BBa_K727107
1
BBa_K727107
GPPS coding device with GFP
2012-06-24T11:00:00Z
2015-05-08T01:13:05Z
Genome of
GPPS (Geranyl diphosphate synthase) synthesizes GPP (Geranyl diphosphate) which is the first reaction of geraniol production. The promoter is attached into the part with the aim of easier regulation.
GFP region is added with aim to control whether the enzyme is synthesized. The green colonies will show that GFP is produced with GPPS.
false
false
_973_
0
9573
9
Not in stock
true
Optimized for E.coli
false
Mustafa Elitok
component2177186
1
BBa_E0040
component2177188
1
BBa_K727005
component2177184
1
BBa_J04500
annotation2177184
1
BBa_J04500
range2177184
1
1
220
annotation2177188
1
BBa_K727005
range2177188
1
955
1895
annotation2177186
1
BBa_E0040
range2177186
1
227
946
BBa_K727005
1
BBa_K727005
Geranyl diphosphate Synthase
2012-06-15T11:00:00Z
2015-05-08T01:13:05Z
Genom on
GPPS (Geranyl diphosphate synthase) synthesizes GPP (Geranyl diphosphate) which is the first reaction of geraniol production.
false
false
_973_
0
11153
9
Not in stock
true
Optemized for E.coli
false
İBRAHİM YASİR ORHAN
annotation2176259
1
GPPS
range2176259
1
1
941
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J04500
1
BBa_J04500
IPTG inducible promoter with RBS
2005-06-08T11:00:00Z
2015-08-31T04:08:14Z
Davidson Synth-Aces
Released HQ 2013
R0010.B0034
false
true
_16_
0
326
16
In stock
false
false
Kristen DeCelle
component1508159
1
BBa_B0034
component1508149
1
BBa_R0010
annotation1508159
1
BBa_B0034
range1508159
1
209
220
annotation1508149
1
BBa_R0010
range1508149
1
1
200
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961224
1
-35
range1961224
1
137
142
annotation1961227
1
start
range1961227
1
173
173
annotation1961225
1
-10
range1961225
1
161
166
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K727107_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagaggaattcgcggccgcttctagatggattttccgcagcagctggaagcatgtgttaaacaggcaaatcaggcactgagccgttttattgcaccgctgccgtttcagaataccccggttgttgaaaccatgcagtatggcgcactgctgggtggtaaacgtctgcgtccgtttctggtttatgcaaccggtcatatgtttggtgttagcaccaataccctggatgcaccggcagcagcagttgaatgtattcatgcatatagcctgattcatgatgatctgccggcaatggatgatgatgatctgcgtcgtggtctgccgacctgtcatgttaaatttggcgaagcaaatgcaattctggcaggtgatgcacttcagaccctggcatttagcattctgagcgatgcagatatgccggaagttagcgatcgtgatcgtattagcatgattagcgaactggcaagcgcaagcggtattgcaggtatgtgtggtggtcaggcactggatctggatgcagaaggtaaacatgttccgctggatgcactggaacgtattcatcgtcataaaaccggtgcactgattcgtgcagcagttcgtctgggtgcactgagcgcaggtgataaaggtcgtcgtgcactgccggttctggataaatatgcagaaagcattggtctggcatttcaggttcaggatgatattctggatgttgttggtgataccgcaaccctgggtaaacgtcagggtgcagatcagcagctgggtaaaagcacctatccggcactgctgggtctggaacaggcacgtaaaaaagcacaggatctgattgatgatgcacgtcagagcctgaaacagctggcagaacagagcattgataccagcgcactggaagcactggcagattatattattcagcgtaataaataatactagtagcggccgctgcag
BBa_J04500_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
BBa_K727005_sequence
1
gaattcgcggccgcttctagatggattttccgcagcagctggaagcatgtgttaaacaggcaaatcaggcactgagccgttttattgcaccgctgccgtttcagaataccccggttgttgaaaccatgcagtatggcgcactgctgggtggtaaacgtctgcgtccgtttctggtttatgcaaccggtcatatgtttggtgttagcaccaataccctggatgcaccggcagcagcagttgaatgtattcatgcatatagcctgattcatgatgatctgccggcaatggatgatgatgatctgcgtcgtggtctgccgacctgtcatgttaaatttggcgaagcaaatgcaattctggcaggtgatgcacttcagaccctggcatttagcattctgagcgatgcagatatgccggaagttagcgatcgtgatcgtattagcatgattagcgaactggcaagcgcaagcggtattgcaggtatgtgtggtggtcaggcactggatctggatgcagaaggtaaacatgttccgctggatgcactggaacgtattcatcgtcataaaaccggtgcactgattcgtgcagcagttcgtctgggtgcactgagcgcaggtgataaaggtcgtcgtgcactgccggttctggataaatatgcagaaagcattggtctggcatttcaggttcaggatgatattctggatgttgttggtgataccgcaaccctgggtaaacgtcagggtgcagatcagcagctgggtaaaagcacctatccggcactgctgggtctggaacaggcacgtaaaaaagcacaggatctgattgatgatgcacgtcagagcctgaaacagctggcagaacagagcattgataccagcgcactggaagcactggcagattatattattcagcgtaataaataatactagtagcggccgctgcag
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z