BBa_R0063
1
lux pL
Promoter (luxR & HSL regulated -- lux pL)<br>
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
<em>V. fischeri.</em>
Released HQ 2013
The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl</p>
false
true
_1_
0
24
7
In stock
false
<P> <P> This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <p><img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"><P>Unspecified.
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2053
1
-35
range2053
1
89
94
annotation2055
1
Putative LuxR/HSL
range2055
1
130
149
annotation7071
1
BBa_R0063
range7071
1
1
151
annotation2054
1
start
range2054
1
128
128
annotation2052
1
-10
range2052
1
115
122
annotation2051
1
LuxR/HSL
range2051
1
1
20
BBa_K727272
1
BBa_K727272
Fish Odor Generator-Complete System
2012-06-15T11:00:00Z
2015-05-08T01:13:05Z
Genom of
TMT (trimethylamine transferase) takes part in the third reaction of the production of TMA (trimethylamine) which is the major cause of unpleasant fish odor. We added Lux cassette right promoter that up-regulated by HSL and LuxR in the course of being able to regulate easily. We intended to generate a negative feedback response with the help of producing LuxR and HSL and activating the promoter with them.
false
false
_973_
0
11153
9
Not in stock
false
Optimized for E.coli
false
İBRAHİM YASİR ORHAN
component2264452
1
BBa_K727003
component2264448
1
BBa_K727001
component2264450
1
BBa_K727002
component2264460
1
BBa_C0061
component2264455
1
BBa_C0062
component2264442
1
BBa_R0063
annotation2264448
1
BBa_K727001
range2264448
1
160
1574
annotation2264442
1
BBa_R0063
range2264442
1
1
151
annotation2264450
1
BBa_K727002
range2264450
1
1583
3024
annotation2264460
1
BBa_C0061
range2264460
1
5349
5966
annotation2264452
1
BBa_K727003
range2264452
1
3033
4555
annotation2264455
1
BBa_C0062
range2264455
1
4562
5317
BBa_C0061
1
luxI
autoinducer synthetase for AHL
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
<em>V. fischeri</em> <genbank>AF170104</genbank>
Released HQ 2013
Synthesizes 3OC<sub>6</sub>HSL, which binds to LuxR.</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. . <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation1760
1
LVA
range1760
1
580
611
annotation2213985
1
Help:Barcodes
range2213985
1
619
643
annotation7038
1
BBa_C0061
range7038
1
1
618
annotation1761
1
luxI
range1761
1
1
579
BBa_K727002
1
BBa_K727002
Dimethylamine Methyltransferase (DMT)
2012-06-15T11:00:00Z
2015-05-08T01:13:05Z
Genome of
DMT (monomethylamine transferase) catalyses the second reaction of TMA (trimethylamine) synthesis. TMA is the major cause of unpleasant fish odor.
false
false
_973_
0
9573
9
Not in stock
true
Optimized for E.coli
false
Mustafa Elitok
annotation2176576
1
DMT
range2176576
1
1
1442
BBa_K727003
1
BBa_K727003
Trimethylamine Methyltransferase (TMT)
2012-06-15T11:00:00Z
2015-05-08T01:13:05Z
Genome of
TMT (trimethylamine transferase) takes part in the third reaction of the production of TMA (trimethylamine) which is the major cause of unpleasant fish odor.
false
false
_973_
0
11153
9
Not in stock
true
Optimized for E.coli
false
İBRAHİM YASİR ORHAN
annotation2176257
1
TMT
range2176257
1
1
1523
BBa_C0062
1
luxr
luxR repressor/activator, (no LVA?)
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
<em>V. fischeri</em> <genbank>AF170104</genbank>
Released HQ 2013
In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
false
true
_1_
0
24
7
In stock
false
<P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2213986
1
Help:Barcodes
range2213986
1
757
781
annotation1762
1
prefix
range1762
1
1
2
annotation1766
1
luxR
range1766
1
1
750
annotation1764
1
T
range1764
1
174
174
annotation7039
1
BBa_C0062
range7039
1
1
756
annotation1765
1
A
range1765
1
492
492
BBa_K727001
1
BBa_K727001
Monomethylamine Methyltransferase (MMT)
2012-06-15T11:00:00Z
2015-05-08T01:13:05Z
Genome of
MMT (monomethylamine transferase) catalyses the first reaction of TMA (trimethylamine) synthesis. TMA is the major cause of unpleasant fish odor.
For additional information: http://2012hs.igem.org/Team:AUC_Turkey/Project/TMA
false
false
_973_
0
9573
9
Not in stock
true
Optimized for E.coli
false
Mustafa Elitok
annotation2176577
1
MMT
range2176577
1
1
1415
annotation2176578
1
BBa_K727001
range2176578
1
1
1415
BBa_R0063_sequence
1
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaa
BBa_C0061_sequence
1
atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
BBa_K727001_sequence
1
gaattcgcggccgcttctagatgatgacctttcgtaaatattttgattgttatgatttttatgatcgtgcaaaagttggtgaaaaatgtaccctggatgattgggatctgatgaaaattccgatgaaagcaatggaactgaaacagaaatatggtctggattttaaaggtgaatttattccgaccgataaagatatgatggaaaaactgtttaaagcaggttttgaaatgctgctggaatgtggtatttattgtaccgatacccatcgtattgttaaatataccgaagatgaaatttgggatgcaattaataatgttcagaaagaatttgttctgggtaccggtcgtgatgcagttaatgttcgtaaacgtagcgttggtgataaagcaaaaccgattgttcagggtggtccgaccggtagcccgattagcgaagatgtttttatgccggttcatatgagctatgcactggaaaaagaagttgataccattgttaatggtgttatgaccaccgttcgtggtaaagcaccgattccgaaaagcccgtatgaagttctggcagcaaaaaccgaaacccgtctgattaaaaatgcatgtgcaatggcaggtcgtccgggtatgggtgttggtccggaaaccagcctgagcgcacagggtaatattagcgcagattgtgcaggtggtatgacctgtaccgatagccatgaagttagccagctgtgtgaactgaaaattgatctggatgcaattagcgttattgcacattataccgcaaatagcgatattattatggatgaacagatgccgatttttggtggttatgcaggtggtattgaagaaaccaccattgttgatgttgcaacccatattaatgcagttctgatgagcagcgcaagctggcatctggatggtccggttcatattcgttggggtagcaccaatacccgtgaaaccctgatgattgcaggttgggcatgtgcaaccattagcgaatttaccgatattctgagcggtaatcagtattatccgtgtgcaggtccgtgtaccgaaatgtgtctgctggaagcaagcgcacagagcattaccgataccgcaagcggtcgtgaaattctgagcggtgttgcaagcgcaaaaggtgttgttaccgataaaaccaccggtatggaagcacgtatgatgggtgaagttgcacgtgcaaccgcaggtgttgaaattagcgaagttaatgttattctggataaactggttgcactgtatgaaaaaaattatgcaagcgcaccggcaggtaaaacctttcaggaatgttatgatgttaaaaccgttaccccgaccgatgaatatatgcaggtttatgatggtgcacgtaaaaaactggaagatctgggtctggttttttactagtagcggccgctgcag
BBa_K727003_sequence
1
gaattcgcggccgcttctagatggcaaaaaataatgcagttgcaggttttaatgcactgaatggtgttgaactgaatctgtttaccaccgatgaactgaaagcaattcattatgcaaccatggaagttctgatggatccgggtattcaggttagcgatccggaagcacgtcagatttttaaagaaaatggttgtgaagttaatgaaaaaaccaatgttgttaaaattccggaatatctggttcgtaaagcattacagctggcaccgagccgttttgttctgtggggtcgtgataaaaaatttaataccgttcaggaatgtggtggtaaagttcattggacctgttttggtaccggtgttaaagtttgtaaatatcaggatggtaaatatgttaccgttgatagcgttgaaaaagatattgcagatattgcaaaactgtgtgattgggcagaaaatattgattattttagcctgccggttagcgcacgtgatattgcaggtcagggtgcacaggatgttcatgaaaccctgaccccgctggcaaataccgcaaaacattttcatcatattgatccggttggtgaaaatgttgaatattatcgtgatattgttaaagcatattatggtggtgatgaagaagaagcacgtaaaaaaccgatttttagcatgctgctgtgtccgaccagcccgctggaactgagcgttaatgcatgtcaggttattattaaaggtgcacgttttggtattccggttaatgttctgagcatggcaatgagcggtggtagcagcccggtttatctggcaggtaccctggttacccataatgcagaagttctgagcggtattgttctggcacagctgaccgttccgggtgcaaaagtttggtatggtagcagcaccaccacctttgatctgaaaaaaggtaccgcaccggttggtagcccggaactgggtctgattagcgcagcagttgcaaaactggcacagttttatggtctgccgagctatgttgcaggtagcagcgatgcaaaagttccggatgatcaggcaggtcatgaaaaaaccatgaccaccctgctgccggcactggcaggtgcaaataccatttatggtgcaggtatgctggaactgggtatgacctttagcatggaacagctggttattgataatgatatttttagcatggttaaaaaagcaatgcagggtattccggttagcgaagaaaccctggcagttgaaagcattcagaaagttggtattggtaataattttctggcactgaaacagacccgtcagctggttgattatccgagcaatccgatgctgctggatcgtcatatgtttggtgattgggcagcagcaggtagcaaagatctggcaaccgttgcacatgaaaaagttgaagatgttctgaaaaatcatcaggttaccccgattgatgcagatatttttaaagatatgcaggcaattgttgataaagcagataaagcatttcgtggtatgtactagtagcggccgctgcag
BBa_K727272_sequence
1
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagaggaattcgcggccgcttctagatgatgacctttcgtaaatattttgattgttatgatttttatgatcgtgcaaaagttggtgaaaaatgtaccctggatgattgggatctgatgaaaattccgatgaaagcaatggaactgaaacagaaatatggtctggattttaaaggtgaatttattccgaccgataaagatatgatggaaaaactgtttaaagcaggttttgaaatgctgctggaatgtggtatttattgtaccgatacccatcgtattgttaaatataccgaagatgaaatttgggatgcaattaataatgttcagaaagaatttgttctgggtaccggtcgtgatgcagttaatgttcgtaaacgtagcgttggtgataaagcaaaaccgattgttcagggtggtccgaccggtagcccgattagcgaagatgtttttatgccggttcatatgagctatgcactggaaaaagaagttgataccattgttaatggtgttatgaccaccgttcgtggtaaagcaccgattccgaaaagcccgtatgaagttctggcagcaaaaaccgaaacccgtctgattaaaaatgcatgtgcaatggcaggtcgtccgggtatgggtgttggtccggaaaccagcctgagcgcacagggtaatattagcgcagattgtgcaggtggtatgacctgtaccgatagccatgaagttagccagctgtgtgaactgaaaattgatctggatgcaattagcgttattgcacattataccgcaaatagcgatattattatggatgaacagatgccgatttttggtggttatgcaggtggtattgaagaaaccaccattgttgatgttgcaacccatattaatgcagttctgatgagcagcgcaagctggcatctggatggtccggttcatattcgttggggtagcaccaatacccgtgaaaccctgatgattgcaggttgggcatgtgcaaccattagcgaatttaccgatattctgagcggtaatcagtattatccgtgtgcaggtccgtgtaccgaaatgtgtctgctggaagcaagcgcacagagcattaccgataccgcaagcggtcgtgaaattctgagcggtgttgcaagcgcaaaaggtgttgttaccgataaaaccaccggtatggaagcacgtatgatgggtgaagttgcacgtgcaaccgcaggtgttgaaattagcgaagttaatgttattctggataaactggttgcactgtatgaaaaaaattatgcaagcgcaccggcaggtaaaacctttcaggaatgttatgatgttaaaaccgttaccccgaccgatgaatatatgcaggtttatgatggtgcacgtaaaaaactggaagatctgggtctggttttttactagtagcggccgctgcagtactagaggaattcgcggccgcttctagatgatggcaaccgaatatgcactgcgtatgggtgatggtaaacgtatttatctgaccaaagaaaaaattctgagcgaaattgaagcaggtagcagcaatgcagcagatctgggtgatattccggatctgagcaccgatgaaatgaaaaaactggcagaaattctgatgatgccgggtaaagcagttagcgttgaacagggtatggaagttccggttacccatgatattggtaccattcgtctggatggtgatcagggtaatagcggtgttggtattccgagcagccgtctggttggttgtatgacccatgaacgtgcatttggtgcagataccatggaactgggtcatattgattatagctttaaaccggttaaaccggttgttagcaatgaatgtcaggcaatggaagtttgtcagcagaatatgattattccgctgttttatggtgcaatgccgaatatgggtctgtattataccccggatggtccgtttgaaaatccgggtgatctgatgaaaatgtttaaaattgataaagcacgtgaaagcatggaacatgcagcagatcatctgacccgtgataccgtttgggttatgcagaaactgtttgcaagcggtgcagatggtgttaattttgataccaccgcagcagcaggtgatgcagatatgtatggtaccctgcgtgcagttgaagttctgcgtgcacagtttccggaaatgtatattgaagttggtatggcaggtgaaatggttctgggtatgcatggtgaactggaatatgatgaagttcgtctggcaggtctgtggccgcatgaacaggcaccgctgattgcaaaagcaggtgcaaatgtttttggtccggttgttaataccaataccagcaaaaccagcgcatggaatctggcacgtgcagttacctttattaaagaagcagttaaagcaagcccgattccgtgtcatgttaatatgggtatgggtgttggtggtattccgatgctggaaaccccgccggttgatgcagttacccgtgcaagcaaagcaatggttgaagttgcaggtgttgatggtattattggtgttggtgatccgctgggtatgccgattagccatattatggcaagcggtatgaccggtattcgtgcagcaggtgatctggttgcacgtatggaatttagcaaaaatatgcgtattggtgaagcaaaagaatatgttgcaaaaaaactgaatgttgataccatggatctggcagatgaacatgttatgcgtgaactgcgtgaagaactggatattggtgttattaccagcgttccgggtgcagcaaaaggtattgcagcaaaaatgaatattgaaaaactgctgggtattaaaattaatagctgtgaaacctttcgtgcacagctggcatactagtagcggccgctgcagtactagaggaattcgcggccgcttctagatggcaaaaaataatgcagttgcaggttttaatgcactgaatggtgttgaactgaatctgtttaccaccgatgaactgaaagcaattcattatgcaaccatggaagttctgatggatccgggtattcaggttagcgatccggaagcacgtcagatttttaaagaaaatggttgtgaagttaatgaaaaaaccaatgttgttaaaattccggaatatctggttcgtaaagcattacagctggcaccgagccgttttgttctgtggggtcgtgataaaaaatttaataccgttcaggaatgtggtggtaaagttcattggacctgttttggtaccggtgttaaagtttgtaaatatcaggatggtaaatatgttaccgttgatagcgttgaaaaagatattgcagatattgcaaaactgtgtgattgggcagaaaatattgattattttagcctgccggttagcgcacgtgatattgcaggtcagggtgcacaggatgttcatgaaaccctgaccccgctggcaaataccgcaaaacattttcatcatattgatccggttggtgaaaatgttgaatattatcgtgatattgttaaagcatattatggtggtgatgaagaagaagcacgtaaaaaaccgatttttagcatgctgctgtgtccgaccagcccgctggaactgagcgttaatgcatgtcaggttattattaaaggtgcacgttttggtattccggttaatgttctgagcatggcaatgagcggtggtagcagcccggtttatctggcaggtaccctggttacccataatgcagaagttctgagcggtattgttctggcacagctgaccgttccgggtgcaaaagtttggtatggtagcagcaccaccacctttgatctgaaaaaaggtaccgcaccggttggtagcccggaactgggtctgattagcgcagcagttgcaaaactggcacagttttatggtctgccgagctatgttgcaggtagcagcgatgcaaaagttccggatgatcaggcaggtcatgaaaaaaccatgaccaccctgctgccggcactggcaggtgcaaataccatttatggtgcaggtatgctggaactgggtatgacctttagcatggaacagctggttattgataatgatatttttagcatggttaaaaaagcaatgcagggtattccggttagcgaagaaaccctggcagttgaaagcattcagaaagttggtattggtaataattttctggcactgaaacagacccgtcagctggttgattatccgagcaatccgatgctgctggatcgtcatatgtttggtgattgggcagcagcaggtagcaaagatctggcaaccgttgcacatgaaaaagttgaagatgttctgaaaaatcatcaggttaccccgattgatgcagatatttttaaagatatgcaggcaattgttgataaagcagataaagcatttcgtggtatgtactagtagcggccgctgcagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
BBa_K727002_sequence
1
gaattcgcggccgcttctagatgatggcaaccgaatatgcactgcgtatgggtgatggtaaacgtatttatctgaccaaagaaaaaattctgagcgaaattgaagcaggtagcagcaatgcagcagatctgggtgatattccggatctgagcaccgatgaaatgaaaaaactggcagaaattctgatgatgccgggtaaagcagttagcgttgaacagggtatggaagttccggttacccatgatattggtaccattcgtctggatggtgatcagggtaatagcggtgttggtattccgagcagccgtctggttggttgtatgacccatgaacgtgcatttggtgcagataccatggaactgggtcatattgattatagctttaaaccggttaaaccggttgttagcaatgaatgtcaggcaatggaagtttgtcagcagaatatgattattccgctgttttatggtgcaatgccgaatatgggtctgtattataccccggatggtccgtttgaaaatccgggtgatctgatgaaaatgtttaaaattgataaagcacgtgaaagcatggaacatgcagcagatcatctgacccgtgataccgtttgggttatgcagaaactgtttgcaagcggtgcagatggtgttaattttgataccaccgcagcagcaggtgatgcagatatgtatggtaccctgcgtgcagttgaagttctgcgtgcacagtttccggaaatgtatattgaagttggtatggcaggtgaaatggttctgggtatgcatggtgaactggaatatgatgaagttcgtctggcaggtctgtggccgcatgaacaggcaccgctgattgcaaaagcaggtgcaaatgtttttggtccggttgttaataccaataccagcaaaaccagcgcatggaatctggcacgtgcagttacctttattaaagaagcagttaaagcaagcccgattccgtgtcatgttaatatgggtatgggtgttggtggtattccgatgctggaaaccccgccggttgatgcagttacccgtgcaagcaaagcaatggttgaagttgcaggtgttgatggtattattggtgttggtgatccgctgggtatgccgattagccatattatggcaagcggtatgaccggtattcgtgcagcaggtgatctggttgcacgtatggaatttagcaaaaatatgcgtattggtgaagcaaaagaatatgttgcaaaaaaactgaatgttgataccatggatctggcagatgaacatgttatgcgtgaactgcgtgaagaactggatattggtgttattaccagcgttccgggtgcagcaaaaggtattgcagcaaaaatgaatattgaaaaactgctgggtattaaaattaatagctgtgaaacctttcgtgcacagctggcatactagtagcggccgctgcag
BBa_C0062_sequence
1
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z