BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0062
1
lux pR
Promoter (luxR & HSL regulated -- lux pR)
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
<em>V. fischeri</em>
Released HQ 2013
Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
false
true
_1_
0
24
7
In stock
false
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2045
1
LuxR/HSL
range2045
1
1
20
annotation2047
1
-10
range2047
1
42
47
annotation2046
1
-35
range2046
1
20
25
annotation2048
1
start
range2048
1
53
53
annotation7070
1
BBa_R0062
range7070
1
1
55
BBa_C0062
1
luxr
luxR repressor/activator, (no LVA?)
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
<em>V. fischeri</em> <genbank>AF170104</genbank>
Released HQ 2013
In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
false
true
_1_
0
24
7
In stock
false
<P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation7039
1
BBa_C0062
range7039
1
1
756
annotation1762
1
prefix
range1762
1
1
2
annotation1766
1
luxR
range1766
1
1
750
annotation1764
1
T
range1764
1
174
174
annotation1765
1
A
range1765
1
492
492
annotation2213986
1
Help:Barcodes
range2213986
1
757
781
BBa_I0462
1
LuxR
luxR Protein Generator
2003-12-04T12:00:00Z
2015-08-31T04:07:29Z
Released HQ 2013
Produces LuxR protein which can sense 3OC<sub>6</sub>HSL in the media and activate transcription from R0062, the right hand Lux promoter
false
false
_1_
0
24
7
In stock
false
true
Caitlin Conboy and Jennifer Braff
component943199
1
BBa_B0012
component943181
1
BBa_C0062
component943189
1
BBa_B0010
component943166
1
BBa_B0034
annotation943189
1
BBa_B0010
range943189
1
808
887
annotation943181
1
BBa_C0062
range943181
1
19
774
annotation943166
1
BBa_B0034
range943166
1
1
12
annotation943199
1
BBa_B0012
range943199
1
896
936
BBa_K727010
1
BBa_K727010
Rose Odor Generator - Component One
2012-06-15T11:00:00Z
2015-05-08T01:13:05Z
Genome of
GPPS (Geranyl diphosphate synthase) synthesizes GPP (Geranyl diphosphate) which is the first reaction of geraniol production. The promoter is attached into the part with the aim of easier regulation.
Geraniol is a monoterpenoid and an alcohol. It???s molecular formula is C10H18O . It is the primary part of rose oil, palmarosa oil, and citronella oil (Java type). It also occurs in small quantities in geranium, lemon, and many other essential oils.
It has a rose-like odor and is commonly used in perfumes. It is used in flavors such as peach, raspberry, grapefruit, red apple, plum, lime, orange, lemon, watermelon, pineapple, and blueberry.
false
false
_973_
0
11153
9
Not in stock
true
Optimized for E.coli
false
İBRAHİM YASİR ORHAN
component2269054
1
BBa_K727005
component2269052
1
BBa_J09855
annotation2269054
1
BBa_K727005
range2269054
1
1053
1993
annotation2269052
1
BBa_J09855
range2269052
1
1
1044
BBa_K727005
1
BBa_K727005
Geranyl diphosphate Synthase
2012-06-15T11:00:00Z
2015-05-08T01:13:05Z
Genom on
GPPS (Geranyl diphosphate synthase) synthesizes GPP (Geranyl diphosphate) which is the first reaction of geraniol production.
false
false
_973_
0
11153
9
Not in stock
true
Optemized for E.coli
false
İBRAHİM YASİR ORHAN
annotation2176259
1
GPPS
range2176259
1
1
941
BBa_I14032
1
P(Lac) IQ
promoter P(Lac) IQ
2004-08-03T11:00:00Z
2015-08-31T04:07:37Z
Plasmid pMAL-p2X
Released HQ 2013
Constitutive Promoter, High Transcription
false
true
_4_
0
171
7
In stock
false
true
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
annotation1028344
1
-10
range1028344
1
26
31
annotation1028342
1
P(Lac) IQ
range1028342
1
1
37
annotation1028343
1
-35
range1028343
1
3
8
BBa_J09855
1
BBa_J09855
Constitutive luxR device with pLuxR
2006-10-25T11:00:00Z
2015-08-31T04:08:21Z
This part was constructed from registry biobricks.
Released HQ 2013
I14032.I0462.R0062
false
true
_72_40_
0
475
72
In stock
true
This is a universal part designed to allow induction by HSL of whatever is assembled downstream of the part.
true
Jon Badalamenti
component2260447
1
BBa_R0062
component2260430
1
BBa_I14032
component2260445
1
BBa_I0462
annotation2260430
1
BBa_I14032
range2260430
1
1
37
annotation2260445
1
BBa_I0462
range2260445
1
46
981
annotation2260447
1
BBa_R0062
range2260447
1
990
1044
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0062_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J09855_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
BBa_I14032_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcc
BBa_I0462_sequence
1
aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K727010_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagaggaattcgcggccgcttctagatggattttccgcagcagctggaagcatgtgttaaacaggcaaatcaggcactgagccgttttattgcaccgctgccgtttcagaataccccggttgttgaaaccatgcagtatggcgcactgctgggtggtaaacgtctgcgtccgtttctggtttatgcaaccggtcatatgtttggtgttagcaccaataccctggatgcaccggcagcagcagttgaatgtattcatgcatatagcctgattcatgatgatctgccggcaatggatgatgatgatctgcgtcgtggtctgccgacctgtcatgttaaatttggcgaagcaaatgcaattctggcaggtgatgcacttcagaccctggcatttagcattctgagcgatgcagatatgccggaagttagcgatcgtgatcgtattagcatgattagcgaactggcaagcgcaagcggtattgcaggtatgtgtggtggtcaggcactggatctggatgcagaaggtaaacatgttccgctggatgcactggaacgtattcatcgtcataaaaccggtgcactgattcgtgcagcagttcgtctgggtgcactgagcgcaggtgataaaggtcgtcgtgcactgccggttctggataaatatgcagaaagcattggtctggcatttcaggttcaggatgatattctggatgttgttggtgataccgcaaccctgggtaaacgtcagggtgcagatcagcagctgggtaaaagcacctatccggcactgctgggtctggaacaggcacgtaaaaaagcacaggatctgattgatgatgcacgtcagagcctgaaacagctggcagaacagagcattgataccagcgcactggaagcactggcagattatattattcagcgtaataaataatactagtagcggccgctgcag
BBa_K727005_sequence
1
gaattcgcggccgcttctagatggattttccgcagcagctggaagcatgtgttaaacaggcaaatcaggcactgagccgttttattgcaccgctgccgtttcagaataccccggttgttgaaaccatgcagtatggcgcactgctgggtggtaaacgtctgcgtccgtttctggtttatgcaaccggtcatatgtttggtgttagcaccaataccctggatgcaccggcagcagcagttgaatgtattcatgcatatagcctgattcatgatgatctgccggcaatggatgatgatgatctgcgtcgtggtctgccgacctgtcatgttaaatttggcgaagcaaatgcaattctggcaggtgatgcacttcagaccctggcatttagcattctgagcgatgcagatatgccggaagttagcgatcgtgatcgtattagcatgattagcgaactggcaagcgcaagcggtattgcaggtatgtgtggtggtcaggcactggatctggatgcagaaggtaaacatgttccgctggatgcactggaacgtattcatcgtcataaaaccggtgcactgattcgtgcagcagttcgtctgggtgcactgagcgcaggtgataaaggtcgtcgtgcactgccggttctggataaatatgcagaaagcattggtctggcatttcaggttcaggatgatattctggatgttgttggtgataccgcaaccctgggtaaacgtcagggtgcagatcagcagctgggtaaaagcacctatccggcactgctgggtctggaacaggcacgtaaaaaagcacaggatctgattgatgatgcacgtcagagcctgaaacagctggcagaacagagcattgataccagcgcactggaagcactggcagattatattattcagcgtaataaataatactagtagcggccgctgcag
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_C0062_sequence
1
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z