BBa_R0062 1 lux pR Promoter (luxR & HSL regulated -- lux pR) 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z <em>V. fischeri</em> Released HQ 2013 Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription. false true _1_ 0 24 7 In stock false <P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation7070 1 BBa_R0062 range7070 1 1 55 annotation2046 1 -35 range2046 1 20 25 annotation2047 1 -10 range2047 1 42 47 annotation2048 1 start range2048 1 53 53 annotation2045 1 LuxR/HSL range2045 1 1 20 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K727503 1 BBa_K727503 Geraniol Synthase Coding Device 2012-06-15T11:00:00Z 2015-05-08T01:13:05Z Genome of GS (Geraniol syntase) synthesizes Geraniol which is the second reaction of geraniol production. false false _973_ 0 11153 9 Not in stock false Optimized for E.coli false &#304;BRAH&#304;M YAS&#304;R ORHAN component2269162 1 BBa_K727006 component2269160 1 BBa_J09855 annotation2269162 1 BBa_K727006 range2269162 1 1053 2902 annotation2269160 1 BBa_J09855 range2269160 1 1 1044 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J09855 1 BBa_J09855 Constitutive luxR device with pLuxR 2006-10-25T11:00:00Z 2015-08-31T04:08:21Z This part was constructed from registry biobricks. Released HQ 2013 I14032.I0462.R0062 false true _72_40_ 0 475 72 In stock true This is a universal part designed to allow induction by HSL of whatever is assembled downstream of the part. true Jon Badalamenti component2260430 1 BBa_I14032 component2260445 1 BBa_I0462 component2260447 1 BBa_R0062 annotation2260430 1 BBa_I14032 range2260430 1 1 37 annotation2260447 1 BBa_R0062 range2260447 1 990 1044 annotation2260445 1 BBa_I0462 range2260445 1 46 981 BBa_I14032 1 P(Lac) IQ promoter P(Lac) IQ 2004-08-03T11:00:00Z 2015-08-31T04:07:37Z Plasmid pMAL-p2X Released HQ 2013 Constitutive Promoter, High Transcription false true _4_ 0 171 7 In stock false true Vikram Vijayan, Allen Hsu, Lawrence Fomundam annotation1028342 1 P(Lac) IQ range1028342 1 1 37 annotation1028343 1 -35 range1028343 1 3 8 annotation1028344 1 -10 range1028344 1 26 31 BBa_I0462 1 LuxR luxR Protein Generator 2003-12-04T12:00:00Z 2015-08-31T04:07:29Z Released HQ 2013 Produces LuxR protein which can sense 3OC₆HSL in the media and activate transcription from R0062, the right hand Lux promoter false false _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff component943166 1 BBa_B0034 component943181 1 BBa_C0062 component943189 1 BBa_B0010 component943199 1 BBa_B0012 annotation943189 1 BBa_B0010 range943189 1 808 887 annotation943199 1 BBa_B0012 range943199 1 896 936 annotation943166 1 BBa_B0034 range943166 1 1 12 annotation943181 1 BBa_C0062 range943181 1 19 774 BBa_K727006 1 BBa_K727006 Geraniol Synthase 2012-06-15T11:00:00Z 2015-05-08T01:13:05Z Genom on GS (Geraniol syntase) synthesizes Geraniol which is the second reaction of geraniol production. false false _973_ 0 11153 9 Not in stock true Optimized for E.coli false &#304;BRAH&#304;M YAS&#304;R ORHAN annotation2176260 1 GS range2176260 1 1 1850 BBa_C0062 1 luxr luxR repressor/activator, (no LVA?) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z <em>V. fischeri</em> <genbank>AF170104</genbank> Released HQ 2013 In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p> false true _1_ 0 24 7 In stock false <P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation2213986 1 Help:Barcodes range2213986 1 757 781 annotation7039 1 BBa_C0062 range7039 1 1 756 annotation1766 1 luxR range1766 1 1 750 annotation1764 1 T range1764 1 174 174 annotation1765 1 A range1765 1 492 492 annotation1762 1 prefix range1762 1 1 2 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_R0062_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa BBa_B0034_sequence 1 aaagaggagaaa BBa_J09855_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa BBa_I14032_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcc BBa_I0462_sequence 1 aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K727006_sequence 1 gaattcgcggccgcttctagatgagcagcattagccagaaagttgttattggtctgaataaagcagcagcaaataataatttacagaatctggatcgtcgtggttttaaaacccgttgtgttagcagcagcaaagcagcaagctgtctgcgtgcaagctgtagcttacagctggatgttaaaccggttcaggaaggtcgtcgtagcggtaattatcagccgagcatttgggattttaattatgttcagagcctgaataccccgtataaagaagaacgttatctgacccgtcatgcagaactgattgttcaggttaaaccgctgctggaaaaaaaaatggaaccggcacagcagctggaactgattgatgatctgaataatctgggtctgagctatttttttcaggatcgtattaaacagattctgagctttatttatgatgaaaatcagtgttttcatagcaatattaatgatcaggcagaaaaacgtgatctgtattttaccgcactgggttttcgtctgctgcgtcagcatggttttgatgttagccaggaagtttttgattgttttaaaaatgataatggtagcgattttaaagcaagcctgagcgataataccaaaggtctgttacagctgtatgaagcaagctttctggttcgtgaaggtgaagataccctggaacaggcacgtcagtttgcaaccaagtttctgcgtcgtaaactggatgaaattgatgataatcatctgctgagctgtattcatcatagcctggagattccgctgcattggcgtattcagcgtctggaagcacgttggtttctggatgcatatgcaacccgtcatgatatgaatccggttattctggaactggcaaaactggattttaatattattcaggcaacccatcaggaagaactgaaagatgttagccgttggtggcagaatacccgtctggcagaaaaactgccgtttgttcgtgatcgtctggttgaaagctatttttgggcaattgcactgtttgaaccgcatcagtatggttatcagcgtcgtgttgcagcaaaaattattaccctggcaaccagcattgatgatgtttatgatatttatggtaccctggatgaattacagctgtttaccgataattttcgtcgttgggataccgaaagcctgggtcgtctgccgtatagcatgcagctgttttatatggttattcataattttgttagcgaactggcatatgagattctgaaagaaaaaggttttattgttattccgtatttacagcgtagctgggttgatctggcagaaagctttctgaaagaagcaaattggtattatagcggttataccccgagcctggaagaatatattgataatggtagcattagcattggtgcagttgcagttctgagccaggtttattttaccctggcaaatagcattgaaaaaccgaaaattgaaagcatgtataaatatcatcatattctgcgtctgagcggtctgctggttcgtctgcatgatgatctgggtaccagcctgtttgaaaaaaaacgtggtgatgttccgaaagcagttgagatttgtatgaaagaacgtaatgttaccgaagaagaagcagaagaacatgttaaatatctgattcgtgaagcatggaaagaaatgaataccgcaaccaccgcagcaggttgtccgtttatggatgaactgaatgttgcagcagcaaatctgggtcgtgcagcacagtttgtttatctggatggtgatggtcatggtgttcagcatagcaagattcatcagcagatgggtggtctgatgtttgaaccgtatgtttactagtagcggccgctgcag BBa_K727503_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagaggaattcgcggccgcttctagatgagcagcattagccagaaagttgttattggtctgaataaagcagcagcaaataataatttacagaatctggatcgtcgtggttttaaaacccgttgtgttagcagcagcaaagcagcaagctgtctgcgtgcaagctgtagcttacagctggatgttaaaccggttcaggaaggtcgtcgtagcggtaattatcagccgagcatttgggattttaattatgttcagagcctgaataccccgtataaagaagaacgttatctgacccgtcatgcagaactgattgttcaggttaaaccgctgctggaaaaaaaaatggaaccggcacagcagctggaactgattgatgatctgaataatctgggtctgagctatttttttcaggatcgtattaaacagattctgagctttatttatgatgaaaatcagtgttttcatagcaatattaatgatcaggcagaaaaacgtgatctgtattttaccgcactgggttttcgtctgctgcgtcagcatggttttgatgttagccaggaagtttttgattgttttaaaaatgataatggtagcgattttaaagcaagcctgagcgataataccaaaggtctgttacagctgtatgaagcaagctttctggttcgtgaaggtgaagataccctggaacaggcacgtcagtttgcaaccaagtttctgcgtcgtaaactggatgaaattgatgataatcatctgctgagctgtattcatcatagcctggagattccgctgcattggcgtattcagcgtctggaagcacgttggtttctggatgcatatgcaacccgtcatgatatgaatccggttattctggaactggcaaaactggattttaatattattcaggcaacccatcaggaagaactgaaagatgttagccgttggtggcagaatacccgtctggcagaaaaactgccgtttgttcgtgatcgtctggttgaaagctatttttgggcaattgcactgtttgaaccgcatcagtatggttatcagcgtcgtgttgcagcaaaaattattaccctggcaaccagcattgatgatgtttatgatatttatggtaccctggatgaattacagctgtttaccgataattttcgtcgttgggataccgaaagcctgggtcgtctgccgtatagcatgcagctgttttatatggttattcataattttgttagcgaactggcatatgagattctgaaagaaaaaggttttattgttattccgtatttacagcgtagctgggttgatctggcagaaagctttctgaaagaagcaaattggtattatagcggttataccccgagcctggaagaatatattgataatggtagcattagcattggtgcagttgcagttctgagccaggtttattttaccctggcaaatagcattgaaaaaccgaaaattgaaagcatgtataaatatcatcatattctgcgtctgagcggtctgctggttcgtctgcatgatgatctgggtaccagcctgtttgaaaaaaaacgtggtgatgttccgaaagcagttgagatttgtatgaaagaacgtaatgttaccgaagaagaagcagaagaacatgttaaatatctgattcgtgaagcatggaaagaaatgaataccgcaaccaccgcagcaggttgtccgtttatggatgaactgaatgttgcagcagcaaatctgggtcgtgcagcacagtttgtttatctggatggtgatggtcatggtgttcagcatagcaagattcatcagcagatgggtggtctgatgtttgaaccgtatgtttactagtagcggccgctgcag BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_C0062_sequence 1 atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z