgcgggtcgtgcggctacgatcccagatggtagcgtcagcatcgccgtttgcctgccgtactcgggtagcccttccctgacgaccaccgacgaaccgacgatgcgtgcgttcttcgatcgttacttcggtggcctgccgcgtgacgcgcgtgacgaaatgctgcgtcagtttctggcgaagccgagcaacgacctgattaacgtgcgctctagcacctttcactataagggtaatgtgctgttgctgggtgatgctgcgcatgcgactgcgccgttcctgggtcagggtatgaacatggcgctggaggacgcccgcacgtttgtcgagctgctggaccgccaccagggcgaccaagacaaagcctttccggagttcacggagctgcgcaaagtccaggcagacgcaatgcaagacatggctcgcgccaactatgacgttttgagctgctcgaacccgatctttttcatgcgtgcgcgttacacgcgttacatgcattccaagtttccgggcctgtatccgccggatatggccgagaaactgtactttacgagcgagccgtacgatcgtctgcaacaaatccagcgtaaacagaatgtttggtacaagattggtcgcgtgaattgaagatct BBa_K726003_sequence 1 taatacgactcactataggggaattgtgagcggataacaattcccctgtagaaataattttgtttaactttaataaggagatataccatgggcagcagccatcaccatcatcaccacagccaggatccg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z