BBa_K727272
type: ComponentDefinition
persistent identity: BBa K727272
display id: BBa_K727272
version: 1
was generated by: iGEM to SBOL conversion
description: Fish Odor Generator-Complete System
created: 2012-06-15T11:00:00Z
modified: 2015-05-08T01:13:05Z
mutable provenance: Genom of
owned by: james
owned by: myers
mutable description: TMT (trimethylamine transferase) takes part in the third reaction of the production of TMA (trimethylamine) which is the major cause of unpleasant fish odor. We added Lux cassette right promoter that up-regulated by HSL and LuxR in the course of being able to regulate easily. We intended to generate a negative feedback response with the help of producing LuxR and HSL and activating the promoter with them.
discontinued: false
dominant: false
group u list: _973_
m user id: 0
owner id: 11153
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Optimized for E.coli
star: false
creator: İBRAHİM YASİR ORHAN
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_R0063
component: BBa_K727002
component: BBa_K727001
component: BBa_C0062
component: BBa_K727003
component: BBa_C0061
sequence annotation: BBa_R0063
sequence annotation: BBa_K727001
sequence annotation: BBa_K727002
sequence annotation: BBa_K727003
sequence annotation: BBa_C0062
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_K727272_sequence/1

BBa_R0063
type: Component
persistent identity: component2264442
display id: component2264442
version: 1
top level: BBa_K727272
definition: lux pL
access: public

BBa_K727002
type: Component
persistent identity: component2264450
display id: component2264450
version: 1
top level: BBa_K727272
definition: BBa_K727002
access: public

BBa_K727001
type: Component
persistent identity: component2264448
display id: component2264448
version: 1
top level: BBa_K727272
definition: BBa_K727001
access: public

BBa_C0062
type: Component
persistent identity: component2264455
display id: component2264455
version: 1
top level: BBa_K727272
definition: luxr
access: public

BBa_K727003
type: Component
persistent identity: component2264452
display id: component2264452
version: 1
top level: BBa_K727272
definition: BBa_K727003
access: public

BBa_C0061
type: Component
persistent identity: component2264460
display id: component2264460
version: 1
top level: BBa_K727272
definition: luxI
access: public

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2264442
display id: annotation2264442
version: 1
top level: BBa_K727272
location: https://synbiohub.org/public/igem/BBa_K727272/annotation2264442/range2264442/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_K727272/annotation2264442/range2264442/1
type: Range
persistent identity: range2264442
display id: range2264442
version: 1
top level: BBa_K727272
direction: direction/forward
start: 1
end: 151
orientation: inline

BBa_K727001
type: SequenceAnnotation
persistent identity: annotation2264448
display id: annotation2264448
version: 1
top level: BBa_K727272
location: https://synbiohub.org/public/igem/BBa_K727272/annotation2264448/range2264448/1
component: BBa_K727001

https://synbiohub.org/public/igem/BBa_K727272/annotation2264448/range2264448/1
type: Range
persistent identity: range2264448
display id: range2264448
version: 1
top level: BBa_K727272
direction: direction/reverse
start: 160
end: 1574
orientation: inline

BBa_K727002
type: SequenceAnnotation
persistent identity: annotation2264450
display id: annotation2264450
version: 1
top level: BBa_K727272
location: https://synbiohub.org/public/igem/BBa_K727272/annotation2264450/range2264450/1
component: BBa_K727002

https://synbiohub.org/public/igem/BBa_K727272/annotation2264450/range2264450/1
type: Range
persistent identity: range2264450
display id: range2264450
version: 1
top level: BBa_K727272
direction: direction/reverse
start: 1583
end: 3024
orientation: inline

BBa_K727003
type: SequenceAnnotation
persistent identity: annotation2264452
display id: annotation2264452
version: 1
top level: BBa_K727272
location: https://synbiohub.org/public/igem/BBa_K727272/annotation2264452/range2264452/1
component: BBa_K727003

https://synbiohub.org/public/igem/BBa_K727272/annotation2264452/range2264452/1
type: Range
persistent identity: range2264452
display id: range2264452
version: 1
top level: BBa_K727272
direction: direction/reverse
start: 3033
end: 4555
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2264455
display id: annotation2264455
version: 1
top level: BBa_K727272
location: https://synbiohub.org/public/igem/BBa_K727272/annotation2264455/range2264455/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K727272/annotation2264455/range2264455/1
type: Range
persistent identity: range2264455
display id: range2264455
version: 1
top level: BBa_K727272
direction: direction/forward
start: 4562
end: 5317
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2264460
display id: annotation2264460
version: 1
top level: BBa_K727272
location: https://synbiohub.org/public/igem/BBa_K727272/annotation2264460/range2264460/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K727272/annotation2264460/range2264460/1
type: Range
persistent identity: range2264460
display id: range2264460
version: 1
top level: BBa_K727272
direction: direction/forward
start: 5349
end: 5966
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

lux pL
type: ComponentDefinition
persistent identity: BBa R0063
display id: BBa_R0063
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pL)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"> Unspecified.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR/HSL
sequence annotation: BBa_R0063
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence annotation: Putative LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0063_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2051
display id: annotation2051
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
type: Range
persistent identity: range2051
display id: range2051
version: 1
top level: lux pL
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation7071
display id: annotation7071
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
type: Range
persistent identity: range7071
display id: range7071
version: 1
top level: lux pL
direction: direction/forward
start: 1
end: 151
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2053
display id: annotation2053
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
type: Range
persistent identity: range2053
display id: range2053
version: 1
top level: lux pL
direction: direction/forward
start: 89
end: 94
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2052
display id: annotation2052
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
type: Range
persistent identity: range2052
display id: range2052
version: 1
top level: lux pL
direction: direction/forward
start: 115
end: 122
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2054
display id: annotation2054
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
type: Range
persistent identity: range2054
display id: range2054
version: 1
top level: lux pL
direction: direction/forward
start: 128
end: 128
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2055
display id: annotation2055
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
type: Range
persistent identity: range2055
display id: range2055
version: 1
top level: lux pL
direction: direction/forward
start: 130
end: 149
orientation: inline

BBa_K727001
type: ComponentDefinition
persistent identity: BBa K727001
display id: BBa_K727001
version: 1
was generated by: iGEM to SBOL conversion
description: Monomethylamine Methyltransferase (MMT)
created: 2012-06-15T11:00:00Z
modified: 2015-05-08T01:13:05Z
mutable provenance: Genome of
owned by: james
owned by: myers
mutable description: MMT (monomethylamine transferase) catalyses the first reaction of TMA (trimethylamine) synthesis. TMA is the major cause of unpleasant fish odor. For additional information: http://2012hs.igem.org/Team:AUC_Turkey/Project/TMA
discontinued: false
dominant: false
experience: experience/None
group u list: _973_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Optimized for E.coli
star: false
creator: Mustafa Elitok
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_K727001
sequence annotation: MMT
sequence: https://synbiohub.org/public/igem/BBa_K727001_sequence/1

BBa_K727001
type: SequenceAnnotation
persistent identity: annotation2176578
display id: annotation2176578
version: 1
top level: BBa_K727001
location: https://synbiohub.org/public/igem/BBa_K727001/annotation2176578/range2176578/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K727001/annotation2176578/range2176578/1
type: Range
persistent identity: range2176578
display id: range2176578
version: 1
top level: BBa_K727001
direction: direction/reverse
start: 1
end: 1415
orientation: inline

MMT
type: SequenceAnnotation
persistent identity: annotation2176577
display id: annotation2176577
version: 1
top level: BBa_K727001
location: https://synbiohub.org/public/igem/BBa_K727001/annotation2176577/range2176577/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K727001/annotation2176577/range2176577/1
type: Range
persistent identity: range2176577
display id: range2176577
version: 1
top level: BBa_K727001
direction: direction/forward
start: 1
end: 1415
orientation: inline

BBa_K727002
type: ComponentDefinition
persistent identity: BBa K727002
display id: BBa_K727002
version: 1
was generated by: iGEM to SBOL conversion
description: Dimethylamine Methyltransferase (DMT)
created: 2012-06-15T11:00:00Z
modified: 2015-05-08T01:13:05Z
mutable provenance: Genome of
owned by: james
owned by: myers
mutable description: DMT (monomethylamine transferase) catalyses the second reaction of TMA (trimethylamine) synthesis. TMA is the major cause of unpleasant fish odor.
discontinued: false
dominant: false
experience: experience/None
group u list: _973_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Optimized for E.coli
star: false
creator: Mustafa Elitok
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: DMT
sequence: https://synbiohub.org/public/igem/BBa_K727002_sequence/1

DMT
type: SequenceAnnotation
persistent identity: annotation2176576
display id: annotation2176576
version: 1
top level: BBa_K727002
location: https://synbiohub.org/public/igem/BBa_K727002/annotation2176576/range2176576/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K727002/annotation2176576/range2176576/1
type: Range
persistent identity: range2176576
display id: range2176576
version: 1
top level: BBa_K727002
direction: direction/forward
start: 1
end: 1442
orientation: inline

BBa_K727003
type: ComponentDefinition
persistent identity: BBa K727003
display id: BBa_K727003
version: 1
was generated by: iGEM to SBOL conversion
description: Trimethylamine Methyltransferase (TMT)
created: 2012-06-15T11:00:00Z
modified: 2015-05-08T01:13:05Z
mutable provenance: Genome of
owned by: james
owned by: myers
mutable description: TMT (trimethylamine transferase) takes part in the third reaction of the production of TMA (trimethylamine) which is the major cause of unpleasant fish odor.
discontinued: false
dominant: false
experience: experience/None
group u list: _973_
m user id: 0
owner id: 11153
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Optimized for E.coli
star: false
creator: İBRAHİM YASİR ORHAN
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: TMT
sequence: https://synbiohub.org/public/igem/BBa_K727003_sequence/1

TMT
type: SequenceAnnotation
persistent identity: annotation2176257
display id: annotation2176257
version: 1
top level: BBa_K727003
location: https://synbiohub.org/public/igem/BBa_K727003/annotation2176257/range2176257/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K727003/annotation2176257/range2176257/1
type: Range
persistent identity: range2176257
display id: range2176257
version: 1
top level: BBa_K727003
direction: direction/forward
start: 1
end: 1523
orientation: inline

https://synbiohub.org/public/igem/BBa_K727272_sequence/1
type: Sequence
persistent identity: BBa K727272 sequence
display id: BBa_K727272_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagaggaattcgcggccgcttctagatgatgacctttcgtaaatattttgattgttatgatttttatgatcgtgcaaaagttggtgaaaaatgtaccctggatgattgggatctgatgaaaattccgatgaaagcaatggaactgaaacagaaatatggtctggattttaaaggtgaatttattccgaccgataaagatatgatggaaaaactgtttaaagcaggttttgaaatgctgctggaatgtggtatttattgtaccgatacccatcgtattgttaaatataccgaagatgaaatttgggatgcaattaataatgttcagaaagaatttgttctgggtaccggtcgtgatgcagttaatgttcgtaaacgtagcgttggtgataaagcaaaaccgattgttcagggtggtccgaccggtagcccgattagcgaagatgtttttatgccggttcatatgagctatgcactggaaaaagaagttgataccattgttaatggtgttatgaccaccgttcgtggtaaagcaccgattccgaaaagcccgtatgaagttctggcagcaaaaaccgaaacccgtctgattaaaaatgcatgtgcaatggcaggtcgtccgggtatgggtgttggtccggaaaccagcctgagcgcacagggtaatattagcgcagattgtgcaggtggtatgacctgtaccgatagccatgaagttagccagctgtgtgaactgaaaattgatctggatgcaattagcgttattgcacattataccgcaaatagcgatattattatggatgaacagatgccgatttttggtggttatgcaggtggtattgaagaaaccaccattgttgatgttgcaacccatattaatgcagttctgatgagcagcgcaagctggcatctggatggtccggttcatattcgttggggtagcaccaatacccgtgaaaccctgatgattgcaggttgggcatgtgcaaccattagcgaatttaccgatattctgagcggtaatcagtattatccgtgtgcaggtccgtgtaccgaaatgtgtctgctggaagcaagcgcacagagcattaccgataccgcaagcggtcgtgaaattctgagcggtgttgcaagcgcaaaaggtgttgttaccgataaaaccaccggtatggaagcacgtatgatgggtgaagttgcacgtgcaaccgcaggtgttgaaattagcgaagttaatgttattctggataaactggttgcactgtatgaaaaaaattatgcaagcgcaccggcaggtaaaacctttcaggaatgttatgatgttaaaaccgttaccccgaccgatgaatatatgcaggtttatgatggtgcacgtaaaaaactggaagatctgggtctggttttttactagtagcggccgctgcagtactagaggaattcgcggccgcttctagatgatggcaaccgaatatgcactgcgtatgggtgatggtaaacgtatttatctgaccaaagaaaaaattctgagcgaaattgaagcaggtagcagcaatgcagcagatctgggtgatattccggatctgagcaccgatgaaatgaaaaaactggcagaaattctgatgatgccgggtaaagcagttagcgttgaacagggtatggaagttccggttacccatgatattggtaccattcgtctggatggtgatcagggtaatagcggtgttggtattccgagcagccgtctggttggttgtatgacccatgaacgtgcatttggtgcagataccatggaactgggtcatattgattatagctttaaaccggttaaaccggttgttagcaatgaatgtca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0063_sequence/1
type: Sequence
persistent identity: BBa R0063 sequence
display id: BBa_R0063_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K727001_sequence/1
type: Sequence
persistent identity: BBa K727001 sequence
display id: BBa_K727001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagatgatgacctttcgtaaatattttgattgttatgatttttatgatcgtgcaaaagttggtgaaaaatgtaccctggatgattgggatctgatgaaaattccgatgaaagcaatggaactgaaacagaaatatggtctggattttaaaggtgaatttattccgaccgataaagatatgatggaaaaactgtttaaagcaggttttgaaatgctgctggaatgtggtatttattgtaccgatacccatcgtattgttaaatataccgaagatgaaatttgggatgcaattaataatgttcagaaagaatttgttctgggtaccggtcgtgatgcagttaatgttcgtaaacgtagcgttggtgataaagcaaaaccgattgttcagggtggtccgaccggtagcccgattagcgaagatgtttttatgccggttcatatgagctatgcactggaaaaagaagttgataccattgttaatggtgttatgaccaccgttcgtggtaaagcaccgattccgaaaagcccgtatgaagttctggcagcaaaaaccgaaacccgtctgattaaaaatgcatgtgcaatggcaggtcgtccgggtatgggtgttggtccggaaaccagcctgagcgcacagggtaatattagcgcagattgtgcaggtggtatgacctgtaccgatagccatgaagttagccagctgtgtgaactgaaaattgatctggatgcaattagcgttattgcacattataccgcaaatagcgatattattatggatgaacagatgccgatttttggtggttatgcaggtggtattgaagaaaccaccattgttgatgttgcaacccatattaatgcagttctgatgagcagcgcaagctggcatctggatggtccggttcatattcgttggggtagcaccaatacccgtgaaaccctgatgattgcaggttgggcatgtgcaaccattagcgaatttaccgatattctgagcggtaatcagtattatccgtgtgcaggtccgtgtaccgaaatgtgtctgctggaagcaagcgcacagagcattaccgataccgcaagcggtcgtgaaattctgagcggtgttgcaagcgcaaaaggtgttgttaccgataaaaccaccggtatggaagcacgtatgatgggtgaagttgcacgtgcaaccgcaggtgttgaaattagcgaagttaatgttattctggataaactggttgcactgtatgaaaaaaattatgcaagcgcaccggcaggtaaaacctttcaggaatgttatgatgttaaaaccgttaccccgaccgatgaatatatgcaggtttatgatggtgcacgtaaaaaactggaagatctgggtctggttttttactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K727002_sequence/1
type: Sequence
persistent identity: BBa K727002 sequence
display id: BBa_K727002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagatgatggcaaccgaatatgcactgcgtatgggtgatggtaaacgtatttatctgaccaaagaaaaaattctgagcgaaattgaagcaggtagcagcaatgcagcagatctgggtgatattccggatctgagcaccgatgaaatgaaaaaactggcagaaattctgatgatgccgggtaaagcagttagcgttgaacagggtatggaagttccggttacccatgatattggtaccattcgtctggatggtgatcagggtaatagcggtgttggtattccgagcagccgtctggttggttgtatgacccatgaacgtgcatttggtgcagataccatggaactgggtcatattgattatagctttaaaccggttaaaccggttgttagcaatgaatgtcaggcaatggaagtttgtcagcagaatatgattattccgctgttttatggtgcaatgccgaatatgggtctgtattataccccggatggtccgtttgaaaatccgggtgatctgatgaaaatgtttaaaattgataaagcacgtgaaagcatggaacatgcagcagatcatctgacccgtgataccgtttgggttatgcagaaactgtttgcaagcggtgcagatggtgttaattttgataccaccgcagcagcaggtgatgcagatatgtatggtaccctgcgtgcagttgaagttctgcgtgcacagtttccggaaatgtatattgaagttggtatggcaggtgaaatggttctgggtatgcatggtgaactggaatatgatgaagttcgtctggcaggtctgtggccgcatgaacaggcaccgctgattgcaaaagcaggtgcaaatgtttttggtccggttgttaataccaataccagcaaaaccagcgcatggaatctggcacgtgcagttacctttattaaagaagcagttaaagcaagcccgattccgtgtcatgttaatatgggtatgggtgttggtggtattccgatgctggaaaccccgccggttgatgcagttacccgtgcaagcaaagcaatggttgaagttgcaggtgttgatggtattattggtgttggtgatccgctgggtatgccgattagccatattatggcaagcggtatgaccggtattcgtgcagcaggtgatctggttgcacgtatggaatttagcaaaaatatgcgtattggtgaagcaaaagaatatgttgcaaaaaaactgaatgttgataccatggatctggcagatgaacatgttatgcgtgaactgcgtgaagaactggatattggtgttattaccagcgttccgggtgcagcaaaaggtattgcagcaaaaatgaatattgaaaaactgctgggtattaaaattaatagctgtgaaacctttcgtgcacagctggcatactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K727003_sequence/1
type: Sequence
persistent identity: BBa K727003 sequence
display id: BBa_K727003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagatggcaaaaaataatgcagttgcaggttttaatgcactgaatggtgttgaactgaatctgtttaccaccgatgaactgaaagcaattcattatgcaaccatggaagttctgatggatccgggtattcaggttagcgatccggaagcacgtcagatttttaaagaaaatggttgtgaagttaatgaaaaaaccaatgttgttaaaattccggaatatctggttcgtaaagcattacagctggcaccgagccgttttgttctgtggggtcgtgataaaaaatttaataccgttcaggaatgtggtggtaaagttcattggacctgttttggtaccggtgttaaagtttgtaaatatcaggatggtaaatatgttaccgttgatagcgttgaaaaagatattgcagatattgcaaaactgtgtgattgggcagaaaatattgattattttagcctgccggttagcgcacgtgatattgcaggtcagggtgcacaggatgttcatgaaaccctgaccccgctggcaaataccgcaaaacattttcatcatattgatccggttggtgaaaatgttgaatattatcgtgatattgttaaagcatattatggtggtgatgaagaagaagcacgtaaaaaaccgatttttagcatgctgctgtgtccgaccagcccgctggaactgagcgttaatgcatgtcaggttattattaaaggtgcacgttttggtattccggttaatgttctgagcatggcaatgagcggtggtagcagcccggtttatctggcaggtaccctggttacccataatgcagaagttctgagcggtattgttctggcacagctgaccgttccgggtgcaaaagtttggtatggtagcagcaccaccacctttgatctgaaaaaaggtaccgcaccggttggtagcccggaactgggtctgattagcgcagcagttgcaaaactggcacagttttatggtctgccgagctatgttgcaggtagcagcgatgcaaaagttccggatgatcaggcaggtcatgaaaaaaccatgaccaccctgctgccggcactggcaggtgcaaataccatttatggtgcaggtatgctggaactgggtatgacctttagcatggaacagctggttattgataatgatatttttagcatggttaaaaaagcaatgcagggtattccggttagcgaagaaaccctggcagttgaaagcattcagaaagttggtattggtaataattttctggcactgaaacagacccgtcagctggttgattatccgagcaatccgatgctgctggatcgtcatatgtttggtgattgggcagcagcaggtagcaaagatctggcaaccgttgcacatgaaaaagttgaagatgttctgaaaaatcatcaggttaccccgattgatgcagatatttttaaagatatgcaggcaattgttgataaagcagataaagcatttcgtggtatgtactagtagcggccgctgcag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z